TCTN1 (NM_001082537) Human Untagged Clone

CAT#: SC316010

TCTN1 (untagged)-Human tectonic family member 1 (TCTN1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TCTN1
• Synonyms: JBTS13; TECT1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC316010 representing NM_001082537.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGAGGCCGCGAGGTCTCCCGCCGCTCCTGGTGGTGCTCCTGGGCTGCTGGGCCTCCGTGAGCGCCCAG
  ACCGATGCCACCCCGGCGGTGACGACAGAGGGCCTCAACTCCACCGAGGCAGCCCTGGCCACCTTCGGA
  ACTTTCCCGTCGACCAGGCCCCCCGGGACTCCCAGGGCTCCAGGGCCCTCCTCCGGCCCCAGGCCTACC
  CCAGTCACGGACGTTGCTGTTCTCTGTGTCTGTGACTTATCCCCAGCACAGTGTGACATCAACTGCTGC
  TGTGATCCCGACTGCAGCTCCGTGGATTTCAGTGTCTTTTCTGCCTGCTCAGTTCCAGTTGTCACGGGC
  GACAGCCAGTTTTGTAGTCAAAAAGCAGTCATCTATTCATTGAATTTTACAGCAAACCCACCTCAAAGA
  GTATTTGAACTTGTTGACCAGATTAATCCATCTATTTTCTGCATTCATATTACAAACTATAAACCTGCA
  TTATCCTTTATTAATCCAGAAGTACCTGATGAAAACAATTTTGATACATTGATGAAAACATCTGATGGT
  TTTACATTGAATGCTGAATCATATGTTTCCTTCACAACCAAACTGGATATTCCTACTGCTGCTAAATAT
  GAGTATGGGGTTCCTCTGCAGACTTCAGATTCGTTTCTGAGATTTCCTTCGTCCCTGACATCATCTCTG
  TGCACTGATAATAACCCTGCAGCGTTTCTGGTGAACCAGGCTGTTAAGTGCACCAGAAAAATAAATTTA
  GAACAGTGTGAAGAAATTGAAGCCCTCAGCATGGCTTTTTACAGCAGCCCGGAAATTCTGAGGGTACCT
  GATTCAAGAAAAAAGGTCCCTATCACTGTTCAGTCCATCGTCATTCAGTCTCTAAATAAAACGCTCACC
  CGACGGGAGGACACTGATGTGCTGCAGCCGACTCTCGTCAACGCTGGACACTTTAGCCTTTGCGTGAAT
  GTTGTTCTTGAGGTAAAGTACAGCCTCACATACACAGATGCAGGTGAAGTCACCAAAGCTGATCTCTCA
  TTCGTTCTGGGGACAGTTAGCAGCGTAGTGGTCCCACTGCAGCAAAAGTTTGAAATTCATTTTCTTCAG
  GAAAATACCCAGCCAGTCCCTCTCAGTGGAAACCCTGGTTATGTCGTGGGGCTCCCATTAGCTGCTGGA
  TTCCAGCCTCATAAGGGGTCTGGGATTATTCAGACCACAAATAGATATGGACAGCTTACTATTCTTCAT
  AGCACAACTGAGCAAGACTGCTTAGCACTGGAGGGGGTCCGGACCCCAGTATTATTTGGTTACACTATG
  CAATCTGGCTGTAAACTAAGACTGACTGGAGCTCTCCCGTGTCAGCTCGTAGCACAGAAGGTGAAGAGC
  CTGCTGTGGGGCCAGGGCTTCCCAGATTACGTGGCCCCTTTTGGAAATTCCCAGGCCCAGGACATGCTG
  GACTGGGTGCCCATCCACTTCATCACCCAGTCATTCAACAGGAAGGATTCCTGCCAGCTCCCAGGGGCT
  TTGGTTATAGAAGTGAAGTGGACTAAATACGGATCCCTGCTGAATCCACAGGCCAAAATAGTCAATGTA
  ACTGCAAATCTAATTTCATCCTCCTTTCCTGAGGCCAACTCAGGAAATGAAAGGACGATTCTTATTTCC
  ACTGCGGTTACTTTTGTGGATGTGTCTGCACCTGCAGAGGCAGGCTTCAGAGCTCCACCAGCCATCAAT
  GCCAGGCTGCCCTTTAACTTCTTCTTCCCGTTTGTTTGA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001082537
• Insert Size: 1764 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001082537.2
• RefSeq Size: 2025 bp
• RefSeq ORF: 1764 bp
• Locus ID: 79600
• UniProt ID: Q2MV58
• Cytogenetics: 12q24.11
• Protein Families: Secreted Protein
• MW: 63.6 kDa
• Gene Summary: This gene encodes a member of a family of secreted and transmembrane proteins. The orthologous gene in mouse functions downstream of smoothened and rab23 to modulate hedgehog signal transduction. This protein is a component of the tectonic-like complex, which forms a barrier between the ciliary axoneme and the basal body. A mutation in this gene was found in a family with Joubert syndrome-13. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016]
  Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1, resulting in a shorter protein (isoform 2).