VPS33B (NM_018668) Human Untagged Clone

CAT#: SC323727

VPS33B (untagged)-Human vacuolar protein sorting 33 homolog B (yeast) (VPS33B)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: VPS33B
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_018668.3 GACAAGGCTGCTCCGAGGTAGTAGCTCTCTTGCCTGGAGGTGGCCATTCATTCCTGGAGT
  GCTGCTGAGGAGCGAGGGCCCATCTGGGGTCTCTGGAAGTCGGTGCCCAGGCCTGAAGGA
  TAGCCCCCCTTGCGCTTCCCTGGGCTGCGGCCGGCCTTCTCAGAACGAAGGGCGTCCTTC
  CACCCCGCGGCGCAGGTGATCGCTGCCATGGCTTTTCCCCATCGGCCGGACGCCCCTGAG
  CTGCCTGACTTCTCCATGCTGAAGAGGCTGGCTCGAGACCAGCTCATCTATCTGCTGGAG
  CAGCTTCCTGGAAAAAAGGATTTATTCATTGAGGCAGATCTCATGAGCCCTTTGGATCGA
  ATTGCCAATGTCTCCATCCTGAAGCAACACGAAGTAGACAAGCTATACAAGGTGGAGAAC
  AAGCCAGCCCTCAGCTCCAATGAACAATTGTGCTTCTTGGTCAGACCCCGCATCAAGAAT
  ATGCGATACATTGCCAGTCTTGTCAATGCTGACAAATTGGCTGGCCGAACTCGCAAATAC
  AAAGTGATCTTCAGCCCTCAAAAGTTCTATGCGTGTGAGATGGTGCTTGAGGAAGAGGGA
  ATCTATGGAGATGTGAGCTGTGATGAATGGGCCTTCTCTTTGCTGCCTCTTGATGTGGAT
  CTGCTGAGCATGGAACTACCAGAATTTTTCAGGGATTACTTTCTGGAAGGAGATCAGCGT
  TGGATCAACACTGTAGCTCAGGCCTTACACCTTCTCAGCACTCTCTATGGACCCTTTCCA
  AACTGCTATGGAATTGGCAGGTGCGCCAAGATGGCATATGAATTGTGGAGGAACCTGGAG
  GAGGAGGAGGATGGCGAAACCAAGGGCCGAAGGCCAGAGATTGGACATATCTTTCTCTTG
  GACAGAGATGTGGACTTTGTGACAGCACTTTGCTCCCAAGTGGTTTATGAGGGCCTAGTA
  GATGACACCTTCCGCATCAAGTGTGGGAGTGTCGACTTTGGCCCAGAAGTCACATCCTCT
  GACAAGAGCCTGAAGGTGCTACTCAATGCCGAGGACAAGGTGTTTAATGAGATTCGGAAC
  GAGCACTTCTCCAATGTCTTTGGCTTCTTGAGCCAGAAGGCCCGGAACTTGCAGGCCCAG
  TATGATCGCCGGAGAGGCATGGACATTAAGCAGATGAAGAATTTCGTGTCCCAGGAGCTC
  AAGGGCCTGAAACAGGAGCACCGCCTGCTGAGTCTCCATATTGGGGCCTGTGAATCCATC
  ATGAAGAAGAAAACCAAGCAGGATTTCCAGGAGCTAATCAAGACTGAGCATGCACTGCTA
  GAGGGGTTCAACATCCGGGAGAGCACCAGCTACATTGAGGAACACATAGACCGGCAGGTG
  TCGCCTATAGAAAGCCTGCGCCTCATGTGCCTTTTGTCCATCACTGAGAATGGTTTGATC
  CCCAAGGATTACCGATCTCTGAAAACACAGTATCTGCAGAGCTATGGCCCTGAGCACCTG
  CTAACCTTCTCCAATCTGCGAAGAGCTGGGCTCCTAACGGAGCAGGCCCCCGGGGACACC
  CTCACAGCCGTGGAGAGTAAAGTGAGCAAGCTGGTGACCGACAAGGCTGCAGGAAAGATT
  ACTGATGCCTTCAGTTCTCTGGCCAAGAGGAGCAATTTTCGTGCCATCAGCAAAAAGCTG
  AATTTGATCCCACGTGTGGACGGCGAGTATGATCTGAAAGTGCCCCGAGACATGGCTTAC
  GTCTTCAGTGGTGCTTATGTGCCCCTGAGCTGCCGAATCATTGAGCAGGTGCTAGAGCGG
  CGAAGCTGGCAGGGCCTTGATGAGGTGGTACGGCTGCTCAACTGCAGTGACTTTGCATTC
  ACAGATATGACTAAGGAAGACAAGGCTTCCAGTGAGTCCCTGCGCCTCATCTTGGTGGTG
  TTCTTGGGTGGTTGTACATTCTCTGAGATCTCAGCCCTCCGGTTCCTGGGCAGAGAGAAA
  GGCTACAGGTTCATTTTCCTGACGACAGCAGTCACAAACAGCGCTCGCCTTATGGAGGCC
  ATGAGTGAGGTGAAAGCCTGATGTTTTTCCCGGCCAGTGTTGACATCTTCCCTGAACACA
  TTCCTCAGTGAGATGCAGGCATCTGGCACCCAGCTGCTATAACCAAGTGTCCACCAACTA
  CCTGCTAAGAGCCGGGAGCATGGAACGTGTTGGGATTTAGAGAACATTATCTGAGAAAAG
  AGTTCACTTCCTGCTCCCAGGATATTTCTCTTTTCTGTTTATGAAGTACAACCCATGCTG
  CTAAGATGCGAGCAGGAAGAGGCATCCTTTGCTAAATCCTGTTTGAATGTCATTGTAAAT
  AAAGCCTCTGCTCTCAGATGTAAAAAAAAAAAAAAA
• Restriction Sites: ECoRI-NOT
• ACCN: NM_018668
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018668.3, NP_061138.3
• RefSeq Size: 2639 bp
• RefSeq ORF: 1854 bp
• Locus ID: 26276
• UniProt ID: Q9H267
• Cytogenetics: 15q26.1
• Domains: Sec1
• Gene Summary: Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Genetic studies in yeast have identified more than 40 vacuolar protein sorting (VPS) genes involved in vesicle transport to vacuoles. This gene is a member of the Sec-1 domain family, and encodes the human ortholog of rat Vps33b which is homologous to the yeast class C Vps33 protein. The mammalian class C vacuolar protein sorting proteins are predominantly associated with late endosomes/lysosomes, and like their yeast counterparts, may mediate vesicle trafficking steps in the endosome/lysosome pathway. Mutations in this gene are associated with arthrogryposis-renal dysfunction-cholestasis syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).