IMP3 (IGF2BP3) (NM_006547) Human Untagged Clone

CAT#: SC324059

IGF2BP3 (untagged)-Human insulin-like growth factor 2 mRNA binding protein 3 (IGF2BP3)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: IMP3
• Synonyms: CT98; IMP-3; IMP3; KOC; KOC1; VICKZ3
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_006547.2 GTGCGTTTGGGTTGTAGCTAGGCTTTTTCTTTTCTTTCTCTTTTAAAACACATCTAGACA
  AGGAAAAAACAAGCCTCGGATCTGATTTTTCACTCCTCGTTCTTGTGCTTGGTTCTTACT
  GTGTTTGTGTATTTTAAAGGCGAGAAGACGAGGGGAACAAAACCAGCTGGATCCATCCAT
  CACCGTGGGTGGTTTTAATTTTTCGTTTTTTCTCGTTATTTTTTTTTAAACAACCACTCT
  TCACAATGAACAAACTGTATATCGGAAACCTCAGCGAGAACGCCGCCCCCTCGGACCTAG
  AAAGTATCTTCAAGGACGCCAAGATCCCGGTGTCGGGACCCTTCCTGGTGAAGACTGGCT
  ACGCGTTCGTGGACTGCCCGGACGAGAGCTGGGCCCTCAAGGCCATCGAGGCGCTTTCAG
  GTAAAATAGAACTGCACGGGAAACCCATAGAAGTTGAGCACTCGGTCCCAAAAAGGCAAA
  GGATTCGGAAACTTCAGATACGAAATATCCCGCCTCATTTACAGTGGGAGGTGCTGGATA
  GTTTACTAGTCCAGTATGGAGTGGTGGAGAGCTGTGAGCAAGTGAACACTGACTCGGAAA
  CTGCAGTTGTAAATGTAACCTATTCCAGTAAGGACCAAGCTAGACAAGCACTAGACAAAC
  TGAATGGATTTCAGTTAGAGAATTTCACCTTGAAAGTAGCCTATATCCCTGATGAAATGG
  CCGCCCAGCAAAACCCCTTGCAGCAGCCCCGAGGTCGCCGGGGGCTTGGGCAGAGGGGCT
  CCTCAAGGCAGGGGTCTCCAGGATCCGTATCCAAGCAGAAACCATGTGATTTGCCTCTGC
  GCCTGCTGGTTCCCACCCAATTTGTTGGAGCCATCATAGGAAAAGAAGGTGCCACCATTC
  GGAACATCACCAAACAGACCCAGTCTAAAATCGATGTCCACCGTAAAGAAAATGCGGGGG
  CTGCTGAGAAGTCGATTACTATCCTCTCTACTCCTGAAGGCACCTCTGCGGCTTGTAAGT
  CTATTCTGGAGATTATGCATAAGGAAGCTCAAGATATAAAATTCACAGAAGAGATCCCCT
  TGAAGATTTTAGCTCATAATAACTTTGTTGGACGTCTTATTGGTAAAGAAGGAAGAAATC
  TTAAAAAAATTGAGCAAGACACAGACACTAAAATCACGATATCTCCATTGCAGGAATTGA
  CGCTGTATAATCCAGAACGCACTATTACAGTTAAAGGCAATGTTGAGACATGTGCCAAAG
  CTGAGGAGGAGATCATGAAGAAAATCAGGGAGTCTTATGAAAATGATATTGCTTCTATGA
  ATCTTCAAGCACATTTAATTCCTGGATTAAATCTGAACGCCTTGGGTCTGTTCCCACCCA
  CTTCAGGGATGCCACCTCCCACCTCAGGGCCCCCTTCAGCCATGACTCCTCCCTACCCGC
  AGTTTGAGCAATCAGAAACGGAGACTGTTCATCTGTTTATCCCAGCTCTATCAGTCGGTG
  CCATCATCGGCAAGCAGGGCCAGCACATCAAGCAGCTTTCTCGCTTTGCTGGAGCTTCAA
  TTAAGATTGCTCCAGCAGAAGCACCAGATGCTAAAGTGAGGATGGTGATTATCACTGGAC
  CACCAGAGGCTCAGTTCAAGGCTCAGGGAAGAATTTATGGAAAAATTAAAGAAGAAAACT
  TTGTTAGTCCTAAAGAAGAGGTGAAACTTGAAGCTCATATCAGAGTGCCATCCTTTGCTG
  CTGGCAGAGTTATTGGAAAAGGAGGCAAAACGGTGAATGAACTTCAGAATTTGTCAAGTG
  CAGAAGTTGTTGTCCCTCGTGACCAGACACCTGATGAGAATGACCAAGTGGTTGTCAAAA
  TAACTGGTCACTTCTATGCTTGCCAGGTTGCCCAGAGAAAAATTCAGGAAATTCTGACTC
  AGGTAAAGCAGCACCAACAACAGAAGGCTCTGCAAAGTGGACCACCTCAGTCAAGACGGA
  AGTAAAGGCTCAGGAAACAGCCCACCACAGAGGCAGATGCCAAACCAAAGACAGATTGCT
  TAACCAACAGATGGGCGCTGACCCCCTATCCAGAATCACATGCACAAGTTTTTACCTAGC
  CAGTTGTTTCTGAGGACCAGGCAACTTTTGAACTCCTGTCTCTGTGAGAATGTATACTTT
  ATGCTCTCTGAAATGTATGACACCCAGCTTTAAAACAAACAAACAAAAAAAAAAAAAAA
• Restriction Sites: ECoRI-NOT
• ACCN: NM_006547
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006547.2, NP_006538.2
• RefSeq Size: 4168 bp
• RefSeq ORF: 1740 bp
• Locus ID: 10643
• UniProt ID: O00425
• Cytogenetics: 7p15.3
• Domains: RRM, KH
• Gene Summary: The protein encoded by this gene is primarily found in the nucleolus, where it can bind to the 5' UTR of the insulin-like growth factor II leader 3 mRNA and may repress translation of insulin-like growth factor II during late development. The encoded protein contains several KH domains, which are important in RNA binding and are known to be involved in RNA synthesis and metabolism. A pseudogene exists on chromosome 7, and there are putative pseudogenes on other chromosomes. [provided by RefSeq, Jul 2008]