PELO (NM_015946) Human Untagged Clone

CAT#: SC320778

PELO (untagged)-Human pelota homolog (Drosophila) (PELO)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PELO
• Synonyms: CGI-17; PRO1770
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_015946.4 GCCCGCTGTTGCGTGCTGCCAGCGGGAACTGTGTAGGGGTAGATTTTCGCTGCAGTGTTC
  CCCGAGCCTGTTAGACGCAGCGCGCCGGGAGACTGAGAGAGGAAAGGATAGAGGAAGTGC
  TGCCCTAGGCTGCATGAGTCGAAGCAAGCGTGTTTCCTTCCCGCCAGGCAAGTGCCCTTA
  GAAACCGGGCCCCGCCCCCTTCCTGGCCTGCATTCCCATCCCCTCTCCCGGGGCGGAGGT
  GAGGACCTCTTTGGTTCCTTTGGTTCTGTCAGTGAGCCCCTTCCTTGGCCATGAAGCTCG
  TGAGGAAGAACATCGAGAAGGACAATGCGGGCCAGGTGACCCTGGTCCCCGAGGAGCCTG
  AGGACATGTGGCACACTTACAACCTCGTGCAGGTGGGCGACAGCCTGCGCGCCTCCACCA
  TCCGCAAGGTACAGACAGAGTCCTCCACGGGCAGCGTGGGCAGCAACCGGGTCCGCACTA
  CCCTCACTCTCTGCGTGGAGGCCATCGACTTCGACTCTCAAGCCTGCCAGCTGCGGGTTA
  AGGGGACCAACATCCAAGAGAATGAGTATGTCAAGATGGGGGCTTACCACACCATCGAGC
  TGGAGCCCAACCGCCAGTTCACCCTGGCCAAGAAGCAGTGGGATAGTGTGGTACTGGAGC
  GCATCGAGCAGGCCTGTGACCCAGCCTGGAGCGCTGATGTGGCGGCTGTGGTCATGCAGG
  AAGGCCTCGCCCATATCTGCTTAGTCACTCCCAGCATGACCCTCACTCGGGCCAAGGTGG
  AGGTGAACATCCCTAGGAAAAGGAAAGGCAATTGCTCTCAGCATGACCGGGCCTTGGAGC
  GGTTCTATGAACAGGTGGTCCAGGCTATCCAGCGCCACATACACTTTGATGTTGTAAAGT
  GCATCCTGGTGGCCAGCCCAGGATTTGTGAGGGAGCAGTTCTGCGACTACATGTTTCAAC
  AAGCAGTGAAGACCGACAACAAACTGCTCCTGGAAAACCGGTCCAAATTTCTTCAGGTAC
  ATGCCTCCTCCGGACACAAGTACTCCCTGAAAGAGGCCCTTTGTGACCCTACTGTGGCTA
  GCCGCCTTTCAGACACTAAAGCTGCTGGGGAAGTCAAAGCCTTGGATGACTTCTATAAAA
  TGTTACAGCATGAACCGGATCGAGCTTTCTATGGACTCAAGCAGGTGGAGAAGGCCAATG
  AAGCCATGGCAATTGACACATTGCTCATCAGCGATGAGCTCTTCAGGCATCAGGATGTAG
  CCACACGGAGCCGGTATGTGAGGCTGGTGGACAGTGTGAAAGAGAATGCAGGCACCGTTA
  GGATATTCTCTAGTCTTCACGTTTCTGGGGAACAGCTCAGCCAGTTGACTGGGGTAGCTG
  CCATTCTCCGCTTCCCTGTTCCCGAACTTTCTGACCAAGAGGGTGATTCCAGTTCTGAAG
  AGGATTAATGATTGAAACTTAAAATTGAGACAATCTTGTGTTTCCTAAACTGTTACAGTA
  CATTTCTCAGCATCCTTGTGACAGAAAGCTGCAAGAAGGGCACTTTTTGATTCATACAGG
  GATTTCTTATGTCTTTGGCTACACTAGATATTTTGTGATTGGCAAGACATGTATTTAAAC
  ATAAACTAAAAGGAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_015946
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015946.4, NP_057030.3
• RefSeq Size: 2941 bp
• RefSeq ORF: 1158 bp
• Locus ID: 53918
• UniProt ID: Q9BRX2
• Cytogenetics: 5q11.2
• Domains: eRF1_1, eRF1_2, eRF1_3
• Protein Families: Druggable Genome
• Gene Summary: This gene encodes a protein which contains a conserved nuclear localization signal. The encoded protein may have a role in spermatogenesis, cell cycle control, and in meiotic cell division. [provided by RefSeq, Jul 2008]