RPS6KC1 (NM_012424) Human Untagged Clone

CAT#: SC318476

RPS6KC1 (untagged)-Human ribosomal protein S6 kinase, 52kDa, polypeptide 1 (RPS6KC1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RPS6KC1
• Synonyms: humS6PKh1; RPK118; RSKL1; S6K-delta-1; S6PKh1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC318476 representing NM_012424.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGACCTCTTACCGGGAGCGGAGTGCCGACCTGGCCCGTTTCTACACTGTCACCGAGCCCCAGCGACAC
  CCGAGGGGCTACACAGTATATAAGGTCACCGCCCGGGTTGTTTCACGAAGAAATCCAGAGGATGTCCAG
  GAGATAATTGTATGGAAGAGATACAGTGATTTTAAGAAACTACACAAAGAACTATGGCAAATTCACAAA
  AACTTATTCCGACATTCAGAGTTGTTTCCTCCATTTGCTAAAGGAATAGTGTTTGGGCGATTTGATGAA
  ACTGTTATCGAAGAGAGAAGACAATGTGCTGAAGACCTGCTACAGTTCTCTGCCAATATTCCTGCTCTT
  TACAATAGTAAACAGCTTGAAGACTTTTTCAAGGGTGGAATAATTAATGATAGTTCTGAATTAATTGGT
  CCTGCTGAAGCTCACTCAGATTCCCTCATTGATACCTTTCCTGAGTGTAGTACGGAAGGCTTCTCCAGT
  GACAGTGATCTGGTATCTCTTACTGTTGATGTGGATTCTCTTGCTGAGTTAGATGATGGAATGGCTTCC
  AATCAAAATTCTCCCATTAGAACTTTTGGTCTCAATCTTTCTTCGGATTCTTCAGCACTAGGGGCTGTT
  GCTTCTGACAGTGAACAGAGCAAAACAGAAGAAGAACGGGAAAGTCGTAGCCTCTTTCCTGGCAGTTTA
  AAGCCGAAGCTTGGCAAGAGAGATTATTTGGAGAAAGCAGGAGAATTAATAAAGCTGGCTTTAAAAAAG
  GAAGAAGAAGACGACTATGAAGCTGCTTCTGATTTTTATAGGAAGGGAGTTGATTTACTCCTAGAAGGT
  GTTCAAGGAGAGTCAAGCCCTACCCGTCGAGAAGCTGTGAAGAGAAGAACAGCCGAGTACCTCATGCGG
  GCAGAAAGTATCTCTAGTCTTTATGGGAAACCTCAGCTTGATGATGTATCTCAGCCTCCAGGATCACTA
  AGTTCAAGGCCCCTTTGGAACCTAAGGAGCCCTGCCGAGGAGCTGAAGGCCTTCAGAGTCCTTGGGGTG
  ATTGACAAGGTTTTACTTGTAATGGACACAAGGACAGAACAGACTTTCATTTTAAAAGGTCTAAGGAAA
  AGCAGTGAATACAGCAGGAACAGAAAGACCATCATCCCCCGCTGTGTGCCCAACATGGTGTGTCTGCAT
  AAGTACATCATCTCTGAGGAGTCAGTATTTCTTGTGCTGCAGCATGCGGAAGGTGGCAAACTGTGGTCA
  TATATCAGTAAATTTCTAAACAGAAGTCCTGAAGAAAGCTTTGACATCAAGGAAGTGAAAAAACCTACA
  CTTGCAAAAGTTCACCTGCAGCAGCCAACTTCTAGTCCTCAGGACAGCAGTAGCTTTGAATCCAGAGGA
  AGTGATGGTGGAAGCATGCTTAAAGCTCTGCCTTTGAAGAGTAGTCTTACTCCAAGTTCTCAAGATGAC
  AGCAACCAGGAAGATGATGGCCAAGATAGCTCTCCAAAGTGGCCAGATTCTGGTTCAAGTTCAGAAGAA
  GAATGTACTACTAGTTATTTAACATTATGCAATGAATATGGGCAAGAAAAGATTGAACCAGGGTCTTTG
  AATGAGGAGCCCTTCATGAAGACTGAAGGGAATGGTGTTGATACAAAAGCTATTAAAAGCTTCCCAGCA
  CACCTTGCTGCTGACAGTGACAGCCCCAGCACACAGCTGAGAGCTCACGAGCTGAAGTTCTTCCCCAAC
  GATGACCCAGAAGCAGTTAGTTCTCCAAGAACATCAGATTCCCTCAGTAGATCAAAAAATAGCCCCATG
  GAATTCTTTAGGATAGACAGTAAGGATAGCGCAAGTGAACTCCTGGGACTTGACTTTGGAGAAAAATTG
  TATAGTCTAAAATCAGAACCTTTGAAACCATTCTTTACTCTTCCAGATGGAGACAGTGCTTCTAGGAGT
  TTTAATACTAGTGAAAGCAAGGTAGAGTTTAAAGCTCAGGACACCATTAGCAGGGGCTCAGATGACTCA
  GTGCCAGTTATTTCATTTAAAGATGCTGCTTTTGATGATGTCAGTGGTACTGATGAAGGAAGACCTGAT
  CTTCTTGTAAATTTACCTGGTGAATTGGAGTCAACAAGAGAAGCTGCAGCAATGGGACCTACTAAGTTT
  ACACAAACTAATATAGGGATAATAGAAAATAAACTCTTGGAAGCCCCTGATGTTTTATGCCTCAGGCTT
  AGTACTGAACAATGCCAAGCACATGAGGAGAAAGGCATAGAGGAACTGAGTGATCCCTCTGGGCCCAAA
  TCCTATAGTATAACAGAGAAACACTATGCACAGGAGGATCCCAGGATGTTATTTGTAGCAGCTGTTGAT
  CATAGTAGTTCAGGAGATATGTCTTTGTTACCCAGCTCAGATCCTAAGTTTCAAGGACTTGGAGTGGTT
  GAGTCAGCAGTAACTGCAAACAACACAGAAGAAAGCTTATTCCGTATTTGTAGTCCACTCTCAGGTGCT
  AATGAATATATTGCAAGCACAGACACTTTAAAAACAGAAGAAGTATTGCTGTTTACAGATCAGACTGAT
  GATTTGGCTAAAGAGGAACCAACTTCTTTATTCCAGAGAGACTCTGAGACTAAGGGTGAAAGTGGTTTA
  GTGCTAGAAGGAGACAAGGAAATACATCAGATTTTTGAGGACCTTGATAAAAAATTAGCACTAGCCTCC
  AGGTTTTACATCCCAGAGGGCTGCATTCAAAGATGGGCAGCTGAAATGGTGGTAGCCCTTGATGCTTTA
  CATAGAGAGGGAATTGTGTGCCGCGATTTGAACCCAAACAACATCTTATTGAATGATAGAGGACACATT
  CAGCTAACGTATTTTAGCAGGTGGAGTGAGGTTGAAGATTCCTGTGACAGCGATGCCATAGAGAGAATG
  TACTGTGCCCCAGAGGTTGGAGCAATCACTGAAGAAACTGAAGCCTGTGATTGGTGGAGTTTGGGTGCT
  GTCCTCTTTGAACTTCTCACTGGCAAGACTCTGGTTGAATGCCATCCAGCAGGAATAAATACTCACACT
  ACTTTGAACATGCCAGAATGTGTCTCTGAAGAGGCTCGCTCACTCATTCAACAGCTCTTGCAGTTCAAT
  CCTCTGGAACGACTTGGTGCTGGAGTTGCTGGTGTTGAAGATATCAAATCTCATCCATTTTTTACCCCT
  GTGGATTGGGCAGAACTGATGAGATGA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_012424
• Insert Size: 3201 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012424.2
• RefSeq Size: 4219 bp
• RefSeq ORF: 3201 bp
• Locus ID: 26750
• UniProt ID: Q96S38
• Cytogenetics: 1q32.3
• Domains: pkinase, PX, S_TKc, MIT
• Protein Families: Druggable Genome, Protein Kinase
• MW: 118.7 kDa
• Gene Summary: Sphingosine kinase catalyzes the formation of sphingosine 1 phosphate, a lipid cellular messenger. The protein encoded by this gene can bind to sphingosine kinase and to phosphatidylinositol 3-phosphate, suggesting a role in sphingosine 1 phophate signaling. The encoded protein can also bind to peroxiredoxin-3 and may help transport it to mitochondria. [provided by RefSeq, Mar 2017]
  Transcript Variant: This variant (1) encodes the longest isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.