SCC112 (PDS5A) (NM_001100399) Human Untagged Clone

CAT#: SC316869

PDS5A (untagged)-Human PDS5, regulator of cohesion maintenance, homolog A (S. cerevisiae) (PDS5A), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PDS5A
• Synonyms: PIG54; SCC-112; SCC112
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001100399, the custom clone sequence may differ by one or more nucleotides
  ATGGACTTCACCGCGCAGCCCAAGCCTGCCACTGCCCTCTGTGGCGTCGTGAGTGCCGAC
  GGGAAGATCGCTTACCCTCCGGGGGTAAAAGAGATCACCGACAAGATCACCACGGACGAG
  ATGATCAAACGCCTGAAGATGGTAGTGAAAACCTTTATGGATATGGATCAGGACTCAGAA
  GATGAAAAACAGCAGTATCTCCCACTAGCCTTGCATCTTGCATCTGAATTCTTCCTCAGG
  AACCCCAATAAAGATGTGCGTCTCCTTGTAGCATGTTGTTTGGCTGATATCTTTCGTATC
  TATGCCCCAGAAGCTCCATATACTTCCCATGATAAACTTAAGGACATATTTTTGTTTATT
  ACCAGACAATTAAAAGGTTTGGAGGATACAAAGAGTCCACAGTTTAATAGATACTTTTAT
  TTATTAGAGAATTTAGCTTGGGTTAAATCATATAACATCTGCTTTGAATTGGAAGATTGC
  AATGAAATTTTTATTCAGCTTTTTAGAACTCTCTTCTCAGTGATCAACAATAGCCACAAT
  AAGAAGGTACAAATGCACATGCTAGATTTGATGAGTTCTATCATCATGGAAGGTGATGGA
  GTTACTCAAGAATTATTGGACTCCATTCTTATTAACCTCATTCCTGCACATAAGAACTTA
  AATAAACAGTCCTTTGACCTTGCAAAAGTGCTATTGAAAAGAACAGTCCAGACTATTGAG
  GCATGCATTGCTAATTTTTTCAATCAAGTCCTGGTGCTGGGAAGATCATCAGTAAGTGAT
  TTGTCAGAACATGTATTTGATCTGATTCAGGAACTTTTTGCTATAGATCCTCATTTATTA
  TTATCCGTCATGCCACAGCTTGAATTCAAACTAAAGAGCAATGATGGAGAAGAGCGATTA
  GCTGTTGTTCGACTTCTAGCTAAATTGTTTGGCTCCAAAGATTCTGATTTGGCAACACAG
  AATCGTCCTCTTTGGCAATGTTTTCTTGGACGATTTAATGATATTCATGTTCCTGTGAGA
  TTAGAAAGTGTGAAATTTGCCAGTCATTGTTTAATGAATCACCCAGATTTAGCGAAGGAT
  CTCACAGAATATTTAAAGGTTAGATCACATGATCCAGAAGAAGCTATTCGTCATGATGTC
  ATTGTTACTATAATAACAGCTGCCAAGAGGGACCTGGCCTTAGTAAATGATCAGCTGCTT
  GGCTTTGTAAGGGAAAGAACACTGGATAAACGGTGGCGAGTAAGAAAAGAAGCTATGATG
  GGTCTGGCTCAGCTTTATAAGAAATACTGTCTTCATGGTGAAGCAGGAAAGGAAGCTGCA
  GAGAAAGTCAGCTGGATAAAGGACAAACTTCTGCATATTTATTATCAGAACAGCATTGAC
  GACAAACTGTTGGTAGAGAAAATCTTTGCTCAGTATCTTGTCCCCCACAACCTGGAAACA
  GAAGAGAGAATGAAATGCTTATATTACTTATATGCTAGTTTGGATCCAAATGCTGTAAAA
  GCTCTCAACGAAATGTGGAAGTGTCAGAACATGCTTCGGAGCCATGTACGCGAACTATTG
  GATTTGCACAAGCAGCCTACATCAGAGGCTAACTGTTCTGCCATGTTTGGAAAACTGATG
  ACCATAGCAAAGAATTTGCCTGACCCCGGGAAAGCACAAGATTTTGTGAAGAAATTTAAC
  CAGGTTCTCGGCGATGATGAGAAACTTCGGTCTCAGTTGGAGTTATTAATTAGCCCAACC
  TGTTCTTGCAAACAAGCAGATATTTGTGTGAGAGAAATAGCCCGGAAACTTGCAAATCCT
  AAGCAACCAACAAATCCTTTTCTAGAGATGGTCAAATTTCTGTTGGAAAGAATCGCACCT
  GTGCACATTGATTCAGAAGCCATAAGTGCACTAGTGAAATTGATGAATAAGTCAATAGAG
  GGGACAGCAGATGATGAAGAGGAGGGTGTAAGTCCAGATACAGCTATCCGTTCAGGACTT
  GAACTTCTTAAGGTTCTGTCTTTTACACATCCTACCTCGTTCCACTCTGCAGAGACATAT
  GAGTCCTTGTTACAGTGCCTAAGAATGGAGGATGACAAGGTAGCAGAAGCTGCTATTCAA
  ATTTTTAGAAATACAGGTCACAAAATAGAAACAGACCTTCCCCAGATACGATCGACCTTA
  ATTCCCATTTTACATCAAAAAGCAAAGAGGGGTACTCCACACCAAGCAAAACAGGCTGTG
  CACTGTATACACGCCATATTCACAAATAAAGAAGTCCAGCTTGCACAGATTTTTGAGCCA
  CTCAGTAGGAGTCTGAATGCTGATGTGCCAGAACAACTTATAACTCCATTAGTTTCATTG
  GGCCACATTTCTATGTTAGCACCAGATCAGTTTGCTTCCCCAATGAAATCTGTAGTAGCA
  AATTTTATTGTGAAAGATCTGCTAATGAATGACAGGTCAACAGGTGAAAAGAATGGAAAA
  CTGTGGTCTCCAGATGAAGAGGTTTCCCCTGAAGTACTAGCAAAGGTACAGGCAATTAAA
  CTTCTGGTAAGGTGGCTGTTGGGTATGAAAAACAACCAGTCTAAATCTGCCAATTCAACC
  CTTCGGTTATTATCAGCGATGTTGGTTAGTGAGGGTGACCTGACAGAGCAAAAGAGGATC
  AGTAAATCTGATATGTCTCGCTTGCGATTAGCTGCTGGTAGTGCCATAATGAAGCTTGCT
  CAGGAACCTTGTTACCATGAAATTATTACCCCAGAACAGTTTCAGCTCTGTGCACTTGTT
  ATTAATGATGAGTGTTACCAAGTAAGGCAGATATTTGCTCAGAAGCTGCATAAGGCACTT
  GTGAAGTTACTGCTCCCATTGGAGTATATGGCGATCTTTGCCTTGTGTGCCAAAGATCCT
  GTGAAGGAGAGAAGAGCACACGCACGACAATGTTTACTGAAAAATATCAGTATACGCAGG
  GAATACATTAAGCAGAATCCTATGGCTACTGAGAAATTATTATCACTGTTGCCTGAATAT
  GTAGTTCCATACATGATTCACCTGCTAGCCCATGATCCAGATTTTACAAGATCACAAGAT
  GTTGATCAGCTTCGTGATATCAAAGAGTGCCTATGGTTCATGCTTGAAGTTTTAATGACA
  AAGAATGAAAACAATAGCCATGCCTTTATGAAGAAGATGGCAGAGAACATCAAGTTAACC
  AGAGATGCCCAGTCTCCAGATGAATCCAAGACAAATGAAAAACTGTATACAGTATGTGAT
  GTGGCTCTCTGTGTTATAAATAGTAAAAGTGCTTTGTGCAATGCAGATTCACCAAAGGAC
  CCAGTCCTCCCAATGAAATTTTTTACACAACCTGAAAAGGACTTCTGTAACGATAAGAGT
  TATATTTCAGAAGAGACAAGAGTACTTCTGTTAACAGGAAAGCCAAAGCCTGCTGGAGTA
  CTAGGTGCAGTAAATAAGCCTTTATCAGCAACGGGAAGGAAACCCTATGTTAGAAGCACT
  GGCACTGAGACTGGAAGCAATATTAATGTAAATTCAGAGCTGAACCCTTCAACCGGAAAT
  CGATCAAGGGAACAGAGTTCAGAGGCAGCAGAAACTGGAGTTAGTGAAAATGAAGAGAAC
  CCTGTGAGGATTATTTCAGTCACACCTGTAAAGAATATTGACCCAGTAAAGAATAAGGAA
  ATTAATTCTGATCAGGCTACCCAGGGCAACATCAGCAGTGACCGAGGAAAGAAAAGAACA
  GTAACAGCAGCTGGTGCAGAGAATATCCAACAAAAAACAGATGAGAAAGTAGATGAATCG
  GGACCTCCCGCCCCTTCCAAACCCAGGAGAGGACGTCGACCCAAGTCTGAATCTCAGGGC
  AATGCTACCAAAAATGATGATCTAAATAAACCTATTAACAAGGGAAGGAAGAGAGCTGCA
  GTGGGTCAGGAGAGCCCTGGGGGTTTGGAAGCAGGTAATGCCAAAGCACCCAAACTGCAA
  GATTTAGCCAAAAAGGCAGCACCAGCAGAAAGACAAATTGACTTACAAAGG
  
• Restriction Sites: Please inquire
• ACCN: NM_001100399
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001100399.1, NP_001093869.1
• RefSeq Size: 7190 bp
• RefSeq ORF: 4014 bp
• Locus ID: 23244
• UniProt ID: Q29RF7
• Cytogenetics: 4p14
• Gene Summary: The protein encoded by this gene binds to the cohesin complex and associates with chromatin through most of the cell cycle. The encoded protein may play a role in regulating sister chromatid cohesion during mitosis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2010]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).