P2Y10 (P2RY10) (NM_014499) Human Untagged Clone

CAT#: SC323852

P2RY10 (untagged)-Human purinergic receptor P2Y, G-protein coupled, 10 (P2RY10), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: P2Y10
• Synonyms: LYPSR2; P2Y10
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_014499.2 TGCCGCCAGAGTAAAGCTTTCTACCCTTTACTCCCTGCAAAGAAACAAGAGTGCTTATCC
  CAGCTAAGCTCCAGGGTAATGTTATCATGACAGCTTCAACTTTTAGACCACAGGCAAATG
  CTTTGTTAAAACTCTATGCTGGTCATTCCCTTCAGGATTTGGCACTCACCAACATACCCT
  TCTTTCAAGTGAAAAGGCATCTCTTTTAATGGTCCTGACCTTTGGAATAGGAAGCATGTA
  CCCTGGACAGAGCACTTCAAACTAGAGGAACCATAAATCCATGGCTAACCTTGACAAATA
  CACTGAAACATTCAAGATGGGTAGCAACAGTACCAGCACTGCTGAGATTTACTGTAATGT
  CACTAATGTGAAATTTCAATACTCCCTCTATGCAACCACCTATATCCTCATATTCATTCC
  TGGTCTTCTGGCTAACAGTGCAGCCTTGTGGGTTCTGTGCCGCTTCATCAGCAAGAAAAA
  TAAAGCCATCATTTTCATGATCAACCTCTCTGTGGCTGACCTTGCTCATGTATTATCTTT
  ACCCCTCCGGATTTACTATTACATCAGCCACCACTGGCCTTTCCAGAGAGCCCTTTGCCT
  GCTCTGCTTCTACCTGAAGTATCTCAACATGTATGCCAGCATTTGTTTCCTGACGTGCAT
  CAGTCTTCAAAGGTGCTTTTTTCTCCTCAAGCCCTTCAGGGCCAGAGACTGGAAGCGTAG
  GTACGATGTGGGCATCAGTGCTGCCATCTGGATCGTTGTGGGGACTGCCTGTTTGCCATT
  TCCCATCCTGAGAAGCACAGACTTAAACAACAACAAGTCCTGCTTTGCTGATCTTGGATA
  CAAGCAAATGAATGCAGTTGCGTTGGTCGGGATGATTACAGTTGCTGAGCTTGCAGGATT
  TGTGATCCCAGTGATCATCATCGCATGGTGTACCTGGAAAACTACTATATCCTTGAGACA
  GCCACCAATGGCTTTCCAAGGGATCAGTGAGAGGCAGAAAGCACTGCGGATGGTGTTCAT
  GTGTGCTGCAGTCTTCTTCATCTGCTTCACTCCCTATCATATTAACTTTATTTTTTACAC
  CATGGTAAAGGAAACCATCATTAGCAGTTGTCCCGTTGTCCGAATCGCACTGTATTTCCA
  CCCTTTTTGCCTGTGCCTTGCAAGTCTCTGCTGCCTTTTGGATCCAATTCTTTATTACTT
  TATGGCTTCAGAGTTTCGTGACCAACTATCCCGCCATGGCAGTTCTGTGACCCGCTCCCG
  CCTCATGAGCAAGGAGAGTGGTTCATCAATGATTGGCTAAAATTAAGATATCTCTTTAAT
  TACGCCTTTGTTTACCTACGTTCCTTGTCTTTTTCCAAAGGCCAGAATTGTCAACCAATT
  TCTTTAATTGAACATTGTAAAAAACAGGAATAAGTACTTTTGTGTAATATTCACAGTCAA
  CAGGGGTGTGATGGTGAAGGCAGAGTGTGAAAAACGTGAGAGAGGAAGAGAAAATAGATT
  TACCTGATTCCTCTTTAAAATTCAAGCCACTTTCTTATTTAAGAAACCTAGATCAAGTTT
  TTACAGATGTAAATAAAAGTTGAATAGTTTACCTTAAATTTTTTTCAATAAGTAAGTTAT
  TGTTAATAATGCACAGTAAATATGTGAATTTTTCCTAGATGTAAAAAAAAAAAATCTTTC
  ATATAAAGACCTTAAATTCTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: ECoRI-NOT
• ACCN: NM_014499
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014499.2, NP_055314.1
• RefSeq Size: 1871 bp
• RefSeq ORF: 1020 bp
• Locus ID: 27334
• UniProt ID: O00398
• Cytogenetics: Xq21.1
• Domains: 7tm_1
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction
• Gene Summary: The protein encoded by this gene belongs to the family of G-protein coupled receptors that are preferentially activated by adenosine and uridine nucleotides. There is a pseudogene for this gene nearby on chromosome X. Multiple alternatively spliced transcripts have been observed. [provided by RefSeq, Apr 2016]
  Transcript Variant: This variant (1) represents the longest transcript. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.