KIAA0859 (METTL13) (NM_014955) Human Untagged Clone

CAT#: SC323939

METTL13 (untagged)-Human methyltransferase like 13 (METTL13), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: KIAA0859
• Synonyms: 5630401D24Rik; CGI-01; DFNB26; DFNB26M; DFNM1; feat; KIAA0859; METTL13
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_014955.2 CACTGCAGTGTCCCGGAGCCTGCGTGTGGTGGGCAAGCTCCTCAAATGGTATCTCACAGG
  GAATAGGGGAGTCTTGAAAACGCAGCTTCGGCAGTAGGAACATGAACCTCTTACCTAAAA
  GTTCCAGGGAGTTTGGCTCCGTTGACTATTGGGAGAAGTTCTTCCAGCAGCGAGGAAAGA
  AAGCTTTCGAGTGGTATGGAACCTACCTGGAACTGTGCGGGGTGCTACATAAATATATCA
  AGCCCAGGGAAAAGGTGCTGGTGATTGGGTGTGGCAACTCAGAACTGAGTGAGCAACTGT
  ATGATGTGGGCTATCGGGATATAGTGAACATCGACATCAGTGAGGTTGTCATCAAGCAAA
  TGAAGGAATGTAATGCCACCCGACGGCCCCAGATGAGCTTCTTGAAGATGGACATGACGC
  AGATGGAGTTTCCTGATGCCTCGTTCCAGGTGGTGTTGGACAAGGGCACCCTGGATGCTG
  TCCTGACAGATGAGGAAGAGAAGACCTTACAACAGGTGGACAGGATGCTGGCTGAGGTTG
  GCCGTGTCCTGCAGGTGGGCGGTCGCTATCTCTGCATCTCCCTGGCTCAGGCTCACATCC
  TGAAGAAAGCAGTGGGCCACTTCTCCCGGGAGGGGTGGATGGTGAGGGTGCACCAAGTGG
  CCAACAGCCAGGACCAGGTGTTGGAAGCAGAGCCTCAGTTCTCCTTGCCTGTCTTTGCCT
  TCATCATGACCAAGTTCAGGCCAGTCCCTGGCTCTGCCCTTCAGATCTTTGAGCTGTGTG
  CTCAGGAGCAGCGCAAGCCTGTGCGGCTGGAGAGTGCCGAGCGGCTGGCCGAGGCGGTGC
  AGGAGCGACAGCAGTATGCCTGGCTGTGCAGCCAGCTGCGCCGCAAGGCCAGGCTGGGGA
  GTGTGTCTCTGGACTTGTGCGATGGGGACACGGGGGAGCCACGCTACACCCTCCACGTGG
  TGGACAGCCCCACTGTGAAACCATCGCGGGACAATCATTTTGCGATTTTCATCATCCCTC
  AGGGCCGGGAGACCGAGTGGCTCTTTGGCATGGATGAGGGCCGGAAACAGCTGGCGGCCA
  GTGCTGGCTTCAGGAGGTTGATTACAGTGGCCCTTCACCGAGGTCAGCAGTATGAAAGCA
  TGGACCACATCCAAGCTGAGCTGTCGGCTAGAGTCATGGAGCTGGCCCCAGCTGGGATGC
  CCACCCAGCAGCAGGTCCCCTTTCTGTCTGTGGGTGGGGACATTGGGGTCCGGACCGTTC
  AGCACCAAGACTGCAGCCCCTTGAGCGGTGACTATGTCATTGAGGATGTGCAAGGGGATG
  ACAAGCGATACTTCCGTCGACTGATCTTCCTCAGCAACAGGAATGTGGTGCAGTCCGAAG
  CCAGGTTGCTGAAGGATGTGTCTCACAAAGCCCAGAAGAAGCGGAAAAAGGACAGGAAGA
  AGCAGCGGCCTGCTGATGCGGAGGACCTCCCTGCAGCCCCGGGGCAGTCCATTGATAAGA
  GTTACCTGTGTTGTGAACACCACAAAGCCATGATCGCTGGCCTTGCCCTGCTGAGAAACC
  CAGAGCTACTCCTAGAGATCCCACTGGCATTGTTGGTGGTAGGCCTGGGCGGGGGCAGCC
  TCCCCCTCTTTGTCCACGATCATTTTCCAAAGTCCTGCATTGATGCTGTGGAGATCGATC
  CCTCCATGTTGGAAGTGGCCACCCAGTGGTTTGGCTTCTCCCAGAGTGACCGAATGAAGG
  TCCACATTGCAGATGGCCTGGACTATATCGCCAGCTTGGCAGGAGGAGGAGAAGCACGGC
  CTTGCTACGATGTCATAATGTTTGATGTTGACAGTAAGGACCCAACACTGGGAATGAGTT
  GTCCGCCCCCAGCATTTGTGGAGCAATCTTTTCTACAGAAGGTTAAAAGCATCTTGACTC
  CTGAAGGTGTTTTTATTCTCAACCTTGTGTGCCGAGACTTGGGGCTAAAAGACTCAGTGC
  TGGCTGGGCTCAAGGCAGTGTTCCCCCTCCTATATGTCCGGCGAATTGAGGGTGAAGTGA
  ATGAGATCCTGTTCTGTCAGCTGCACCCTGAGCAAAAACTTGCCACACCAGAGCTCCTAG
  AAACAGCCCAGGCTTTGGAGCGGACCCTGAGGAAGCCTGGGAGGGGTTGGGATGACACGT
  ATGTCTTGTCAGATATGCTCAAGACGGTGAAAATTGTGTGACTGCTTAGGCCAAGCAGCC
  CTCCTGCCTAGACTGACCTTGGACTCCCAGCCTGCCAGAGAATGAAGAAATACAACGCCC
  AGTACTTTTGAAGCTTCGTATTTTTCTTGGTTTCACACTCAGCTACATGTGACCTCCAGC
  TTGGTGAGGTTGCCTGAAGATTAGGGAAAATAAAAATGTCCTTCCCAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAAA
• Restriction Sites: ECoRI-NOT
• ACCN: NM_014955
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014955.2, NP_055770.1
• RefSeq Size: 3096 bp
• RefSeq ORF: 1842 bp
• Locus ID: 51603
• UniProt ID: Q8N6R0
• Cytogenetics: 1q24.3
• Protein Families: Druggable Genome
• Gene Summary: Dual methyltransferase that catalyzes methylation of elongation factor 1-alpha (EEF1A1 and EEF1A2) at two different positions, and is therefore involved in the regulation of mRNA translation (PubMed:30612740, PubMed:30143613). Via its C-terminus, methylates EEF1A1 and EEF1A2 at the N-terminal residue 'Gly-2' (PubMed:30143613). Via its N-terminus dimethylates EEF1A1 and EEF1A2 at residue 'Lys-55' (PubMed:30612740, PubMed:30143613). Has no activity towards core histones H2A, H2B, H3 and H4 (PubMed:30612740).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks an internal segment in the 5' region and uses a downstream AUG start codon, as compared to variant 1. The encoded isoform 2 has a shorter N-terminus, as compared to isoform 1.