NT5C3L (NT5C3B) (NM_052935) Human Untagged Clone

CAT#: SC319648

NT5C3B (untagged)-Human 5'-nucleotidase, cytosolic III-like (NT5C3L), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NT5C3L
• Synonyms: cN-IIIB; NT5C3L
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_052935.2 GGATGGCAGAGGAGGTGAGGAGGGAGGAAGCGTCCGGAGTGGGGCGGGCCCGGACTCCGA
  CCCCCGGGGCGCTTCGAGCCCCCCAGCTGGTCACCGAGGCACCGCCGCTTCACCCAGGCC
  AGTAGCCGCCCCCCTCGCGCACCCCGGCCCCGCCTCACACGCGCGCCCGAGCGAGCCCCG
  GGCTCCCCTCGGGCCCAGCGTGGCGCAGGGGTCAGTGGTTCTCTCGGGTCTCGGGACAGG
  TGAGCACCCTGATGAAGGCCACGGTCCTGATGCGGCAGCCTGGGCGGGTGCAGGAGATCG
  TGGGCGCCCTCCGCAAGGGCGGCGGAGACCGGTTACAGGTGATTTCTGATTTTGACATGA
  CCTTGAGCAGGTTTGCATATAATGGAAAGCGATGCCCTTCTTCTTACAATATTCTGGATA
  ATAGCAAGATCATCAGTGAGGAGTGTCGGAAAGAGCTCACAGCGCTCCTTCACCACTATT
  ACCCAATTGAGATCGACCCACACCGGACCGTCAAGGAGAAGCTACCTCATATGGTGGAAT
  GGTGGACCAAAGCGCACAATCTCCTATGTCAGCAGAAGATTCAGAAGTTTCAGATAGCCC
  AGGTGGTTAGAGAGTCCAATGCAATGCTCAGGGAGGGATATAAGACCTTCTTCAACACAC
  TCTACCATAACAACATTCCCCTTTTCATCTTTTCTGCGGGCATTGGTGATATCCTGGAAG
  AAATTATCCGACAGATGAAAGTGTTCCACCCCAACATCCACATCGTGTCTAACTACATGG
  ATTTTAATGAAGATGGTTTTCTCCAGGGATTTAAGGGCCAGCTGATACACACATACAACA
  AGAACAGCTCTGTGTGTGAGAACTGTGGTTACTTCCAGCAACTTGAGGGCAAAACCAATG
  TCATCCTGCTGGGAGACTCTATCGGGGACCTCACCATGGCCGATGGGGTTCCTGGTGTGC
  AGAACATTCTCAAAATTGGCTTCCTGAATGACAAGGTGGAGGAGCGGCGGGAGCGCTACA
  TGGACTCCTATGACATCGTGCTGGAGAAGGACGAGACTCTGGATGTGGTCAACGGGCTAC
  TGCAGCACATCCTGTGCCAGGGGGTCCAGCTGGAGATGCAAGGCCCCTGAAGGCGCAGGC
  TCCAGCCCGGCCTGCAGGCCGTGGTGAGGAGGGGCGCCTCCCCAGAGTCTGCTCCCCCGT
  GAACACAGAGCAGAGGCCAGGGTGGCCAGCAGTGGCTGGGTCCTTCCGCGCCCCTCCGTC
  CTCCTTTCCCTGAGCACCTTCATCACCAGAGGCTTGAAGGAACCCCGCCATGTGGCAGGG
  CACAGGCACTGTTCCTGGTGAACCTTGGACCACAGCATGTCAGTGCTCTAGGGATTGTCT
  ACTCCAGGGATTTTCTTCAAAATTTTTAAACATGGGAAGTTCAAACAAATATAATGTGTG
  AAACAGAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_052935
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.

Reference Data

• RefSeq: NM_052935.2, NP_443167.2
• RefSeq Size: 1467 bp
• RefSeq ORF: 879 bp
• Locus ID: 115024
• UniProt ID: Q969T7
• Cytogenetics: 17q21.2
• Gene Summary: Specifically hydrolyzes 7-methylguanosine monophosphate (m(7)GMP) to 7-methylguanosine and inorganic phosphate (PubMed:23223233, PubMed:24603684). The specific activity for m(7)GMP may protect cells against undesired salvage of m(7)GMP and its incorporation into nucleic acids (PubMed:23223233). Also has weak activity for CMP (PubMed:23223233, PubMed:24603684). UMP and purine nucleotides are poor substrates (PubMed:23223233).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the shortest transcript but encodes the functional protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.