VPS50 (NM_017667) Human Untagged Clone

CAT#: SC315007

CCDC132 (untagged)-Human coiled-coil domain containing 132 (CCDC132), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: VPS50
• Synonyms: CCDC132; VPS54L
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC315007 representing NM_017667.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGCAAAAAATCAAATCTCTCATGACCCGACAGGGTCTGAAAAGCCCTCAAGAAAGCCTCAGTGATCTT
  GGTGCCATAGAGAGTCTCCGGGTCCCTGGAAAGGAAGAATTCAGGGAACTTCGAGAACAGCCAAGTGAC
  CCTCAAGCTGAACAAGAGCTTATTAATAGTATTGAACAAGTATATTTTTCTGTGGATTCATTTGATATT
  GTTAAATATGAGCTGGAGAAGCTTCCACCTGTTCTCAATTTGCAAGAATTAGAGGCGTATAGAGACAAA
  TTGAAACAACAGCAAGCTGCAGTATCTAAAAAAGTGGCAGATTTAATCCTTGAAAAACAGCCTGCTTAT
  GTAAAGGAACTTGAAAGAGTTACCTCATTGCAGACAGGTCTTCAATTAGCTGCTGTTATCTGTACAAAT
  GGGAGAAGACACTTGAATATTGCAAAGGAAGGTTTTACTCAAGCTAGTTTAGGCCTTCTTGCAAATCAA
  AGGAAACGTCAGTTGCTGATTGGACTTCTGAAATCTCTGAGAACTATAAAAACATTGCAAAGAACAGAT
  GTACGGTTAAGTGAAATGCTGGAGGAGGAAGATTATCCAGGAGCTATTCAGTTGTGCCTTGAATGTCAA
  AAAGCTGCCAGCACTTTTAAACATTACAGTTGTATAAGTGAACTGAATTCAAAGCTGCAAGATACTTTG
  GAACAGATTGAGGAACAGCTGGACGTAGCTCTTTCCAAAATCTGCAAGAATTTTGACATTAACCATTAT
  ACCAAGGTTCAACAAGCTTATCGACTTCTTGGAAAAACACAGACAGCAATGGATCAACTTCATATGCAC
  TTCACCCAAGCCATTCACAACACCGTGTTTCAAGTTGTTCTTGGTTATGTGGAACTATGTGCAGGAAAC
  ACAGACACAAAATTCCAAAAGCTGCAATATAAGGATCTCTGTACACATGTTACACCAGACAGCTATATT
  CCATGCCTTGCAGACCTGTGCAAAGCACTATGGGAAGTTATGCTCAGCTATTATAGGACTATGGAATGG
  CATGAAAAGCATGACAATGAGGATACTGCTTCAGCTTCTGAAGGGAGTAATATGATAGGTACTGAAGAA
  ACTAATTTTGATCGTGGCTACATAAAAAAGAAATTAGAACATGGACTTACACGAATATGGCAGGATGTT
  CAGCTAAAAGTAAAAACCTACTTGCTTGGAACTGATTTGTCTATATTCAAATATGATGATTTCATCTTT
  GTTTTGGATATAATCAGCAGGTTGATGCAAGTTGGAGAAGAATTTTGTGGTAGCAAGTCTGAAGTTTTA
  CAGGAATCTATTAGAAAACAAAGTGTCAATTATTTCAAGAATTACCATAGAACACGGCTCGATGAACTG
  AGAATGTTCTTAGAGAATGAGACTTGGGAACTTTGTCCTGTTAAGTCAAATTTCAGCATCTTGCAACTT
  CATGAATTTAAATTCATGGAACAGTCTCGCTCCCCATCAGTTTCACCTAGTAAACAGCCAGTCTCAACT
  TCTTCAAAAACAGTGACCTTGTTTGAGCAGTACTGTAGTGGTGGGAATCCATTTGAAATTCAGGCCAAC
  CACAAAGATGAAGAAACAGAAGATGTCTTAGCTTCTAATGGGTATGAATCTGATGAACAAGAAAAGAGT
  GCCTATCAAGAGTATGACAGTGACAGTGATGTTCCTGAGGAACTCAAACGAGACTATGTGGATGAGCAG
  ACAGGAGATGGTCCTGTGAAAAGTGTTTCTCGGGAAACTCTAAAAAGCAGGAAGAAATCAGATTACAGT
  CTAAATAAAGTGAATGCACCTATCTTAACAAATACAACATTGAACGTCATAAGACTTGTTGGAAAATAT
  ATGCAGATGATGAACATTCTTAAGCCAATTGCCTTTGATGTTATTCATTTCATGTCTCAACTATTTGAT
  TATTACTTGTATGCAATATATACCTTTTTTGGTCGGAATGATTCATTGGAATCAACTGGACTCGGCCTT
  AGTAGTAGTAGACTAAGAACAACTCTAAACAGAATACAAGAAAGCCTTATTGATCTAGAAGTTTCAGCT
  GATCCTACTGCCACACTCACAGCAGCAGAAGAAAGAAAGGAGAAGGTGCCAAGTCCACACCTCAGTCAC
  CTAGTGGTTTTGACATCTGGGGATACGCTGTATGGGTTGGCAGAAAGAGTGGTAGCCACGGAATCCTTG
  GTATTCTTGGCTGAACAGTTTGAGTTCCTTCAGCCACATCTGGATGCTGTGATGCCTGCAGTCAAAAAG
  CCCTTTCTTCAGCAGTTCTATTCTCAGACAGTCTCAACCGCCAGTGAACTACGGAAACCAATTTACTGG
  ATTGTAGCTGGTAAAGCCCTTGATTATGAACAGATGCTGCTTCTCATGGCTAATGTGAAATGGGATGTA
  AAAGAAATTATGTCACAGCACAACATATATGTAGATGCACTATTAAAGGAATTTGAGCAGTTTAACAGG
  AGGCTAAATGAAGTTTCTAAGAGAGTTCGCATACCCTTGCCTGTGTCTAATATACTTTGGGAACATTGT
  ATACGATTGGCTAATCGAACTATTGTAGAAGGATATGCCAATGTCAAGAAATGCAGTAATGAGGGTCGT
  GCCCTGATGCAATTGGATTTTCAACAGTTTTTAATGAAACTTGAAAAACTAACAGATATTAGACCCATT
  CCTGATAAAGAATTTGTAGAAACTTATATTAAAGCTTATTACCTAACTGAGAATGACATGGAACGGTGG
  ATCAAAGAGCACAGGGAATATTCAACGAAGCAGCTGACCAATCTGGTGAATGTTTGCCTGGGATCCCAT
  ATCAATAAGAAAGCAAGACAAAAACTTCTAGCAGCTATAGATGATATAGACAGACCTAAAAGATAA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_017667
• Insert Size: 2895 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017667.3
• RefSeq Size: 5713 bp
• RefSeq ORF: 2895 bp
• Locus ID: 55610
• UniProt ID: Q96JG6
• Cytogenetics: 7q21.2-q21.3
• MW: 111.2 kDa
• Gene Summary: Acts as component of the EARP complex that is involved in endocytic recycling. The EARP complex associates with Rab4-positive endosomes and promotes recycling of internalized transferrin receptor (TFRC) to the plasma membrane. Within the EARP complex, required to tether the complex to recycling endosomes. Not involved in retrograde transport from early and late endosomes to the trans-Golgi network (TGN).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes the longest isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.