NFIC (NM_005597) Human Untagged Clone

CAT#: SC321627

NFIC (untagged)-Human nuclear factor I/C (CCAAT-binding transcription factor) (NFIC), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NFIC
• Synonyms: CTF; CTF5; NF-I; NFI
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_005597.2 AATGACTCAGTAAGTTCAGCGCGCCCGCTCCGGCCGGCCCTGCGCCTCCCGCCGCGCCCG
  GGATGTATTCGTCCCCGCTCTGCCTCACCCAGGATGAGTTCCACCCGTTCATCGAGGCCC
  TGCTGCCTCACGTCCGCGCCTTCGCCTACACCTGGTTCAACCTGCAGGCGCGGAAGCGCA
  AGTACTTCAAGAAGCACGAGAAGCGGATGTCGAAGGACGAGGAGCGTGCGGTCAAGGACG
  AGCTGCTGGGCGAGAAGCCCGAGGTCAAGCAGAAGTGGGCGTCGCGGCTGCTGGCCAAGC
  TGCGCAAGGACATCCGGCCCGAGTGCCGCGAGGACTTCGTGCTGAGCATCACCGGCAAGA
  AGGCGCCGGGCTGCGTGCTCTCCAACCCCGACCAGAAGGGCAAGATGCGGCGCATCGACT
  GTCTCCGGCAGGCGGACAAGGTGTGGCGGCTGGACCTGGTCATGGTCATCCTGTTCAAGG
  GCATCCCGCTGGAGAGCACCGACGGCGAGCGCCTGGTCAAGGCTGCGCAGTGCGGTCACC
  CGGTCCTGTGCGTGCAGCCGCACCACATTGGCGTGGCCGTCAAGGAGCTGGACCTCTACC
  TGGCCTACTTCGTGCGTGAGCGAGATGCAGAGCAAAGCGGCAGTCCCCGGACAGGGATGG
  GCTCTGACCAGGAGGACAGCAAGCCCATCACGCTGGACACGACCGACTTCCAGGAGAGCT
  TTGTCACCTCCGGCGTGTTCAGCGTCACTGAGCTCATCCAAGTGTCCCGGACACCCGTGG
  TGACTGGAACAGGACCCAACTTCTCCCTGGGGGAGCTGCAGGGGCACCTGGCATACGACC
  TGAACCCAGCCAGCACTGGCCTCAGAAGAACGCTGCCCAGCACCTCCTCCAGTGGGAGCA
  AGCGGCACAAATCGGGCTCGATGGAGGAAGACGTGGACACGAGCCCTGGCGGCGATTACT
  ACACTTCGCCCAGCTCGCCCACGAGTAGCAGCCGCAACTGGACGGAGGACATGGAAGGAG
  GCATCTCGTCCCCGGTGAAGAAGACAGAGATGGACAAGTCACCATTCAACAGCCCGTCCC
  CCCAGGACTCTCCCCGCCTCTCCAGCTTCACCCAGCACCACCGGCCCGTCATCGCCGTGC
  ACAGCGGGATCGCCCGGAGCCCACACCCGTCCTCCGCTCTGCATTTCCCTACGACGTCCA
  TCCTACCCCAGACGGCCTCCACCTACTTCCCCCACACGGCCATCCGCTACCCACCTCATC
  TCAACCCCCAGGACCCGCTCAAAGATCTTGTCTCGCTGGCCTGCGACCCAGCCAGCCAGC
  AACCTGGACCGTCCTGGTATCTGGGATAGCAAAGGTCTTCTTCCCTCGCCCCTTCTCCAT
  CGTCCCAGGAATCCCAGGGGGCAGCACAGCCGGCCCCCGGCCCACGTTTTCGGTGGAAAA
  TTAGAGTGAACAAGAACACCCCTGCCGACTCCCAGCCCGGCCAAAAAGACAAAACACATA
  GACGCACACACTCAGGAGGAAAAGAAAAAACAAAGGCAGAAGAAGAAAAAAAAAAAAAAA
  AAA
• Restriction Sites: Please inquire
• ACCN: NM_005597
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.

Reference Data

• RefSeq: NM_005597.2, NP_005588.2
• RefSeq Size: 2185 bp
• RefSeq ORF: 1287 bp
• Locus ID: 4782
• UniProt ID: P08651
• Cytogenetics: 19p13.3
• Protein Families: Transcription Factors
• Gene Summary: The protein encoded by this gene belongs to the CTF/NF-I family. These are dimeric DNA-binding proteins, and function as cellular transcription factors and as replication factors for adenovirus DNA replication. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Oct 2011]
  Transcript Variant: This variant (5) lacks two consecutive in-frame coding exons compared to variant 1. This results in a shorter isoform (5) missing an internal protein segment compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.