ERLIN1 (NM_006459) Human Untagged Clone

CAT#: SC317544

ERLIN1 (untagged)-Human ER lipid raft associated 1 (ERLIN1)


  "NM_006459" in other vectors (8)

Reconstitution Protocol

USD 503.00

3 Weeks*

Size
    • 10 ug

Product Images

Frequently bought together (4)
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Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
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Other products for "ERLIN1"

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol ERLIN1
Synonyms C10orf69; Erlin-1; KE04; KEO4; SPFH1; SPG62
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>SC317544 representing NM_006459.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGAATATGACTCAAGCCCGGGTTCTGGTGGCTGCAGTGGTGGGGTTGGTGGCTGTCCTGCTCTACGCC
TCCATCCACAAGATTGAGGAGGGCCATCTGGCTGTGTACTACAGGGGAGGAGCTTTACTAACTAGCCCC
AGTGGACCAGGCTATCATATCATGTTGCCTTTCATTACTACGTTCAGATCTGTGCAGACAACACTACAA
ACTGATGAAGTTAAAAATGTGCCTTGTGGAACAAGTGGTGGGGTCATGATCTATATTGACCGAATAGAA
GTGGTTAATATGTTGGCTCCTTATGCAGTGTTTGATATCGTGAGGAACTATACTGCAGATTATGACAAG
ACCTTAATCTTCAATAAAATCCACCATGAGCTGAACCAGTTCTGCAGTGCCCACACACTTCAGGAAGTT
TACATTGAATTGTTTGATCAAATAGATGAAAACCTGAAGCAAGCTCTGCAGAAAGACTTAAACCTCATG
GCCCCAGGTCTCACTATACAGGCTGTGCGTGTTACAAAACCCAAAATCCCAGAAGCCATAAGAAGAAAT
TTTGAGTTAATGGAGGCTGAGAAGACAAAACTCCTTATAGCTGCACAGAAACAAAAGGTTGTGGAAAAA
GAAGCTGAGACAGAGAGGAAAAAGGCAGTTATAGAAGCAGAGAAGATTGCACAAGTGGCAAAAATTCGG
TTTCAGCAGAAAGTGATGGAAAAAGAAACTGAAAAGCGCATTTCTGAAATCGAAGATGCTGCATTCCTG
GCCCGAGAGAAAGCGAAAGCAGATGCTGAATATTATGCTGCACACAAATATGCCACCTCAAACAAGCAC
AAGTTGACCCCGGAATATCTGGAGCTCAAAAAGTACCAGGCCATTGCTTCTAACAGTAAGATCTATTTT
GGCAGCAACATCCCTAACATGTTCGTGGACTCCTCATGTGCTTTGAAATATTCAGATATTAGGACTGGA
AGAGAAAGCTCACTCCCCTCTAAGGAGGCTCTTGAACCCTCTGGAGAGAACGTCATCCAAAACAAAGAG
AGCACAGGTTGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAG
GTTTAAACGGCCGGC
Restriction Sites SgfI-MluI     
ACCN NM_006459
Insert Size 1047 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_006459.3
RefSeq Size 3450 bp
RefSeq ORF 1047 bp
Locus ID 10613
UniProt ID O75477
Cytogenetics 10q24.31
Domains Band_7
Protein Families Transmembrane
MW 39.2 kDa
Gene Summary The protein encoded by this gene is part of a protein complex that mediates degradation of inositol 1,4,5-trisphosphate receptors in the endoplasmic reticulum. The encoded protein also binds cholesterol and regulates the SREBP signaling pathway, which promotes cellular cholesterol homeostasis. Defects in this gene have been associated with spastic paraplegia 62. [provided by RefSeq, Dec 2016]
Transcript Variant: This variant (1) represents the longest transcript. Variants 1-6 all encode the same isoform (a).

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.