UBA6 (NM_018227) Human Untagged Clone

CAT#: SC318471

UBA6 (untagged)-Human ubiquitin-like modifier activating enzyme 6 (UBA6)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: UBA6
• Synonyms: E1-L2; MOP-4; UBE1L2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC318471 representing NM_018227.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGAAGGATCCGAGCCTGTGGCCGCCCATCAGGGGGAAGAGGCGTCCTGTTCTTCCTGGGGGACTGGC
  AGCACAAATAAAAATTTGCCCATTATGTCAACAGCATCTGTGGAAATCGATGATGCATTGTATAGTCGA
  CAGAGGTACGTTCTTGGAGACACAGCAATGCAGAAGATGGCCAAGTCCCATGTTTTCTTAAGTGGGATG
  GGTGGTCTTGGTTTGGAAATTGCAAAGAATCTTGTTCTTGCAGGGATTAAGGCAGTTACAATTCATGAT
  ACAGAAAAATGCCAAGCATGGGATCTAGGAACCAACTTCTTTCTCAGTGAAGATGATGTTGTTAATAAG
  AGAAACAGGGCTGAAGCTGTACTTAAACATATTGCAGAACTAAATCCATACGTTCATGTCACATCATCT
  TCTGTTCCTTTCAATGAGACCACAGATCTCTCCTTTTTAGATAAATACCAGTGTGTAGTATTGACTGAG
  ATGAAACTTCCATTGCAGAAGAAGATCAATGACTTTTGCCGTTCTCAGTGCCCTCCAATTAAGTTTATC
  AGTGCAGATGTACATGGAATTTGGTCAAGGTTATTTTGTGATTTCGGTGATGAATTTGAAGTTTTAGAT
  ACAACAGGAGAAGAACCAAAAGAAATTTTCATTTCAAACATAACGCAAGCAAATCCTGGCATTGTTACT
  TGCCTTGAAAATCATCCTCACAAACTGGAGACAGGACAATTCCTAACATTTCGAGAAATTAATGGAATG
  ACAGGTTTAAATGGATCTATACAACAAATAACGGTGATATCGCCATTTTCTTTTAGTATTGGTGACACC
  ACAGAACTGGAACCATATTTACATGGAGGCATAGCTGTCCAAGTTAAGACTCCTAAAACAGTTTTTTTT
  GAATCACTGGAGAGGCAGTTAAAACATCCAAAGTGCCTTATTGTGGATTTTAGCAACCCTGAGGCACCT
  TTAGAGATTCACACAGCTATGCTTGCCTTGGACCAGTTTCAGGAGAAATACAGTCGCAAGCCAAATGTT
  GGATGCCAACAAGATTCAGAAGAACTGTTGAAACTAGCAACATCTATAAGTGAAACCTTGGAAGAGAAG
  CCTGATGTAAATGCTGACATTGTGCATTGGCTCTCTTGGACTGCCCAAGGCTTTTTATCTCCACTTGCT
  GCAGCAGTAGGAGGTGTTGCCAGCCAAGAAGTATTGAAAGCTGTAACAGGAAAATTTTCTCCTTTGTGC
  CAGTGGTTATATCTTGAAGCAGCAGATATTGTTGAATCACTAGGCAAACCTGAATGTGAAGAATTTCTC
  CCACGAGGAGATAGATATGATGCCTTAAGAGCTTGCATTGGAGACACTTTGTGTCAGAAACTGCAAAAT
  TTAAACATCTTCTTAGTAGGGTGTGGAGCCATAGGCTGTGAAATGTTGAAAAATTTTGCTTTACTTGGT
  GTTGGCACAAGCAAAGAGAAAGGAATGATTACAGTTACAGATCCTGACTTGATAGAGAAATCCAACTTA
  AATAGACAGTTCCTATTTCGTCCTCATCACATACAGAAACCTAAAAGCTACACTGCTGCTGATGCTACT
  CTGAAAATAAATTCTCAAATAAAGATAGATGCACACCTGAACAAAGTATGTCCAACCACTGAGACCATT
  TACAATGATGAGTTCTATACTAAACAAGATGTAATTATTACAGCATTAGATAATGTGGAAGCCAGGAGA
  TACGTAGACAGTCGTTGCTTAGCAAATCTAAGGCCTCTTTTAGATTCTGGAACAATGGGCACTAAGGGA
  CACACTGAAGTTATTGTACCGCATTTGACTGAGTCTTACAATAGTCATCGGGATCCCCCAGAAGAGGAA
  ATACCATTTTGTACTCTAAAATCCTTTCCAGCTGCTATTGAACATACCATACAGTGGGCAAGAGATAAG
  TTTGAAAGTTCCTTTTCCCACAAACCTTCATTGTTTAACAAATTTTGGCAAACCTATTCATCTGCAGAA
  GAAGTCTTACAGAAGATACAGAGTGGACACAGTTTAGAAGGCTGTTTTCAAGTTATAAAGTTACTTAGC
  AGAAGACCTAGAAATTGGTCCCAGTGTGTAGAATTAGCAAGATTAAAGTTTGAAAAATATTTTAACCAT
  AAGGCTCTTCAGCTTCTTCACTGTTTCCCTCTGGACATACGATTAAAAGATGGCAGTTTATTTTGGCAG
  TCACCAAAGAGGCCACCCTCTCCAATAAAATTTGATTTAAATGAGCCTTTGCACCTCAGTTTCCTTCAG
  AATGCTGCAAAACTATATGCTACAGTATATTGTATTCCATTTGCAGAAGAGGACTTATCAGCAGATGCC
  CTCTTGAATATTCTTTCAGAAGTAAAGATTCAGGAATTCAAGCCTTCCAATAAGGTTGTTCAAACAGAT
  GAAACTGCAAGGAAACCAGACCATGTTCCTATTAGCAGTGAAGATGAGAGGAATGCAATTTTCCAACTA
  GAAAAGGCTATTTTATCTAATGAAGCCACCAAAAGTGACCTTCAGATGGCAGTGCTTTCATTTGAAAAA
  GATGATGATCATAATGGACACATAGATTTCATCACAGCTGCATCAAATCTTCGTGCCAAAATGTACAGC
  ATTGAACCAGCTGACCGTTTCAAAACAAAGCGCATAGCTGGTAAAATTATACCTGCTATAGCAACAACC
  ACTGCTACAGTTTCTGGCTTGGTTGCCTTGGAGATGATCAAAGTAACTGGTGGCTATCCATTTGAAGCT
  TACAAAAATTGTTTTCTTAACTTAGCCATTCCAATTGTAGTATTTACAGAGACAACTGAAGTAAGGAAA
  ACTAAAATCAGAAATGGAATATCATTTACAATTTGGGATCGATGGACCGTACATGGAAAAGAAGATTTC
  ACCCTCTTGGATTTCATAAATGCAGTCAAAGAGAAGTATGGAATTGAGCCAACAATGGTGGTACAGGGA
  GTCAAAATGCTTTATGTTCCTGTAATGCCTGGTCATGCAAAAAGATTGAAGTTAACAATGCATAAACTT
  GTAAAACCTACTACTGAAAAGAAATATGTGGATCTTACTGTGTCATTTGCTCCAGACATTGATGGAGAT
  GAAGATTTGCCGGGACCTCCAGTAAGATACTACTTCAGTCATGACACTGATTAA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_018227
• Insert Size: 3159 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018227.5
• RefSeq Size: 6462 bp
• RefSeq ORF: 3159 bp
• Locus ID: 55236
• UniProt ID: A0AVT1
• Cytogenetics: 4q13.2
• Domains: UBACT, ThiF
• Protein Pathways: Ubiquitin mediated proteolysis
• MW: 118 kDa
• Gene Summary: Modification of proteins with ubiquitin (UBB; MIM 191339) or ubiquitin-like proteins controls many signaling networks and requires a ubiquitin-activating enzyme (E1), a ubiquitin conjugating enzyme (E2), and a ubiquitin protein ligase (E3). UBE1L2 is an E1 enzyme that initiates the activation and conjugation of ubiquitin-like proteins (Jin et al., 2007 [PubMed 17597759]).[supplied by OMIM, Mar 2008]