USP30 (NM_032663) Human Untagged Clone

CAT#: SC320718

USP30 (untagged)-Human ubiquitin specific peptidase 30 (USP30)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: USP30
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_032663 edited
  CGGCCGCGAATTCGCACGAGGCCGTCGTATCCCTCGGTCCGGCGGCGGCGGCGGCGGTAG
  CGGAGGAGACGGTTTCAGGCCTCCGGTGCGGCTGCAATGCTGAGCTCCCGGGCCGAGGCG
  GCGATGACCGCGGCCGACAGGGCCATCCAGCGCTTCCTGCGGACCGGGGCGGCCGTCAGA
  TATAAAGTCATGAAGAACTGGGGAGTTATAGGTGGAATTGCTGCTGCTCTTGCAGCAGGA
  ATATATGTTATTTGGGGTCCCATTACAGAAAGAAAGAAGCGTAGAAAAGGGCTTGTGCCT
  GGCCTTGTTAATTTAGGGAACACCTGCTTCATGAACTCCCTGCTACAAGGCCTGTCTGCC
  TGTCCTGCTTTCATCAGGTGGCTGGAAGAGTTCACCTCCCAGTACTCCAGGGATCAGAAG
  GAGCCCCCCTCACACCAGTATTTATCCTTAACACTCTTGCACCTTCTGAAAGCCTTGTCC
  TGCCAAGAAGTTACTGATGATGAGGTCTTAGATGCAAGCTGCTTGTTGGATGTCTTAAGA
  ATGTACAGATGGCAGATCTCATCATTTGAAGAACAGGATGCTCACGAATTATTCCATGTC
  ATTACCTCGTCATTGGAAGATGAGCGAGACCGCCAGCCTCGGGTCACACATTTGTTTGAT
  GTGCATTCCCTGGAGCAGCAGTCAGAAATAACTCCCAAACAAATTACCTGCCGCACAAGA
  GGGTCACCTCACCCCACATCCAATCACTGGAAGTCTCAACATCCTTTTCATGGAAGACTC
  ACTAGTAATATGGTCTGCAAACACTGTGAACACCAGAGTCCTGTTCGATTTGATACCTTT
  GATAGCCTTTCACTAAGTATTCCAGCCGCCACATGGGGTCACCCATTGACCCTGGACCAC
  TGCCTTCACCACTTCATCTCATCAGAATCAGTGCGGGATGTTGTGTGTGACAACTGTACA
  AAGATTGAAGCCAAGGGAACGTTGAACGGGGAAAAGGTGGAACACCAGAGGACCACTTTT
  GTTAAACAGTTAAAACTAGGGAAGCTCCCTCAGTGTCTCTGCATCCACCTACAGCGGCTG
  AGCTGGTCCAGCCACGGCACGCCTCTGAAGCGGCATGAGCACGTGCAGTTCAATGAGTTC
  CTGATGATGGACATTTACAAGTACCACCTCCTTGGACATAAACCTAGTCAACACAACCCT
  AAACTGAACAAGAACCCAGGGCCTACACTGGAGCTGCAGGATGGGCCGGGAGCCCCCACA
  CCAGTTCTGAATCAGCCAGGGGCCCCCAAAACACAGATTTTTATGAATGGCGCCTGCTCC
  CCATCTTTATTGCCAACGCTGTCAGCGCCGATGCCCTTCCCTCTCCCAGTTGTTCCCGAC
  TACAGCTCCTCCACATACCTCTTCCGGCTGATGGCAGTTGTCGTCCACCATGGAGACATG
  CACTCTGGACACTTTGTCACTTACCGACGGTCCCCACCTTCTGCCAGGAACCCTCTCTCA
  ACTAGCAATCAGTGGCTGTGGGTCTCCGATGACACTGTCCGCAAGGCCAGCCTGCAGGAG
  GTCCTGTCCTCCAGCGCCTACCTGCTGTTCTACGAGCGCGTCCTTTCCAGGATGCAGCAC
  CAGAGCCAGGAGTGCAAGTCTGAAGAATGACTGTGCCCTCCTGCAAGGCTAGAGCTGATG
  GCACTGTCTGCACTGTCCAGGA
  
• Restriction Sites: Please inquire
• ACCN: NM_032663
• Insert Size: 2100 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032663.2, NP_116052.1
• RefSeq Size: 2117 bp
• RefSeq ORF: 1527 bp
• Locus ID: 84749
• UniProt ID: Q70CQ3
• Cytogenetics: 12q24.11
• Domains: UCH
• Protein Families: Protease, Transmembrane
• Gene Summary: USP30, a member of the ubiquitin-specific protease family (see USP1, MIM 603478), is a novel mitochondrial deubiquitinating (DUB) enzyme (Nakamura and Hirose, 2008 [PubMed 18287522]).[supplied by OMIM, Dec 2008]
  Transcript Variant: This variant (1) encodes the longer isoform (1).