TMC3 (NM_001080532) Human Untagged Clone

CAT#: SC315868

TMC3 (untagged)-Human transmembrane channel-like 3 (TMC3)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TMC3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC315868 representing NM_001080532.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGAAAACCTCGAAGGCATCCCAGCGCTATAGAGGCATCCGGAGAAATGCCAGCCAGTGCTACCTCTAC
  CAGGAATCTCTGCTGCTCAGCAACTTGGATGACAGCTTTAGTGCTGATGAAACAGGGGACAGCAATGAT
  CCGGAACAAATCTTCCAGAATATACAGTTCCAGAAAGATCTCATGGCAAACATTCGCTGCAGACCCTGG
  ACTATGGGACAGAAGCTGAGGGCATTGAGACAAGCGAAGAACATTGTGCTGAAGTTTGAAGGGAGGCTG
  ACCAGGACCCGAGGCTACCAAGCAGCAGGTGCAGAGCTCTGGCGGAAATTTGCTCGTCTCGCCTGTAAC
  TTTGTGGTCATCTTCATTCCCTGGGAAATGAGGATAAAGAAAATCGAGAGTCATTTTGGATCTGGCGTT
  GCCTCCTATTTCATATTCTTGAGATGGTTATTTGGAATTAATATTGTGCTCACCATTATGACAGGTGCT
  TTTATTGTCATTCCAGAGCTGATTGCAGGCCAGCCCTTTGGAAGCACAGCCAGGAAGACCATCCCCAAG
  GAGCAGGTTTCGTCTGCCCAAGACCTGGACACCGTCTGGTCTCTGGGGGGCTACCTCCAGTACTCTGTC
  CTCTTCTACGGATATTACGGCAGGGAGAGGAAGATCGGGAGAGCTGGCTACCGGCTGCCCTTGGCGTAT
  TTCCTAGTGGGGATGGCAGTGTTTGCTTACAGCTTCATCATTCTTTTAAAAAAGATGGCTAAGAACTCC
  CGCACGAGTCTTGCCAGTGCTTCCAATGAAAACTATACCTTCTGCTGGCGGGTGTTCTGTGCCTGGGAT
  TACCTCATTGGAAACCCAGAGGCTGCAGAGAGCAAAACAGCTGCCATAGTGAACAGCATCAGGGAAGCT
  ATATTGGAAGAACAGGAGAAAAAGAAAAGCAAAAACCTGGCAGTGACCATCTGCCTGAGGATTATTGCC
  AACATCCTGGTGCTTCTCTCACTGGCTGGGAGCATTTATCTCATCTACTTTGTGGTGGACCGGTCCCAG
  AAGCTGGAGCAGTCGAAGAAGGAGCTGACACTTTGGGAAAAGAATGAGGTCAGTGTGGTGGTCTCCCTC
  GTCACCATGATAGCACCATCAGCCTTTGACCTCATTGCTGCCTTAGAGATGTACCACCCCAGGACCACG
  CTGCGCTTCCAGCTTGCAAGGGTCCTTGTGCTGTATTTGGGAAATCTCTACAGCCTGATCATTGCTCTC
  CTGGACAAAGTTAACAGCATGAGCATTGAGGAAATGGCTACCAAAAATAACACAAGTCATTGGATAGAT
  TCCACCACCTTCTTTGCAACCAGGACAGCCCCTGAAGAAGAGAAATGGTCCACATCTCGGCCTGGAATG
  GGGCTCAGGAGAAACAACACATGGGCCTTGGAAGAGACCAGCATTTCAGCTTATACCATGCCTCTTATA
  AAGGCCAACAAGACTAGCCTCCACACTCAGAGTCCACAAGACCAGTGCTGGGAGACATACGTTGGTCAG
  GAGATGCTGAAGCTCTCCATCATTGACATGCTCTTCACCGTGGCGAGCATTCTGCTCATAGACTTCTTC
  CGAGGACTTTTCGTGCGGTACTTAAGTGACTACTGGTGTTGGGATCTGGAGAGCAAGTTTCCTGAATAT
  GGAGAATTCAAAATAGCTGAGAATGTGCTACATTTAGTCTACAACCAAGGAATGATTTGGATGGGGGCC
  TTCTTCTCCCCATGTCTCCCAGCGTTCAACGTTCTCAAACTCATTGGGCTCATGTACCTGAGAAGCTGG
  GCTGTGCTGACCTGCAATGTGCCCCACCAGCAAGTATTTCGAGCCTCAAGATCCAACAACTTCTACTTG
  GCAATGCTCCTGTTTATGCTGTTTCTGTGCATGCTGCCAACCATTTTTGCTATTGTCCGATACAAGCCA
  TCTCTAAACTGTGGGCCATTCAGTGGACAAGAGAAAATTTATGACATTGTGTCAGAAACGATTGAGAAA
  GACTTTCCTGTGTGGTTTGGCTCCGTGGTTGGACACATCAGTAGCCCCGTGGTCATCCTGCCCGCAGTA
  CTCCTGCTTTTCATGCTCATCTATTATCTCCAGAGCATCGCACGGTCTCTAAAGCTCAGCAACCACCAG
  CTCAAAATGCAGATCCAAAACGCAAGATCAGAGGATAAGAAAAAGGTTGCCCAGATGGTAGAAGCCCGA
  ATCCAGACCCAGGAAGAAAGCACCAAGAAGCTTCCAAACGACAGTGATCTTACCAGCCAGCTGTCCTCA
  GCGCACTCGGGCACACCCCAAAACAACGGCAACGTGGCCCATTTTGACTCAGGGAGCAGCAAGAGTGGC
  AGGATAGAGACAGTCGCACAGTCCATGCCCCAGAGCCCGAGGCCAGGGGACAGGGCTCCTAGCTCACCT
  CTCCCTGGGGTCCCCAAATCCAGGCTAGAGCATGAAACTAACAGGTATCTGCACGGTCTGTGTGCAAGC
  ACCAGTGACCTTCACAGGAACAGATCGCGCACACCTATGACATTTACAACGCACATCGAAGATGTACAC
  TCAGAACCTCTTTTCCGAAAAGACTTTCAGCAAATCAACCCTCCTCACCGTGGACCACAGGCCTCGACT
  TTGCTGGCACAGGGTCCCAGGCCCCACGCCCCCAGATACTATGTCATTAATGAATGTGACTCTTACAAG
  AAAAAACATCTAAATGTCTGGCCAGAAAGACATTTCAAGATAGATGCTTCAGGTGACATTGTGGAGCTG
  TACCCCAGGAACGTGCGACAATATGCATCCCGGGTCCCCCGCCAGCCTCCCTCCCCACAGCTGAGTGAA
  GAAGAGGAGGAGACGCCAAGCAGAGATTGGATCAAACGGTCTCTTCCCCCACGCTCCCTGATAGACCTC
  CGTCGGGCTCCTCATTTTTATATTGGGGAGAGGTCCGAAAGTCAGACCCGGGATCCCGAGCACCAGGGG
  AGGGTGCACTACAAGTCGTGGAATGAAGATTTTGAGGGTCACCTTGAGAGGCCAGCCTATGTGCCCAGA
  AAGCCACGATCCCGGAATTTCCAATACCCACAGCCCCCTCTGAAGCCCAGAGGGAAGCCCAGGTTCGAG
  CCATCCCTCACGGAATCTGACTCCGTGTCGGCAGCATCCAGCAGTGACCAGCAGAACAGCAGCGCTGAC
  CAGTACCTGCAGGTCACCCACAGCCAGGGCAGGTTCCCGAGGTCCGTGGGCCAGCCCAGCAGGAGGAAG
  GCCAAGTCGGGGCAGGAGCTGACCGTGGATCTGGATGACTTGATTTGTTCAGATGTCTAG
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001080532
• Insert Size: 3303 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001080532.1
• RefSeq Size: 3303 bp
• RefSeq ORF: 3303 bp
• Locus ID: 342125
• UniProt ID: Q7Z5M5
• Cytogenetics: 15q25.1
• Protein Families: Transmembrane
• MW: 125.7 kDa
• Gene Summary: Probable ion channel.[UniProtKB/Swiss-Prot Function]