TBCE (NM_001079515) Human Untagged Clone

CAT#: SC315584

TBCE (untagged)-Human tubulin folding cofactor E (TBCE), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TBCE
• Synonyms: HRD; KCS; KCS1; pac2; PEAMO
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC315584 representing NM_001079515.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGAGTGACACTTTGACAGCGGATGTCATTGGTCGAAGAGTTGAAGTTAATGGAGAACATGCAACAGTA
  CGTTTTGCTGGTGTTGTCCCTCCCGTGGCAGGACCCTGGTTAGGAGTAGAATGGGACAATCCCGAGAGA
  GGAAAGCATGATGGGAGCCACGAAGGGACTGTGTATTTTAAATGCAGGCACCCGACAGGAGGATCCTTT
  ATTCGTCCGAACAAGGTAAATTTTGGAACAGACTTTCTTACTGCAATTAAGAACCGCTATGTGTTAGAA
  GATGGACCAGAGGAAGATAGAAAAGAGCAAATTGTTACAATTGGAAATAAACCTGTGGAGACTATCGGT
  TTTGACTCTATTATGAAACAGCAAAGTCAGCTGAGCAAGTTGCAAGAAGTTTCTCTGAGGAACTGTGCA
  GTAAGTTGTGCTGGTGAAAAAGGAGGAGTTGCTGAAGCATGTCCTAATATCAGAAAGGTAGATTTGTCA
  AAAAACCTGTTGTCATCATGGGATGAAGTGATACACATTGCTGATCAGCTCAGACACCTGGAAGTCCTT
  AATGTCAGTGAAAATAAACTAAAATTTCCCTCCGGTTCAGTATTAACTGGAACGCTTTCTGTACTGAAG
  GTTTTAGTCCTCAATCAAACAGGAATAACGTGGGCTGAGGTGCTGCGGTGTGTCGCGGGGTGCCCAGGC
  CTGGAGGAACTCTACCTTGAGTCTAACAACATTTTCATTTCCGAAAGGCCAACAGATGTTCTCCAGACA
  GTCAAGTTATTAGATCTTTCCTCTAATCAATTAATTGATGAAAATCAGCTGTATCTGATAGCCCACCTG
  CCCAGGTTAGAACAATTAATCCTCTCTGACACTGGAATTTCTTCTCTACATTTTCCGGATGCTGGAATT
  GGGTGCAAAACGTCCATGTTCCCATCCTTGAAGTACCTGGTAGTAAACGACAATCAGATATCACAATGG
  TCGTTTTTCAATGAGCTAGAGAAGTTACCAAGTCTACGGGCTTTGTCCTGCCTAAGAAACCCCCTGACC
  AAAGAGGACAAAGAAGCAGAGACGGCGCGACTACTCATTATCGCCAGCATTGGCCAGCTGAAGACGCTG
  AACAAATGTGAGATTCTCCCCGAGGAGAGGCGGAGAGCTGAGCTTGACTACCGAAAAGCTTTTGGAAAT
  GAGTGGAAACAGGCTGGTGGACATAAGGATCCGGAAAAAAACAGACTCAGCGAAGAATTCCTCACAGCC
  CATCCCAGATACCAGTTCCTCTGCCTGAAATATGGTGCACCTGAAGATTGGGAACTCAAAACACAGCAA
  CCACTTATGCTGAAAAACCAGCTACTAACACTGAAGATAAAATACCCTCATCAACTTGATCAGAAAGTC
  CTGGAGAAACAACTGCCGGGCTCCATGACAATTCAAAAGGTGAAGGGATTGCTGTCACGTCTTCTCAAA
  GTTCCTGTGTCAGACCTTCTGTTGTCCTATGAAAGTCCCAAAAAGCCGGGCAGAGAAATCGAGCTGGAA
  AATGACCTAAAGTCATTACAGTTTTATTCTGTGGAAAATGGAGATTGTCTATTAGTGCGATGGTGA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001079515
• Insert Size: 1584 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001079515.2
• RefSeq Size: 2025 bp
• RefSeq ORF: 1584 bp
• Locus ID: 6905
• UniProt ID: Q15813
• Cytogenetics: 1q42.3
• Protein Families: Druggable Genome
• MW: 59.3 kDa
• Gene Summary: Cofactor E is one of four proteins (cofactors A, D, E, and C) involved in the pathway leading to correctly folded beta-tubulin from folding intermediates. Cofactors A and D are believed to play a role in capturing and stabilizing beta-tubulin intermediates in a quasi-native confirmation. Cofactor E binds to the cofactor D/beta-tubulin complex; interaction with cofactor C then causes the release of beta-tubulin polypeptides that are committed to the native state. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) encodes isoform (a). Both variants 1 and 2 encode the same isoform.