DIAPH2 (NM_007309) Human Untagged Clone

CAT#: SC315175

DIAPH2 (untagged)-Human diaphanous homolog 2 (Drosophila) (DIAPH2), transcript variant 12C

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DIAPH2
• Synonyms: DIA; DIA2; DRF2; POF; POF2; POF2A
• Vector: pCMV6 series
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_007309, the custom clone sequence may differ by one or more nucleotides
  ATGGAGCAGCCCGGGGCGGCGGCGTCGGGAGCGGGAGGCGGCAGCGAGGAACCCGGTGGG
  GGCCGGAGCAACAAGCGGAGCGCGGGGAACCGGGCCGCCAATGAAGAGGAAACGAAAAAC
  AAACCCAAATTGAACATTCAAATAAAAACTTTGGCAGATGATGTGCGTGACCGAATTACA
  AGTTTTAGAAAATCTACTGTCAAAAAAGAAAAACCTCTTATTCAACATCCTATTGATTCT
  CAAGTCGCGATGAGTGAGTTTCCTGCAGCTCAGCCATTATATGATGAACGATCTTTGAAT
  TTGTCAGAAAAGGAAGTATTGGATCTCTTTGAAAAAATGATGGAGGACATGAACCTTAAC
  GAAGAGAAAAAAGCTCCTTTACGAAACAAAGACTTTACCACCAAACGTGAGATGGTTGTC
  CAGTATATTTCTGCCACTGCCAAATCTGGTGGGCTGAAAAACAGCAAACATGAATGCACC
  CTGTCTTCACAAGAATATGTTCATGAATTACGATCGGGTATATCAGATGAGAAACTTCTT
  AATTGCCTAGAATCCCTCAGGGTTTCTTTAACCAGCAATCCGGTCAGCTGGGTTAACAAC
  TTTGGCCATGAAGGTCTTGGACTCTTATTGGATGAGCTGGAAAAGCTTCTGGACAAAAAA
  CAGCAAGAAAATATTGACAAGAAGAATCAGTATAAACTTATTCAATGCCTCAAAGCATTT
  ATGAATAATAAGTTTGGATTACAAAGGATTCTAGGAGATGAAAGAAGTCTTTTACTATTG
  GCAAGAGCAATTGACCCCAAACAACCCAACATGATGACTGAAATAGTAAAAATACTTTCT
  GCTATTTGCATTGTTGGAGAAGAGAACATTCTAGATAAACTTTTAGGGGCTATAACAACA
  GCAGCAGAAAGAAATAACAGGGAACGATTTTCACCAATTGTGGAAGGTTTAGAAAATCAG
  GAAGCCTTGCAATTACAGGTGGCCTGCATGCAGTTTATAAATGCCCTTGTCACTTCTCCT
  TATGAGCTTGATTTTCGAATACATTTAAGGAATGAATTCCTCCGTTCAGGACTAAAAACA
  ATGTTACCAGATCTAAAAGAAAAAGAGAATGATGAGCTTGATATTCAGTTGAAAGTATTT
  GATGAAAACAAAGAAGATGACCTAACTGAATTATCACACCGTCTCAATGACATTCGAGCA
  GAAATGGATGATATGAATGAAGTCTACCATCTTCTATATAATATGCTGAAGGACACTGCT
  GCTGAAAATTACTTCTTATCTATTCTACAACATTTTTTGCTTATCAGAAATGATTATTAT
  ATCAGGCCACAATATTATAAAATAATTGAGGAATGTGTTTCACAGATAGTGCTACACTGC
  AGTGGTATGGATCCAGACTTCAAATACAGGCAAAGATTAGACATCGATTTAACTCATCTG
  ATAGATTCTTGTGTGAACAAGGCGAAAGTTGAAGAAAGTGAACAAAAAGCTGCAGAGTTT
  TCAAAGAAGTTCGATGAAGAATTCACAGCTCGACAGGAAGCTCAAGCAGAGCTTCAAAAA
  AGAGATGAGAAAATCAAAGAACTTGAAGCAGAAATCCAGCAACTTCGAACCCAGGCACAA
  GTACTCTCAAGTTCATCAGGAATTCCAGGTCCTCCTGCAGCACCTCCATTGCCAGGTGTA
  GGGCCGCCTCCACCACCACCCGCGCCACCTCTACCCGGAGGAGCTCCTCTTCCTCCTCCA
  CCACCTCCTTTACCTGGAATGATGGGGATACCACCACCACCCCCACCACCACTTTTATTT
  GGGGGACCTCCTCCACCACCACCCCTTGGAGGAGTTCCTCCTCCCCCAGGAATATCACTT
  AATCTACCTTATGGAATGAAGCAGAAAAAAATGTATAAACCTGAAGTGTCCATGAAGAGA
  ATCAATTGGTCAAAGATTGAACCCACAGAATTATCTGAGAACTGTTTCTGGTTAAGAGTC
  AAAGAAGACAAGTTTGAGAATCCAGATCTCTTTGCCAAATTGGCATTGAATTTTGCTACT
  CAGATAAAAGTTCAAAAGAACGCAGAAGCATTAGAAGAAAAGAAGACTGGGCCTACAAAG
  AAGAAAGTGAAAGAACTGAGAATTTTGGATCCCAAAACAGCTCAGAATCTGTCCATCTTT
  CTGGGATCATATCGCATGCCATATGAAGACATAAGAAACGTTATTCTGGAGGTTAATGAA
  GACATGCTGAGTGAGGCTTTAATTCAGAACCTTGTGAAACATCTTCCTGAGCAGAAGATA
  CTCAACGAATTAGCAGAGCTTAAGAATGAATATGATGACCTCTGTGAGCCTGAACAATTT
  GGAGTTGTGATGAGCTCTGTGAAAATGTTACAGCCTCGTCTCAGTAGTATCCTGTTCAAG
  CTCACATTTGAAGAACACATAAACAACATCAAACCAAGCATCATAGCAGTAACTCTTGCC
  TGTGAAGAACTGAAGAAAAGTGAAAGCTTTAACAGACTTTTAGAGTTAGTTCTTCTTGTT
  GGAAACTACATGAACTCAGGCTCAAGAAATGCCCAGTCTTTGGGATTTAAGATCAACTTC
  CTTTGTAAGATCAGAGATACTAAATCAGCGGATCAAAAAACAACCCTTTTGCATTTTATT
  GCCGACATTTGTGAGGAAAAATATCGAGATATCCTAAAATTTCCTGAAGAACTGGAACAC
  GTAGAAAGTGCAAGCAAAGTTTCAGCTCAAATTCTCAAGAGCAACCTTGCATCAATGGAA
  CAACAAATTGTTCATCTGGAACGTGACATCAAGAAATTCCCCCAAGCAGAAAATCAACAC
  GATAAGTTTGTGGAAAAGATGACCAGCTTTACAAAGACTGCCCGAGAACAGTATGAAAAA
  CTCTCCACCATGCACAACAACATGATGAAGCTCTATGAGAATCTTGGAGAATACTTCATT
  TTTGACTCAAAGACAGTGAGCATAGAAGAGTTCTTTGGTGATCTCAACAACTTCCGAACT
  TTGTTTTTGGAAGCAGTGAGAGAAAACAATAAGAGAAGAGAAATGGAAGAGAAGACCAGG
  AGGGCAAAACTTGCAAAAGAGAAAGCTGAACAAGAAAAGTTAGAACGCCAGAAGAAAAAG
  AAACAACTCATTGATATAAACAAAGAGGGTGATGAGACTGGTGTGATGGATAATCTTCTA
  GAAGCCCTACAATCAGGTGCAGCATTCAGAGACCGTCGAAAGCGGATTCCAAGGAATCCA
  GTGGTAAATCATCCCTGTGCAACAAGGGCTAATCCAAGATCAGCTACA
  
• Restriction Sites: Please inquire
• ACCN: NM_007309
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007309.1, NP_009293.1
• RefSeq Size: 3753 bp
• RefSeq ORF: 3291 bp
• Locus ID: 1730
• UniProt ID: O60879
• Cytogenetics: Xq21.33
• Domains: FH2
• Protein Families: Druggable Genome
• Protein Pathways: Regulation of actin cytoskeleton
• Gene Summary: The product of this gene belongs to the diaphanous subfamily of the formin homology family of proteins. This gene may play a role in the development and normal function of the ovaries. Defects in this gene have been linked to premature ovarian failure 2. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (12C) differs in the 3' UTR and the 3' coding region, compared to variant 156. The resulting isoform (isoform 12C) contains a distinct C-terminus, compared to isoform 156.