WDR24 (NM_032259) Human Untagged Clone

CAT#: SC321280

WDR24 (untagged)-Human WD repeat domain 24 (WDR24)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: WDR24
• Synonyms: C16orf21; JFP7
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_032259.1 GGAAGTCCCCTCACCGCTCAGCCAGGGTGATGGGCTGGACTGACAGACTCCAGTGAATTT
  GAGCTTGCCTGTCAGGCTGATTGGCTGATAGACAGCCCTGGATTGGCTCACTAAGACTGA
  CCAGCCCGGGACCAAGCAGTTCTGGGGTCCCAACCTGGGTGGAAGGTCTGAACTGATGAC
  CCACCCAGGCTGACCAGGCCAGCCCACCTCACTGACCTCCTGACCCCTGACCTCATCACC
  TGTGCAGCCATGGAGAAGATGTCCCGTGTGACCACAGCCCTGGGTGGCAGCGTGCTGACA
  GGCCGCACCATGCACTGCCACCTGGATGCTCCCGCCAATGCCATCAGTGTGTGCCGCGAC
  GCAGCCCAGGTGGTCGTGGCAGGCCGTAGCATCTTCAAGATCTATGCCATCGAGGAGGAA
  CAGTTCGTGGAAAAGCTGAACCTGCGTGTGGGGCGCAAGCCTTCGCTTAACCTGAGCTGT
  GCTGACGTGGTCTGGCACCAGATGGATGAGAACCTGCTGGCCACAGCAGCCACCAATGGC
  GTGGTGGTCACGTGGAACCTGGGCCGGCCATCCCGCAACAAGCAGGACCAGCTGTTCACA
  GAACACAAGCGCACGGTAAACAAAGTCTGCTTCCACCCCACCGAAGCCCACGTGCTGCTC
  AGTGGCTCCCAGGATGGCTTCATGAAGTGCTTTGACCTCCGCAGAAAGGACTCTGTCAGC
  ACCTTCTCGGGCCAGTCGGAGAGCGTGCGGGACGTGCAGTTCAGTATCCGGGACTACTTC
  ACCTTCGCCTCCACCTTTGAGAACGGCAATGTGCAGCTCTGGGACATCCGGCGTCCCGAC
  CGGTGCGAGAGGATGTTCACAGCCCACAACGGACCCGTCTTCTGCTGCGACTGGCACCCC
  GAGGACAGGGGCTGGTTGGCCACTGGAGGGCGCGACAAGATGGTGAAGGTCTGGGACATG
  ACCACGCACCGTGCCAAGGAGATGCACTGTGTGCAGACCATCGCCTCGGTGGCCCGTGTG
  AAGTGGCGGCCAGAGTGCCGCCACCACCTGGCCACATGCTCCATGATGGTGGACCACAAC
  ATCTATGTTTGGGACGTGCGCCGGCCCTTCGTGCCAGCTGCCATGTTTGAGGAACACCGA
  GACGTCACCACGGGAATTGCCTGGCGCCACCCCCACGACCCCTCCTTCCTGCTGTCTGGC
  TCCAAGGACAGCTCGCTGTGCCAGCACCTGTTCCGCGACGCCAGCCAGCCCGTCGAGCGC
  GCCAACCCTGAGGGCCTCTGCTACGGCCTCTTCGGGGACCTGGCCTTCGCCGCCAAGGAG
  AGCCTCGTGGCTGCCGAGTCGGGGCGCAAGCCCTACACTGGCGACCGGCGCCACCCCATC
  TTCTTTAAGCGCAAGCTGGACCCTGCCGAGCCCTTCGCAGGCCTCGCCTCCAGTGCCCTC
  AGTGTCTTTGAGACGGAGCCAGGTGGCGGCGGCATGCGCTGGTTTGTGGACACAGCTGAG
  CGTTATGCGCTGGCTGGCCGGCCACTGGCCGAGCTCTGTGACCACAACGCAAAGGTGGCT
  CGAGAGCTTGGCCGCAACCAGGTGGCGCAAACGTGGACCATGCTGCGGATCATCTACTGC
  AGCCCTGGCCTAGTGCCCACTGCAAACCTCAACCACAGTGTGGGCAAGGGTGGCTCCTGT
  GGCCTCCCGCTCATGAACAGTTTCAACCTGAAGGATATGGCCCCAGGGTTGGGCAGTGAG
  ACGCGGCTGGACCGCAGCAAAGGAGATGCACGGAGCGACACAGTTCTGCTCGACTCCTCG
  GCCACACTCATCACCAATGAGGATAACGAGGAAACCGAGGGCAGCGACGTACCTGCCGAC
  TACCTGCTGGGTGACGTGGAAGGTGAGGAGGACGAGCTGTACCTGCTGGATCCGGAACAC
  GCGCACCCCGAGGACCCTGAGTGCGTGCTGCCGCAGGAGGCCTTTCCGCTGCGCCACGAG
  ATCGTGGACACGCCTCCCGGGCCCGAGCACCTGCAGGACAAGGCCGACTCCCCGCACGTG
  AGCGGCAGCGAGGCGGATGTGGCCTCCCTGGCCCCCGTGGACTCCTCCTTCTCGCTCCTG
  TCTGTCTCACACGCGCTCTACGACAGCCGCCTGCCGCCCGACTTCTTCGGCGTGCTGGTG
  CGCGACATGCTGCACTTCTACGCTGAGCAGGGCGACGTGCAGATGGCTGTGTCTGTGCTC
  ATCGTCCTGGGTGAACGGGTGCGCAAGGACATCGACGAGCAGACCCAGGAGCACTGGTAC
  ACTTCCTACATCGACCTGCTGCAGCGCTTCCGCCTCTGGAACGTGTCCAACGAGGTGGTC
  AAGCTGAGCACCAGCCGCGCCGTCAGCTGCCTCAACCAGGCCTCCACCACCCTGCACGTC
  AACTGCAGCCACTGCAAGCGGCCCATGAGCAGCCGGGGCTGGGTCTGCGACAGGTGCCAC
  CGCTGCGCCAGCATGTGTGCCGTCTGCCACCACGTAGTCAAGGGTCTCTTCGTGTGGTGC
  CAGGGCTGCAGCCACGGCGGCCACCTGCAGCACATCATGAAGTGGCTGGAAGGCAGCTCC
  CACTGTCCCGCAGGCTGCGGCCACCTCTGCGAGTACTCCTGACGGGGCATCTGCTGGGCT
  TGCCCGGGCGGCCGCGTGCAGAACCCCGCCTGGTCTTGTGCCCGAGACGGGCGGAGGCTG
  GAACTTGAGACCTCAATAAAGGAAGTAGAGCCGCAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC
• Restriction Sites: Please inquire
• ACCN: NM_032259
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032259.1, NP_115635.1
• RefSeq Size: 3166 bp
• RefSeq ORF: 3165 bp
• Locus ID: 84219
• UniProt ID: Q96S15
• Cytogenetics: 16p13.3
• Domains: WD40
• Protein Families: Druggable Genome
• Gene Summary: As a component of the GATOR subcomplex GATOR2, functions within the amino acid-sensing branch of the TORC1 signaling pathway (PubMed:23723238, PubMed:27166823). Indirectly activates mTORC1 and the TORC1 signaling pathway through the inhibition of the GATOR1 subcomplex (PubMed:23723238). It is negatively regulated by the upstream amino acid sensors SESN2 and CASTOR1 (PubMed:26449471, PubMed:26586190, PubMed:27487210). In addition to its role in regulation of the TORC1 complex, promotes the acidification of lysosomes and facilitates autophagic flux (PubMed:27166823).[UniProtKB/Swiss-Prot Function]