HSD17B6 (NM_003725) Human Untagged Clone

CAT#: SC324525

HSD17B6 (untagged)-Human hydroxysteroid (17-beta) dehydrogenase 6 homolog (mouse) (HSD17B6)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HSD17B6
• Synonyms: HSE; RODH; SDR9C6
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_003725.2 AGCAGTTGGGGCAGGAGGAAGCCGACTGCTGCCTGGTCTGCAAAGAAGTCCTTTCAAGTC
  TCTAGGACTGGACTCTTCCTAAGCAAGTCCGAGAAGGAAGCACCCTCACTATGTGGCTCT
  ACCTGGCGGCCTTCGTGGGCCTGTACTACCTTCTGCACTGGTACCGGGAGAGGCAGGTGG
  TGAGCCACCTCCAAGACAAGTATGTCTTTATCACGGGCTGTGACTCGGGCTTTGGGAACC
  TGCTGGCCAGACAGCTGGATGCACGAGGCTTGAGAGTGCTGGCTGCGTGTCTGACGGAGA
  AGGGGGCCGAGCAGCTGAGGGGCCAGACGTCTGACAGGCTGGAGACGGTGACCCTGGATG
  TTACCAAGATGGAGAGCATCGCTGCAGCTACTCAGTGGGTGAAGGAGCATGTGGGGGACA
  GAGGACTCTGGGGACTGGTGAACAATGCAGGCATTCTTACACCAATTACCTTATGTGAGT
  GGCTGAACACTGAGGACTCTATGAATATGCTCAAAGTGAACCTCATTGGTGTGATCCAGG
  TGACCTTGAGCATGCTTCCTTTGGTGAGGAGAGCACGGGGAAGAATTGTCAATGTCTCCA
  GCATTCTGGGAAGAGTTGCTTTCTTTGTAGGAGGCTACTGTGTCTCCAAGTATGGAGTGG
  AAGCCTTTTCAGATATTCTGAGGCGTGAGATTCAACATTTTGGGGTGAAAATCAGCATAG
  TTGAACCTGGCTACTTCAGAACGGGAATGACAAACATGACACAGTCCTTAGAGCGAATGA
  AGCAAAGTTGGAAAGAAGCCCCCAAGCATATTAAGGAGACCTATGGACAGCAGTATTTTG
  ATGCCCTTTACAATATCATGAAGGAAGGGCTGTTGAATTGTAGCACAAACCTGAACCTGG
  TCACTGACTGCATGGAACATGCTCTGACATCGGTGCATCCGCGAACTCGATATTCAGCTG
  GCTGGGATGCTAAATTTTTCTTCATCCCTCTATCTTATTTACCTACATCACTGGCAGACT
  ACATTTTGACTAGATCTTGGCCCAAACCAGCCCAGGCAGTCTAAAGAAAACTGGGTTGGT
  GCTTCTTGGAATGAAGGCAAAAATCTGAAATTGTTAGTGTCTCAGTAATCCTGATTTAGA
  ACCCAGGCTTTTTGTAACAATGTGTTTTCTTGCCTAAATTCATTTATCTGGCATCATCAG
  AGTACTAACATGTTTATATTTCAGATATCCAAAGCTTACCACTTTAGGTGATGAATCTTT
  ACTATTTTAGCCCTTTTTTGATGAGACTATTTGTCTAAAGTGAATCATTTGTTCTTGCCT
  TATTAAACAGAGTAGATGGAAAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_003725
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003725.2, NP_003716.2
• RefSeq Size: 1512 bp
• RefSeq ORF: 954 bp
• Locus ID: 8630
• UniProt ID: O14756
• Cytogenetics: 12q13.3
• Domains: adh_short
• Protein Families: Druggable Genome
• Gene Summary: The protein encoded by this gene has both oxidoreductase and epimerase activities and is involved in androgen catabolism. The oxidoreductase activity can convert 3 alpha-adiol to dihydrotestosterone, while the epimerase activity can convert androsterone to epi-androsterone. Both reactions use NAD+ as the preferred cofactor. This gene is a member of the retinol dehydrogenase family. [provided by RefSeq, Aug 2013]