DTX2 (NM_001102595) Human Untagged Clone

CAT#: SC316953

DTX2 (untagged)-Human deltex homolog 2 (Drosophila) (DTX2), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DTX2
• Synonyms: RNF58
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC316953 representing NM_001102595.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGCCATGGCCCCAAGCCCTTCCCTGGTGCAGGTGTACACCAGCCCCGCGGCTGTGGCCGTGTGGGAA
  TGGCAGGACGGGCTGGGCACCTGGCACCCCTACAGTGCCACCGTCTGCAGCTTCATCGAGCAGCAGTTT
  GTCCAGCAGAAGGGCCAACGTTTTGGGCTTGGGAGCCTGGCCCACAGCATCCCCTTGGGCCAGGCAGAC
  CCCTCGCTGGCCCCTTACATTATTGACCTCCCCAGCTGGACCCAGTTCCGCCAGGACACCGGCACCATG
  CGGGCTGTGCGGAGACACCTGTTCCCCCAGCACTCAGCCCCTGGCCGAGGTGTCGTCTGGGAGTGGCTG
  AGCGACGATGGCTCCTGGACTGCCTATGAAGCCAGCGTCTGTGACTATCTGGAGCAGCAGGTGGCCAGG
  GGCAACCAGCTCGTGGACTTGGCCCCCCTGGGGTACAACTACACTGTCAACTACACCACCCACACGCAG
  ACCAACAAGACTTCCAGCTTCTGCCGCAGCGTGCGGCGCCAAGCAGGGCCGCCTTACCCGGTGACCACC
  ATCATCGCTCCGCCGGGCCACACAGGCGTCGCCTGCTCTTGCCACCAGTGCCTCAGTGGCAGCAGAACT
  GGCCCCGTGTCAGGCCGCTACCGCCACTCCATGACCAACCTCCCTGCATACCCCGTCCCCCAGCACCCC
  CCACACAGGACCGCTTCTGTGTTTGGGACCCACCAGGCCTTTGCACCGTACAACAAACCCTCACTCTCC
  GGGGCCCGGTCTGCGCCCAGGCTGAACACCACCAACGCCTGGGGCGCAGCTCCTCCTTCCCTGGGGAGC
  CAGCCCCTCTACCGCTCCAGCCTCTCCCACCTGGGACCGCAGCACCTGCCCCCAGGATCCTCCACCTCC
  GGTGCAGTCAGTGCCTCCCTCCCCAGCGGTCCCTCAAGCAGCCCAGGGAGCGTCCCTGCCACTGTGCCC
  ATGCAGATGCCAAAGCCCAGCAGAGTCCAGCAGGCGCTCGCAGGCATGACGAGTGTTCTGATGTCAGCC
  ATTGGACTCCCTGTGTGTCTTAGCCGCGCACCCCAGCCCACCAGCCCTCCCGCCTCCCGTCTGGCTTCC
  AAAAGTCACGGCTCAGTTAAGAGATTGAGGAAAATGTCCGTGAAAGGAGCGACCCCGAAGCCAGAGCCA
  GAGCCAGAGCAGGTCATAAAAAACTACACGGAAGAGCTGAAAGTGCCCCCAGATGAGGACTGCATCATC
  TGCATGGAGAAGCTGTCCACAGCGTCTGGATACAGCGATGTGACTGACAGCAAGGCAATCGGGTCCCTA
  GCTGTGGGCCACCTCACCAAGTGCAGCCATGCCTTCCACCTGCTGTGCCTCCTGGCCATGTACTGCAAC
  GGCAATAAGGATGGAAGTCTGCAGTGTCCCTCCTGCAAAACCATCTATGGAGAGAAGACGGGGACCCAG
  CCCCAGGGAAAGATGGAGGTATTACGGTTCCAGATGTCGCTCCCCGGCCACGAGGACTGCGGGACCATC
  CTCATAGTTTACAGCATTCCCCATGGTATCCAGGGCCCTGAGCACCCCAATCCCGGAAAGCCGTTCACT
  GCCAGAGGGTTTCCCCGCCAGTGCTACCTTCCAGACAACGCCCAGGGCCGCAAGGTCCTAGAGCTCCTG
  AAGGTGGCCTGGAAGAGGCGGCTCATCTTCACAGTGGGCACGTCCAGCACCACGGGTGAGACGGACACC
  GTGGTATGGAACGAGATCCACCACAAGACAGAGATGGACCGCAACATTACGGGCCACGGCTATCCCGAC
  CCCAACTACCTGCAGAACGTGCTGGCTGAGCTGGCTGCCCAGGGGGTGACCGAGGACTGCCTGGAGCAG
  CAGTGA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001102595
• Insert Size: 1869 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001102595.1
• RefSeq Size: 2550 bp
• RefSeq ORF: 1869 bp
• Locus ID: 113878
• UniProt ID: Q86UW9
• Cytogenetics: 7q11.23
• Protein Families: Druggable Genome
• Protein Pathways: Notch signaling pathway
• MW: 67.2 kDa
• Gene Summary: DTX2 functions as an E3 ubiquitin ligase (Takeyama et al., 2003 [PubMed 12670957]).[supplied by OMIM, Nov 2009]
  Transcript Variant: This variant (3) differs in the 5' UTR, compared to variant 1, and encodes isoform A. Variants 1-3 encode the same isoform (A).