Carboxypeptidase B2 (CPB2) (NM_001872) Human Untagged Clone

CAT#: SC319723

CPB2 (untagged)-Human carboxypeptidase B2 (plasma) (CPB2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Carboxypeptidase B2
• Synonyms: CPU; PCPB; TAFI
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001872.3 GTTGTACAGAAAATTGCTGTTGGGATGAAGCTTTGCAGCCTTGCAGTCCTTGTACCCATT
  GTTCTCTTCTGTGAGCAGCATGTCTTCGCGTTTCAGAGTGGCCAAGTTCTAGCTGCTCTT
  CCTAGAACCTCTAGGCAAGTTCAAGTTCTACAGAATCTTACTACAACATATGAGATTGTT
  CTCTGGCAGCCGGTAACAGCTGACCTTATTGTGAAGAAAAAACAAGTCCATTTTTTTGTA
  AATGCATCTGATGTCGACAATGTGAAAGCCCATTTAAATGTGAGCGGAATTCCATGCAGT
  GTCTTGCTGGCAGACGTGGAAGATCTTATTCAACAGCAGATTTCCAACGACACAGTCAGC
  CCCCGAGCCTCCGCATCGTACTATGAACAGTATCACTCACTAAATGAAATCTATTCTTGG
  ATAGAATTTATAACTGAGAGGCATCCTGATATGCTTACAAAAATCCACATTGGATCCTCA
  TTTGAGAAGTACCCACTCTATGTTTTAAAGGTTTCTGGAAAAGAACAAGCAGCCAAAAAT
  GCCATATGGATTGACTGTGGAATCCATGCCAGAGAATGGATCTCTCCTGCTTTCTGCTTG
  TGGTTCATAGGCCATATAACTCAATTCTATGGGATAATAGGGCAATATACCAATCTCCTG
  AGGCTTGTGGATTTCTATGTTATGCCGGTGGTTAATGTGGATGGTTATGACTACTCATGG
  AAAAAGAATCGAATGTGGAGAAAGAACCGTTCTTTCTATGCGAACAATCATTGCATCGGA
  ACAGACCTGAATAGGAACTTTGCTTCCAAACACTGGTGTGAGGAAGGTGCATCCAGTTCC
  TCATGCTCGGAAACCTACTGTGGACTTTATCCTGAGTCAGAACCAGAAGTGAAGGCAGTG
  GCTAGTTTCTTGAGAAGAAATATCAACCAGATTAAAGCATACATCAGCATGCATTCATAC
  TCCCAGCATATAGTGTTTCCATATTCCTATACACGAAGTAAAAGCAAAGACCATGAGGAA
  CTGTCTCTAGTAGCCAGTGAAGCAGTTCGTGCTATTGAGAAAACTAGTAAAAATACCAGG
  TATACACATGGCCATGGCTCAGAAACCTTATACCTAGCTCCTGGAGGTGGGGACGATTGG
  ATCTATGATTTGGGCATCAAATATTCGTTTACAATTGAACTTCGAGATACGGGCACATAC
  GGATTCTTGCTGCCGGAGCGTTACATCAAACCCACCTGTAGAGAAGCTTTTGCCGCTGTC
  TCTAAAATAGCTTGGCATGTCATTAGGAATGTTTAATGCCCCTGATTTTATCATTCTGCT
  TCCGTATTTTAATTTACTGATTCCAGCAAGACCAAATCATTGTATCAGATTATTTTTAAG
  TTTTATCCGTAGTTTTGATAAAAGATTTTCCTATTCCTTGGTTCTGTCAGAGAACCTAAT
  AAGTGCTACTTTGCCATTAAGGCAGACTAGGGTTCATGTCTTTTTACCCTTTAAAAAAAA
  TTGTAAAAGTCTAGTTACCTACTTTTTCTTTGATTTTCGACGTTTGACTAGCCATCTCAA
  GCAACTTTCGACGTTTGACTAGCCATCTCAAGCAAGTTTGATCAATGATCATCTCACGCT
  GATCATTGGATCCTACTCAACAAAAGGAAGGGTGGTCAGAAGTACATTAAAGATTTCTGC
  TCCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_001872
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001872.3, NP_001863.2
• RefSeq Size: 1766 bp
• RefSeq ORF: 1272 bp
• Locus ID: 1361
• UniProt ID: Q96IY4
• Cytogenetics: 13q14.13
• Domains: Zn_carbOpept, Propep_M14
• Protein Families: Druggable Genome, Protease, Secreted Protein
• Protein Pathways: Complement and coagulation cascades
• Gene Summary: Carboxypeptidases are enzymes that hydrolyze C-terminal peptide bonds. The carboxypeptidase family includes metallo-, serine, and cysteine carboxypeptidases. According to their substrate specificity, these enzymes are referred to as carboxypeptidase A (cleaving aliphatic residues) or carboxypeptidase B (cleaving basic amino residues). The protein encoded by this gene is activated by trypsin and acts on carboxypeptidase B substrates. After thrombin activation, the mature protein downregulates fibrinolysis. Polymorphisms have been described for this gene and its promoter region. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.