NUP133 (NM_018230) Human Untagged Clone

CAT#: SC322454

NUP133 (untagged)-Human nucleoporin 133kDa (NUP133)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NUP133
• Synonyms: GAMOS8; hNUP133; NPHS18
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for SC322454 TTAAGTTCCGCGCGCAGGCCAGGCTGCAACCTGACGGCCAGATCCCTCGCTGTCCTAGTC
  GCTGCTCCTTGGAGTCATGTTCCCAGCCGCCCCTTCTCCGCGGACCCCGGGTACCGGGTC
  CCGAAGGGGCCCGCTGGCCGGACTCGGGCCCGGCTCCACGCCCCGGACGGCTAGCAGGAA
  GGGTCTGCCCCTGGGGTCTGCAGTCAGCTCCCCAGTGCTCTTCTCGCCGGTCGGCCGGCG
  TAGCTCGCTAAGCTCGCGGGGAACACCAACACGAATGTTCCCACACCACTCCATAACTGA
  GTCTGTGAACTATGATGTGAAAACGTTTGGATCTTCTCTTCCTGTTAAAGTCATGGAAGC
  CCTAACATTGGCTGAAGTCGATGACCAGCTGACCATTAACATAGATGAAGGTGGATGGGC
  TTGTCTGGTGTGCAAAGAGAAGCTCATTATTTGGAAGATTGCTCTGTCACCTATTACTAA
  GTTATCCGTTTGCAAAGAACTTCAGCTGCCACCTAGTGATTTCCACTGGAGTGCCGACTT
  AGTGGCTCTTTCTTACTCTTCTCCCTCAGGTGAAGCACATTCTACTCAGGCTGTTGCTGT
  CATGGTTGCCACCAGAGAAGGATCTATCCGCTATTGGCCAAGCCTTGCTGGTGAAGATAC
  CTACACAGAGGCTTTTGTAGATTCGGGAGGTGATAAGACTTACAGTTTCCTAACAGCAGT
  GCAGGGAGGAAGTTTTATTTTGTCTTCATCAGGAAGCCAACTAATTCGGTTGATACCTGA
  GAGCTCAGGAAAGATTCATCAGCATATCCTGCCTCAGGGGCAAGGCATGCTTTCAGGAAT
  TGGTCGAAAAGTTTCTTCTCTTTTTGGAATTTTATCTCCTAGTAGTGATCTCACACTTTC
  AAGTGTTCTCTGGGATAGAGAGAGATCAAGCTTTTATAGCCTGACGAGTTCAAACATCAG
  TAAATGGGAATTAGATGATTCTTCAGAAAAGCATGCATACAGTTGGGATATAAATAGAGC
  CCTGAAGGAAAACATTACCGATGCTATTTGGGGATCTGAAAGTAACTATGAAGCTATTAA
  AGAAGGAGTCAACATTCGATATTTGGACTTTAAGCAAAACTGTGATGGGCTGGTGATTTT
  GGCAGCAGCATGGCACTCAGCAGACAATCCATGTCTCATCTATTACTCTCTGATAACAAT
  AGAAGATAATGGTTGCCAAATGTCAGATGCAGTTACTGTAGAAGTCACTCAATATAATCC
  ACCTTTTCAGTCTGAAGACCTGATTTTGTGTCAGTTGACGGTCCCAAACTTTTCAAACCA
  GACTGCCTATCTGTATAACGAAAGTGCTGTCTATGTGTGCTCCACAGGAACTGGGAAATT
  TTCTCTTCCCCAGGAGAAAATTGTCTTTAATGCACAAGGAGATAGTGTTTTAGGTGCTGG
  TGCCTGTGGTGGTGTTCCTATCATTTTTTCTAGAAACAGTGGACTGGTGTCTATTACTTC
  AAGGGAAAATGTGTCTATATTGGCAGAAGACTTGGAAGGGTCTTTAGCATCTTCAGTTGC
  TGGACCAAACAGTGAGAGTATGATTTTTGAGACCACTACAAAGAATGAAACTATAGCCCA
  GGAAGATAAAATCAAGTTGCTGAAAGCTGCCTTTCTGCAATACTGCAGAAAAGATTTAGG
  TCATGCTCAAATGGTGGTTGATGAGCTCTTTTCCTCTCACTCTGATTTGGATTCTGATTC
  TGAACTAGACAGGGCAGTTACCCAAATCAGTGTAGACCTGATGGATGACTACCCAGCATC
  TGACCCACGGTGGGCTGAGTCTGTCCCTGAGGAAGCACCTGGGTTCAGCAATACGTCACT
  GATTATCCTTCACCAGCTAGAAGACAAGATGAAAGCTCACTCTTTTCTTATGGACTTTAT
  TCATCAAGTTGGCTTATTTGGACGTCTAGGCAGTTTTCCAGTTAGAGGGACACCGATGGC
  CACTCGACTGTTGCTCTGTGAGCATGCCGAAAAGCTGTCAGCCGCCATTGTTCTCAAGAA
  CCACCACTCCCGGCTTTCTGACCTTGTCAACACAGCCATATTGATTGCTTTGAACAAGAG
  GGAGTATGAAATCCCATCCAACCTGACTCCTGCAGATGTCTTTTTCAGGGAGGTATCCCA
  AGTAGATACCATCTGTGAGTGCTTACTGGAGCATGAGGAGCAAGTCTTGAGGGATGCACC
  TATGGATTCCATTGAATGGGCTGAAGTGGTGATCAATGTGAACAATATTCTCAAGGATAT
  GCTGCAGGCTGCTAGTCATTATCGCCAAAATAGAAACTCTTTGTATAGAAGAGAAGAATC
  ACTAGAAAAAGAACCTGAATATGTTCCATGGACGGCAACAAGTGGTCCTGGTGGCATCCG
  AACGGTAATAATACGCCAGCATGAGATTGTCCTGAAGGTGGCTTATCCACAGGCAGACAG
  CAACCTCCGAAACATCGTGACCGAGCAGCTGGTAGCCCTGATCGATTGCTTCCTGGATGG
  TTATGTTTCTCAGCTTAAGTCTGTGGATAAATCCAGTAATCGGGAAAGATATGACAATCT
  GGAGATGGAATACCTACAGAAAAGATCAGATCTCTTATCTCCTCTTCTTTCACTAGGCCA
  GTACCTGTGGGCTGCTTCTCTAGCAGAGAAATACTGTGACTTTGATATATTGGTACAAAT
  GTGTGAGCAGACTGACAACCAGAGCCGACTCCAGCGCTACATGACCCAGTTTGCTGATCA
  GAATTTTTCAGACTTTCTCTTCCGTTGGTATCTGGAGAAAGGAAAGCGAGGCAAATTATT
  ATCTCAGCCCATTTCTCAGCATGGACAGTTGGCAAATTTTTTGCAAGCTCATGAACATCT
  CAGCTGGTTACATGAAATTAATAGCCAAGAATTAGAAAAGGCTCATGCAACACTTCTGGG
  TTTGGCAAATATGGAAACTCGTTACTTTGCAAAGAAGAAAACCCTTCTTGGCTTGAGTAA
  ATTGGCTGCATTAGCTTCAGACTTTTCAGAGGATATGCTACAAGAAAAAATTGAAGAAAT
  GGCTGAGCAGGAGCGCTTTCTACTGCATCAGGAGACCCTACCTGAACAGCTGCTGGCGGA
  GAAACAGCTAAATCTCAGTGCGATGCCAGTATTGACTGCACCACAACTCATTGGTCTATA
  TATCTGTGAAGAAAATAGAAGAGCTAATGAATATGATTTCAAGAAAGCTTTGGACTTGTT
  GGAATATATTGATGAGGAAGAAGATATAAATATAAATGATCTAAAACTGGAAATCCTTTG
  CAAAGCTCTTCAGAGAGATAACTGGTCCAGTTCTGATGGCAAAGATGATCCAATTGAAGT
  ATCTAAAGACAGTATATTTGTGAAGATCTTACAGAAACTTTTAAAAGATGGCATTCAGCT
  CAGTGAGTACTTACCGGAGGTGAAAGACCTGCTACAAGCGGATCAGCTTGGAAGCTTAAA
  GTCCAATCCTTACTTCGAGTTTGTTTTGAAAGCAAATTATGAATATTATGTTCAGGGACA
  AATATAACTTTTTCTAAAAATGGCCATTGTTTATGAAATCTGTATAAGTGTTTCCTTATA
  CAAATTTTAGGCCATAAACAAGTGTAAGTTTGTACAATTTCATAACATGTATAGCTGAGT
  TTTTATACTTTATATGTAGGAAGCTAATATAAAATAGTTATGTAACTGTGAAAAAAAAAA
  AAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_018230
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018230.2, NP_060700.2
• RefSeq Size: 4170 bp
• RefSeq ORF: 3471 bp
• Locus ID: 55746
• UniProt ID: Q8WUM0
• Cytogenetics: 1q42.13
• Domains: Nup133
• Gene Summary: The nuclear envelope creates distinct nuclear and cytoplasmic compartments in eukaryotic cells. It consists of two concentric membranes perforated by nuclear pores, large protein complexes that form aqueous channels to regulate the flow of macromolecules between the nucleus and the cytoplasm. These complexes are composed of at least 100 different polypeptide subunits, many of which belong to the nucleoporin family. The nucleoporin protein encoded by this gene displays evolutionarily conserved interactions with other nucleoporins. This protein, which localizes to both sides of the nuclear pore complex at interphase, remains associated with the complex during mitosis and is targeted at early stages to the reforming nuclear envelope. This protein also localizes to kinetochores of mitotic cells. [provided by RefSeq, Jul 2008]