PSMG1 (NM_003720) Human Untagged Clone

CAT#: SC320235

PSMG1 (untagged)-Human proteasome (prosome, macropain) assembly chaperone 1 (PSMG1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PSMG1
• Synonyms: C21LRP; DSCR2; LRPC21; PAC-1; PAC1
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_003720.2 CGGTCCGGCGGCGCTGCAGTTGTGTCCAGCCGGTCACGGGGCGGCTATGGCGGCCACGTT
  CTTCGGAGAGGTGGTGAAGGCGCCGTGCCGAGCTGGGACTGAGGACGAAGAGGAGGAGGA
  GGAGGGGCGGAGGGAGACGCCCGAGGACAGGGAGGTGCGTCTGCAGCTGGCGCGGAAGAG
  GGAAGTGCGGCTCCTTCGAAGACAAACAAAAACATCTTTGGAAGTTTCTTTGCTAGAAAA
  ATATCCGTGCTCCAAGTTTATAATTGCTATAGGAAATAATGCAGTAGCATTTCTGTCATC
  ATTTGTTATGAATTCAGGAGTCTGGGAGGAAGTTGGTTGTGCTAAACTCTGGAATGAATG
  GTGTAGAACAACAGACACTACACATCTGTCCTCCACAGAGGCTTTTTGTGTGTTTTATCA
  TCTAAAATCCAATCCCTCGGTTTTTCTCTGTCAGTGCAGTTGCTATGTTGCAGAAGATCA
  ACAGTATCAGTGGCTGGAAAAGGTTTTTGGCTCTTGTCCAAGGAAGAACATGCAGATAAC
  TATTCTCACATGTCGACATGTTACCGATTATAAAACCTCAGAATCCACCGGCAGCCTTCC
  TTCTCCTTTCCTGAGAGCCCTAAAAACACAGAATTTCAAAGACTCGGCGTGTTGTCCATT
  GCTAGAACAACCGAATATAGTACACGACCTTCCTGCAGCAGTTCTAAGCTACTGTCAAGT
  ATGGAAAATCCCGGCAATTCTGTACTTGTGTTATACTGATGTGATGAAATTAGACCTAAT
  CACAGTGGAAGCTTTTAAGCCTATACTTTCTACCAGAAGCTTGAAGGGTTTGGTTAAGAA
  TATTCCCCAAAGCACTGAGATACTAAAGAAATTGATGACAACAAATGAGATTCAGAGTAA
  CATTTATACATGATCTTAAACATTGTTTTGTAGTGTATATTACTTGTCCATTCCTTTAAG
  GGGAGCAGCCTGCACTCTTTTGTAGATTACTTTTGGGGGATATATTTTGAGAATGATGAA
  ACGGAATAAAATTGTAAAAAACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_003720
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003720.2, NP_003711.1
• RefSeq Size: 1146 bp
• RefSeq ORF: 867 bp
• Locus ID: 8624
• UniProt ID: O95456
• Cytogenetics: 21q22.2
• Gene Summary: Chaperone protein which promotes assembly of the 20S proteasome as part of a heterodimer with PSMG2. The PSMG1-PSMG2 heterodimer binds to the PSMA5 and PSMA7 proteasome subunits, promotes assembly of the proteasome alpha subunits into the heteroheptameric alpha ring and prevents alpha ring dimerization.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a).