DDAH1 (NM_012137) Human Untagged Clone

CAT#: SC321756

DDAH1 (untagged)-Human dimethylarginine dimethylaminohydrolase 1 (DDAH1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DDAH1
• Synonyms: DDAH; DDAH-1; DDAHI; HEL-S-16
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_012137.2 CAAGAGGAGCCGACGGGCGCTCGCAGGCTCAGCGCGCGCTGCCCGCGGCAGGACCCGGCC
  GCCTCCGCCGCCGCCGCCGCCCCTAAGCCTCCCGAAGCCATGGCCGGGCTCGGCCACCCC
  GCCGCCTTCGGCCGGGCCACCCACGCCGTGGTGCGGGCGCTACCCGAGTCGCTCGGCCAG
  CACGCGCTGAGAAGCGCCAAGGGCGAGGAGGTGGACGTCGCCCGCGCGGAACGGCAGCAC
  CAGCTCTACGTGGGCGTGCTGGGCAGCAAGCTGGGGCTGCAGGTGGTGGAGCTGCCGGCC
  GACGAGAGCCTTCCGGACTGCGTCTTCGTGGAGGACGTGGCCGTGGTGTGCGAGGAGACG
  GCCCTCATCACCCGACCCGGGGCGCCGAGCCGGAGGAAGGAGGTTGACATGATGAAAGAA
  GCATTAGAAAAACTTCAGCTCAATATAGTAGAGATGAAAGATGAAAATGCAACTTTAGAT
  GGCGGAGATGTTTTATTCACAGGCAGAGAATTTTTTGTGGGCCTTTCCAAAAGGACAAAT
  CAACGAGGTGCTGAAATCTTGGCTGATACTTTTAAGGACTATGCAGTCTCCACAGTGCCA
  GTGGCAGATGGGTTGCATTTGAAGAGTTTCTGCAGCATGGCTGGGCCTAACCTGATCGCA
  ATTGGGTCTAGTGAATCTGCACAGAAGGCCCTTAAGATCATGCAACAGATGAGTGACCAC
  CGCTACGACAAACTCACTGTGCCTGATGACATAGCAGCAAACTGTATATATCTAAATATC
  CCCAACAAAGGGCACGTCTTGCTGCACCGAACCCCGGAAGAGTATCCAGAAAGTGCAAAG
  GTTTATGAGAAACTGAAGGACCATATGCTGATCCCCGTGAGCATGTCTGAACTGGAAAAG
  GTGGATGGGCTGCTCACCTGCTGCTCAGTTTTAATTAACAAGAAAGTAGACTCCTGAGCT
  GCAGAGTCCCCCCCGGTAGCCGGCAAGACCGCACAGGCAAGGCCGATGACTCTGTGCCCA
  CTCCTGTTGTTTTCCTTGACAATCTGATTCAATTTAGTTAAAGGATGATATAGAGAAAAT
  ACATTATTTAGCATTATTTCTTCAGCTATAATGAATTGCTATAGAAATCAGGCAGATCTT
  TCTAATGTGTATTGATTGGTCTTTTCAGCTACTCTGAACAGATTACTAAGGCCATCTCCT
  CATCTCTAAGGGAGAAAAATAGTCTGTAGATGAATAATGTAAGGTAAAGAGTTGCATGTC
  AGTCTTTGTAATTATTTACACTTTAACTTTCTCCAGAACTCAGACATGATTTCAACATGG
  TGTTAGATTTGTGCATTTTATTTTCCTGACCACCTCATTCCAGCCAATGTATGGTTATCC
  ACTCTGTGTGCCAAAACCAATCATGCCTTTCACGGCCCTTTAGTTCAGAGAAGTTCTGCA
  CTGATTTTTAGTCTCTTGATGTCTCAATCTTACATGTATACCAATCACAATGGAATAAAG
  TGTTGAGTTGTACTGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAA
• Restriction Sites: Please inquire
• ACCN: NM_012137
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012137.2, NP_036269.1
• RefSeq Size: 3949 bp
• RefSeq ORF: 858 bp
• Locus ID: 23576
• UniProt ID: O94760
• Cytogenetics: 1p22.3
• Domains: Amidinotransf
• Gene Summary: This gene belongs to the dimethylarginine dimethylaminohydrolase (DDAH) gene family. The encoded enzyme plays a role in nitric oxide generation by regulating cellular concentrations of methylarginines, which in turn inhibit nitric oxide synthase activity. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) encodes the longest isoform (1).