MMS19 (NM_022362) Human Untagged Clone

CAT#: SC315090

MMS19 (untagged)-Human MMS19 nucleotide excision repair homolog (S. cerevisiae) (MMS19)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MMS19
• Synonyms: CIAO4; hMMS19; MET18; MMS19L
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_022362 edited
  GAGGCGCATGCGCGGAGAGCCCGTCTCGAGCCACCGCCCATATCCCCTCCCACGGTCTCT
  AGTTCGCGTTATGGCCGCTGCCGCGGCTGTGGAGGCGGCGGCGCCTATGGGTGCCCTATG
  GGGCCTCGTGCACGACTTCGTCGTGGGTCAGCAAGAGGGCCCCGCTGACCAGGTGGCTGC
  AGATGTGAAATCTGGCAACTATACAGTGTTACAAGTTGTGGAAGCCCTTGGGTCCTCTCT
  AGAGAATCCAGAACCCCGAACTCGGGCACGAGGAATCCAGCTTTTGTCACAGGTGCTACT
  CCACTGTCACACCTTGCTCCTGGAGAAGGAAGTGGTACACCTGATACTGTTCTATGAGAA
  CCGGCTGAAGGACCATCATCTTGTGATCCCATCTGTCCTGCAGGGTTTGAAGGCACTTAG
  CCTGTGTGTGGCCCTGCCCCCAGGGCTGGCTGTTTCTGTGCTTAAAGCCATCTTCCAGGA
  AGTGCATGTACAGTCCCTGCCACAGGTGGACCGACACACAGTCTACAATATCATCACCAA
  TTTTATGCGAACCCGGGAAGAAGAGCTAAAGAGCCTAGGAGCTGACTTCACCTTTGGCTT
  CATCCAGGTGATGGATGGGGAAAAGGATCCCCGTAATCTTCTGGTGGCCTTCCGCATCGT
  CCATGACCTCATCTCCAGGGACTATAGCCTGGGACCCTTTGTGGAGGAGTTGTTTGAAGT
  GACATCCTGTTATTTCCCTATCGATTTTACCCCTCCACCTAATGATCCCCATGGTATCCA
  GAGAGAAGACCTCATCCTGAGTCTTCGCGCTGTGCTGGCTTCTACACCACGATTTGCTGA
  GTTTCTGCTGCCCCTGTTGATTGAGAAAGTGGATTCTGAGGTTCTGAGTGCCAAGTTGGA
  TTCTCTACAGACTCTGAATGCTTGCTGTGCTGTGTATGGACAGAAGGAACTGAAGGACTT
  CCTCCCCAGCCTTTGGGCTTCTATCCGCAGAGAGGTGTTCCAGACGGCAAGTGAGCGGGT
  GGAGGCAGAGGGCCTGGCGGCCCTCCACTCCCTGACTGCGTGTTTGTCTCGCTCTGTGCT
  GAGGGCTGATGCTGAGGACCTCCTTGACTCCTTCCTTAGCAACATTCTACAGGACTGCAG
  GCACCACCTGTGTGAACCGGACATGAAACTGGTGTGGCCTAGTGCCAAGCTGTTGCAGGC
  AGCTGCAGGTGCATCTGCCCGGGCCTGTGACTCTGTCACCAGCAATGTACTGCCTTTACT
  GCTGGAACAGTTCCACAAGCACAGTCAGAGCAGCCAGCGGCGGACAATCCTTGAAATGCT
  CCTGGGTTTCTTGAAGCTGCAGCAGAAATGGAGCTATGAAGACAAAGATCAAAGGCCTCT
  GAATGGCTTCAAGGACCAGCTGTGCTCACTGGTATTCATGGCTCTAACAGACCCCAGCAC
  CCAGCTTCAGCTTGTTGGCATCCGTACACTGACAGTCTTGGGTGCCCAGCCAGATCTCCT
  ATCTTATGAGGACTTGGAGCTGGCAGTGGGTCACCTGTACAGACTGAGCTTCCTGAAGGA
  GGATTCCCAGAGTTGCAGGGTGGCAGCACTGGAAGCATCAGGAACCCTGGCTGCTCTCTA
  CCCTGTGGCCTTCAGCAGCCACCTCGTACCCAAGCTCGCTGAGGAGCTGCGTGTAGGGGA
  GTCAAATTTGACTAACGGAGATGAGCCCACCCAATGCTCCCGGCATCTGTGCTGTCTGCA
  AGCCTTGTCAGCTGTATCAACACATCCCAGCATCGTCAAGGAGACACTGCCTCTGCTGCT
  GCAGCATCTCTGGCAAGTGAACAGAGGGAATATGGTTGCACAATCCAGTGACGTTATTGC
  TGTCTGTCAGAGCCTCAGACAGATGGCAGAAAAATGTCAGCAGGACCCTGAGAGTTGCTG
  GTATTTCCACCAGACAGCTATACCTTGCCTGCTTGCCTTGGCTGTGCAGGCCTCTATGCC
  AGAGAAGGAGCCCTCAGTTCTGAGAAAAGTACTATTGGAGGATGAGGTGTTGGCTGCCAT
  GGTGTCTGTCATTGGCACTGCTACAACCCACCTGAGCCCTGAGTTAGCTGCCCAGAGTGT
  GACACACATTGTGCCCCTCTTCTTGGATGGCAACGTGTCCTTTCTGCCTGAAAACAGCTT
  CCCGAGCAGATTCCAGCCATTCCAGGATGGCTCCTCAGGGCAGAGGCGGCTGATTGCACT
  GCTTATGGCCTTTGTCTGCTCCCTGCCTCGAAATGTGGAAATCCCTCAGCTGAACCAACT
  CATGCGGGAGCTTTTGGAACTGAGCTGCTGCCACAGCTGCCCCTTTTCTTCCACCGCTGC
  TGCCAAGTGCTTTGCAGGACTCCTCAACAAGCACCCTGCAGGGCAGCAGCTGGATGAATT
  CCTACAGCTAGCTGTGGACAAAGTGGAGGCTGGCCTGGACTCTGGGCCCTGTCGTAGTCA
  GGCCTTCACTCTTCTTCTCTGGGTAACAAAGGCCCTAGTGCTCAGATACCATCCTCTCAG
  CTCCTGCCTTACAGCCCGGCTCATGGGCCTCCTGAGTGACCCAGAATTAGGTCCAGCAGC
  AGCTGATGGCTTCTCTCTGCTCATGTCTGACTGCACTGATGTGCTGACTCGTGCTGGCCA
  TGCCGAAGTGCGGATCATGTTCCGCCAGCGGTTCTTCACAGATAATGTGCCTGCTTTGGT
  CCAGGGCTTCCATGCTGCTCCCCAAGATGTGAAGCCAAACTACTTGAAGGGTCTTTCTCA
  TGTACTTAACAGGCTGCCCAAGCCTGTACTCTTGCCAGAGCTGCCCACGCTTCTTTCCTT
  GCTGCTGGAGGCCCTGTCCTGCCCTGACTGTGTGGTGCAGCTCTCCACCCTCAGCTGCCT
  TCAGCCTCTTCTACTGGAAGCACCCCAAGTCATGAGTCTTCACGTGGACACCCTCGTCAC
  CAAGTTTCTGAACCTCAGCTCTAGCCCTTCCATGGCTGTCCGGATCGCCGCACTGCAGTG
  CATGCATGCTCTCACTCGCCTGCCCACCCCTGTGCTGCTGCCGTACAAACCACAGGTGAT
  TCGGGCCTTAGCCAAACCCCTGGATGACAAGAAGAGACTGGTGCGCAAGGAAGCAGTGTC
  AGCCAGAGGGGAGTGGTTTCTGTTGGGGAGCCCTGGCAGCTGAGCCCTCAGTCCTGGCCT
  AGACTGTTCTGACAATCTAACCTGGGATTACTAACTGTTGAGCCATCTTCCCCAAAGCAG
  GGAAACCACTGGTCTCTGACTGCCTTTCCCACAGACACAGCACAAATGCTAGGCCTCTGT
  TGCATGGCTGTACAAAGAACATAAGAGTCCATATTTCTAGTGGATTTGTAAAATAAGTGT
  GTGTGAGACACTTGCGTTTGAAGAAAGATCTAGGGTCCTGGGTCTCTTGCATTTATATGT
  CAGAAAAGGGGCGATATGCTGCTGAGGGGTGAGTGCATATGAGTGTGGCCCTGAGGACCA
  GGGCTGGCAGATGTTGTCTACCTGCTGAAGAATAAAGATTTCTTTTGGTAAAAAAAAAAA
  AAAAAAAAAA
  
• Restriction Sites: Please inquire
• ACCN: NM_022362
• Insert Size: 3550 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The ORF of this clone has been fully sequenced and found to contain one SNP compared with NM_022362.2. The SNP is a valid as published.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022362.2, NP_071757.2
• RefSeq Size: 3504 bp
• RefSeq ORF: 3093 bp
• Locus ID: 64210
• UniProt ID: Q96T76
• Cytogenetics: 10q24.1
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: Key component of the cytosolic iron-sulfur protein assembly (CIA) complex, a multiprotein complex that mediates the incorporation of iron-sulfur cluster into apoproteins specifically involved in DNA metabolism and genomic integrity. In the CIA complex, MMS19 acts as an adapter between early-acting CIA components and a subset of cellular target iron-sulfur proteins such as ERCC2/XPD, FANCJ and RTEL1, thereby playing a key role in nucleotide excision repair (NER), homologous recombination-mediated double-strand break DNA repair, DNA replication and RNA polymerase II (POL II) transcription (PubMed:22678362, PubMed:22678361, PubMed:29225034, PubMed:23585563). As part of the mitotic spindle-associated MMXD complex, plays a role in chromosome segregation, probably by facilitating iron-sulfur cluster assembly into ERCC2/XPD (PubMed:20797633). Indirectly acts as a transcriptional coactivator of estrogen receptor (ER), via its role in iron-sulfur insertion into some component of the TFIIH-machinery (PubMed:11279242).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes isoform 1. Variants 1 and 4 encode the same protein. Sequence Note: A downstream start codon is selected for this RefSeq based on better conservation with homologous proteins. The use of an alternative upstream start codon, which is present in some species, including human, chimp and macaque (with a subsequent CDS frameshift in the latter species), would increase the protein length from 1030 aa to 1051 aa. The shorter protein is referred to in the literature, including PMIDs 11071939, 11279242 and 11328871.