RECQ4 (RECQL4) (NM_004260) Human Untagged Clone

CAT#: SC318551

RECQL4 (untagged)-Human RecQ protein-like 4 (RECQL4)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RECQ4
• Synonyms: RECQ4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC318551 representing NM_004260.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGAGCGGCTGCGGGACGTGCGGGAGCGGCTGCAGGCGTGGGAGCGCGCGTTCCGACGGCAGCGCGGG
  CGGCGACCGAGCCAGGACGACGTGGAGGCGGCGCCGGAGGAGACCCGCGCGCTCTACCGGGAATACCGC
  ACTCTGAAGCGTACCACGGGCCAGGCCGGCGGCGGGCTCCGCAGCTCCGAGTCGCTCCCCGCGGCGGCC
  GAAGAGGCGCCAGAGCCCCGCTGCTGGGGGCCCCATCTGAATCGGGCTGCGACCAAGAGTCCACAGCCT
  ACGCCAGGGCGGAGCCGCCAGGGCTCGGTGCCGGACTACGGGCAGCGGCTCAAGGCCAATCTGAAAGGC
  ACCCTGCAGGCCGGACCAGCCCTGGGCCGCAGACCGTGGCCTCTAGGAAGAGCCTCATCTAAGGCATCC
  ACCCCAAAGCCCCCAGGTACAGGGCCTGTCCCCTCCTTTGCAGAAAAAGTCAGTGATGAGCCTCCACAG
  CTCCCTGAGCCCCAGCCAAGGCCAGGCCGGCTCCAGCATCTGCAGGCATCCCTGAGCCAGCGGCTGGGC
  TCCCTAGATCCTGGCTGGTTACAGCGATGTCACAGTGAGGTCCCAGATTTTCTGGGGGCCCCCAAAGCC
  TGCAGGCCTGATCTAGGCTCAGAGGAATCACAACTTCTGATCCCTGGTGAGTCGGCTGTCCTTGGTCCT
  GGTGCTGGCTCCCAGGGCCCAGAGGCTTCAGCCTTCCAAGAAGTCAGCATCCGTGTGGGGAGCCCCCAG
  CCCAGCAGCAGTGGAGGCGAGAAGCGGAGATGGAACGAGGAGCCCTGGGAGAGCCCCGCACAGGTCCAG
  CAGGAGAGCAGCCAAGCTGGACCCCCATCGGAGGGGGCTGGGGCTGTAGCAGTTGAGGAAGACCCTCCA
  GGGGAACCTGTACAGGCACAGCCACCTCAGCCCTGCAGCAGCCCATCGAACCCCAGGTACCACGGACTC
  AGCCCCTCCAGTCAAGCTAGGGCTGGGAAGGCTGAGGGCACAGCCCCCCTGCACATCTTCCCTCGGCTG
  GCCCGCCATGACAGGGGCAATTACGTACGGCTCAACATGAAGCAGAAACACTACGTGCGGGGCCGGGCA
  CTCCGTAGCAGGCTCCTCCGCAAGCAGGCATGGAAGCAGAAGTGGCGGAAGAAAGGGGAGTGTTTTGGG
  GGTGGTGGTGCCACAGTCACAACCAAGGAGTCTTGTTTCCTGAACGAGCAGTTCGATCACTGGGCAGCC
  CAGTGTCCCCGGCCAGCAAGTGAGGAAGACACAGATGCTGTTGGGCCTGAGCCACTGGTTCCTTCACCA
  CAACCTGTACCTGAGGTGCCCAGCCTGGACCCCACCGTGCTGCCACTCTACTCCCTGGGGCCCTCAGGG
  CAGTTGGCAGAGACGCCGGCTGAGGTGTTCCAGGCCCTGGAGCAGCTGGGGCACCAAGCCTTTCGCCCT
  GGGCAGGAGCGTGCAGTCATGCGGATCCTGTCTGGCATCTCCACGCTGCTGGTGCTGCCTACAGGTGCC
  GGCAAGTCCCTGTGCTACCAGCTCCCAGCGCTGCTCTACAGCCGGCGCAGCCCCTGCCTCACGTTGGTC
  GTCTCTCCCCTGCTGTCACTCATGGATGACCAGGTGTCTGGCCTGCCACCGTGTCTCAAGGCGGCCTGC
  ATACACTCGGGCATGACCAGGAAGCAACGGGAATCTGTCCTGCAGAAGATTCGGGCAGCCCAGGTACAC
  GTGCTGATGCTGACACCTGAGGCACTGGTGGGGGCGGGAGGCCTCCCTCCAGCCGCACAGCTGCCTCCA
  GTTGCTTTTGCCTGCATTGATGAGGCCCACTGCCTCTCCCAGTGGTCCCACAACTTCCGGCCCTGCTAC
  CTGCGCGTCTGCAAGGTGCTTCGGGAGCGCATGGGCGTGCACTGCTTCCTGGGCCTCACAGCCACAGCC
  ACACGCCGCACTGCCAGTGACGTGGCACAGCACCTGGCTGTGGCTGAAGAGCCTGACCTCCACGGGCCA
  GCCCCAGTTCCCACCAACCTGCACCTTTCCGTGTCCATGGACAGGGACACAGACCAGGCACTGTTGACG
  CTGCTGCAAGGCAAACGTTTTCAAAACCTCGATTCCATTATCATTTACTGCAACCGGCGCGAGGACACA
  GAGCGGATCGCTGCGCTCCTCCGAACCTGCCTGCACGCAGCCTGGGTCCCAGGGTCTGGAGGTCGTGCC
  CCCAAAACCACAGCCGAGGCCTACCACGCGGGCATGTGCAGCCGGGAACGGCGGCGGGTACAGCGAGCC
  TTCATGCAGGGCCAGTTGCGGGTGGTGGTGGCCACGGTGGCCTTTGGGATGGGGCTGGACCGGCCAGAT
  GTGCGGGCTGTGCTGCATCTGGGGCTGCCCCCAAGCTTCGAGAGCTACGTGCAGGCCGTGGGCCGGGCC
  GGGCGTGACGGGCAGCCTGCCCACTGCCACCTCTTCCTGCAGCCCCAGGGCGAAGACCTGCGAGAGCTG
  CGCAGACATGTGCACGCCGACAGCACGGACTTCCTGGCTGTGAAGAGGCTGGTACAGCGCGTGTTCCCA
  GCCTGCACCTGCACCTGCACCAGGCCGCCCTCGGAGCAGGAAGGGGCCGTGGGTGGGGAGAGGCCTGTG
  CCCAAGTACCCCCCTCAAGAGGCTGAGCAGCTTAGCCACCAAGCAGCCCCAGGACCCAGAAGGGTCTGC
  ATGGGCCATGAGCGGGCACTCCCAATACAGCTTACCGTACAGGCTTTGGACATGCCGGAGGAGGCCATC
  GAGACTTTGCTGTGCTACCTGGAGCTGCACCCACACCACTGGCTGGAGCTGCTGGCGACCACCTATACC
  CATTGCCGTCTGAACTGCCCTGGGGGCCCTGCCCAGCTCCAGGCCCTGGCCCACAGGTGTCCCCCTTTG
  GCTGTGTGCTTGGCCCAGCAGCTGCCTGAGGACCCAGGGCAAGGCAGCAGCTCCGTGGAGTTTGACATG
  GTCAAGCTGGTGGACTCCATGGGCTGGGAGCTGGCCTCTGTGCGGCGGGCTCTCTGCCAGCTGCAGTGG
  GACCACGAGCCCAGGACAGGTGTGCGGCGTGGGACAGGGGTGCTTGTGGAGTTCAGTGAGCTGGCCTTC
  CACCTTCGCAGCCCGGGGGACCTGACCGCTGAGGAGAAGGACCAGATATGTGACTTCCTCTATGGCCGT
  GTGCAGGCCCGGGAGCGCCAGGCCCTGGCCCGTCTGCGCAGAACCTTCCAGGCCTTTCACAGCGTAGCC
  TTCCCCAGCTGCGGGCCCTGCCTGGAGCAGCAGGATGAGGAGCGCAGCACCAGGCTCAAGGACCTGCTC
  GGCCGCTACTTTGAGGAAGAGGAAGGGCAGGAGCCGGGAGGCATGGAGGACGCACAGGGCCCCGAGCCA
  GGGCAGGCCAGACTCCAGGATTGGGAGGACCAGGTCCGCTGCGACATCCGCCAGTTCCTGTCCCTGAGG
  CCAGAGGAGAAGTTCTCCAGCAGGGCTGTGGCCCGCATCTTCCACGGCATCGGAAGCCCCTGCTACCCG
  GCCCAGGTGTACGGGCAGGACCGACGCTTCTGGAGAAAATACCTGCACCTGAGCTTCCATGCCCTGGTG
  GGCCTGGCCACGGAAGAGCTCCTGCAGGTGGCCCGCTGA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_004260
• Insert Size: 3627 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004260.3
• RefSeq Size: 3840 bp
• RefSeq ORF: 3627 bp
• Locus ID: 9401
• UniProt ID: O94761
• Cytogenetics: 8q24.3
• Protein Families: Druggable Genome, Stem cell - Pluripotency
• MW: 133.1 kDa
• Gene Summary: The protein encoded by this gene is a DNA helicase that belongs to the RecQ helicase family. DNA helicases unwind double-stranded DNA into single-stranded DNAs and may modulate chromosome segregation. This gene is predominantly expressed in thymus and testis. Mutations in this gene are associated with Rothmund-Thomson, RAPADILINO and Baller-Gerold syndromes. [provided by RefSeq, Jan 2010]