PCDHA8 (NM_018911) Human Untagged Clone

CAT#: SC314978

PCDHA8 (untagged)-Human protocadherin alpha 8 (PCDHA8), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PCDHA8
• Synonyms: PCDH-ALPHA8
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC314978 representing NM_018911.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGATTATCACTGGCGAGGAGAGCTGGGATCCTGGCGACTACTACTCTTGCTTCTGCTCCTCGCAGCC
  TGGAAGGTGGGGAGCGGCCAGCTCCACTACTCCGTCCCCGAGGAGGCCAAACACGGCACCTTCGTGGGC
  CGGATCGCGCAGGACCTGGGGCTGGAGCTGGCGGAGCTGGTGCCGCGCCTGTTCCGGGTGGCGTCCAAA
  AGACACCGGGACCTTCTGGAGGTAAGTCTGCAGAATGGCATTTTGTTTGTGAATTCTCGGATCGACCGC
  GAGGAGCTGTGCGGGCGGAGCGCGGAGTGCAGCATCCACCTGGAGGTGATCGTGGACAGGCCGCTGCAG
  GTTTTCCATGTGGACGTGGAGGTGAAGGATGTTAATGACAACCCGCCAGTGTTCCGGGTAAAAGACCAA
  AAGCTGTTTGTTTCAGAATCCAGAATGCCAGACTCTCGGTTTCCGCTAGAGGGCGCGTCCGATGCAGAT
  GTTGGAGCTAACTCCGTGTTAACCTACAGGCTTAGCTCTCATGATTACTTCATGCTAGATGTGAATTCA
  AAGAACGATGAGAATAAACTGGTTGAGCTCGTATTAAGAAAATCCTTGGACAGAGAGGACGCTCCTGCG
  CACCACTTATTCCTGACAGCCACAGATGGGGGCAAACCTGAGCTCACAGGCACTGTTCAGCTGCTGGTC
  ACAGTGCTGGATGTGAATGATAATGCTCCCACTTTCGAACAGTCTGAATACGAAGTAAGAATATTCGAA
  AACGCAGACAACGGAACAACAGTTATCAAACTGAATGCTTCTGATCCGGATGAAGGAGCCAATGGGGCA
  ATTTCATATTCTTTTAATAGCCTTGTTGAAACTATGGTTATTGACCACTTTAGCATAGATCGAAATACG
  GGAGAAATAGTGATTCGGGGTAATTTGGATTTTGAACAAGAAAACTTATACAAAATCCTCATTGACGCC
  ACGGACAAAGGCCATCCTCCCATGGCGGGTCATTGCACCGTTTTAGTGAGAATTTTGGATAAAAATGAT
  AACGTCCCTGAGATAGCACTGACTTCCTTATCCTTGCCTGTACGTGAAGACGCTCAATTTGGTACTGTC
  ATCGCCCTAATTAGCGTGAACGACCTCGATTCAGGTGCCAACGGGCAGGTGACCTGCTCCCTGATGCCC
  CATGTCCCCTTCAAGCTGGTGTCCACCTTCAAGAATTACTACTCGTTGGTGCTGGACAGCGCCCTGGAC
  CGCGAGAGAGTGTCGGCCTATGAGTTGGTGGTAACCGCGCGGGACGGGGGCTCGCCTTCGCTGTGGGCC
  ACCGCCAGCTTGTCTGTGGAGGTGGCCGACGTGAACGACAATGCTCCGGCGTTCGCGCAGCCCGAGTAC
  ACGGTGTTCGTGAAGGAGAACAACCCGCCGGGCTGCCACATCTTCACGGTGTCTGCGCGAGACGCGGAC
  GCGCAGGAGAACGCGCTGGTGTCCTACTCGCTTGTGGAGCGGCGGGTGGGCGAGCGCTCGCTGTCGAGC
  TACATTTCGGTGCACACGGAGAGCGGCAAGGTGTACGCGCTGCAGCCGCTGGACCACGAGGAGCTAGAG
  CTGCTGCAGTTCCAGGTGAGCGCGCGCGACGCGGGCGTGCCGCCTCTGGGCAGCAACGTGACGCTGCAG
  GTGTTCGTGCTGGACGAGAATGACAACGCGCCGGCACTGCTGGAGCCTCGGGTGGGTGGCACTGGTGGC
  GCAGCGAGCAAGCTGGTGCCGCGGTCTGTGGGCGCGGGCCACGTGGTAGCGAAGGTGCGCGCAGTGGAC
  GCCGACTCGGGCTACAACGCGTGGCTTTCGTATGAGCTGCAGCCAGCTGCAAGCAGCCCTCGCATCCCG
  TTCCGCGTGGGGCTGTACACGGGCGAGATCAGCACCACTCGTGTCCTGGACGAAGCGGACTCTCCGCGC
  CACCGTCTGCTGGTCCTGGTGAAGGATCATGGTGAACCTGCGCTGACCGCCACGGCCACGGTTCTGGTG
  TCGCTGGTGGAGAGCGGCCAGGCTCCAAAAGCGTCATCGAGGCAGTCGGCTGGCGTTTTGGGTCCGGAA
  GCGGCGCTGGTGGATGTCAACGTGTACCTGATCATCGCCATCTGCGCGGTATCCAGCCTGCTGGTGCTC
  ACGCTGCTGCTGTACACTGCGCTGCGGTGCTCAGCACTGCCCACTGAGGGCGGGTGCCGGGCGGGCAAG
  CCCACTCTGGTGTGCTCCAGTGCGGTGGGGAGCTGGTCATACTCGCAACAACAGCCGCAGAGGGTGTGC
  TCTGGTGAGGGGCCACCGAAGACGGACCTCATGGCCTTCAGCCCCTGCCTTCCTCCTGATCTGGGATCA
  GTTGATGTAGGCGAAGAGCAAGATTTAAATGTTGATCATGGCCTCAAACCACGACAGCCCAACCCTGAC
  TGGCGTTACTCTGCCTCCCTGAGAGCAGGCATGCACAGCTCTGTGCACCTAGAGGAGGCTGGCATTCTA
  CGGGCTGGTCCAGGAGGGCCTGATCAGCAGTGGCCAACAGTATCCAGTGCAACACCAGAACCAGAGGCA
  GGAGAAGTGTCCCCTCCAGTCGGTGCGGGTGTCAACAGCAACAGCTGGACCTTTAAATACGGACCAGGC
  AACCCCAAACAATCCGGTCCCGGTGAGTTGCCCGACAAATTCATTATCCCAGGATCTCCTGCAATCATC
  TCCATCCGGCAGGAGCCTACTAACAGCCAAATTGACAAAAGTGACTTCATAACCTTCGGCAAAAAGGAG
  GAGACCAAGAAAAAGAAGAAAAAGAAGAAGGGTAACAAGACCCAGGAGAAAAAAGAGAAAGGGAACAGC
  ACGACTGACAACAGTGACCAGTGA
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGAT
  ATCCTGGATTACAAGGATGACGACGATAAGGTTTAA
  
• Restriction Sites: SgfI-RsrII
• ACCN: NM_018911
• Insert Size: 2853 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018911.2
• RefSeq Size: 5260 bp
• RefSeq ORF: 2853 bp
• Locus ID: 56140
• UniProt ID: Q9Y5H6
• Cytogenetics: 5q31.3
• Domains: CA
• Protein Families: Transmembrane
• MW: 103.1 kDa
• Gene Summary: This gene is a member of the protocadherin alpha gene cluster, one of three related gene clusters tandemly linked on chromosome five that demonstrate an unusual genomic organization similar to that of B-cell and T-cell receptor gene clusters. The alpha gene cluster is composed of 15 cadherin superfamily genes related to the mouse CNR genes and consists of 13 highly similar and 2 more distantly related coding sequences. The tandem array of 15 N-terminal exons, or variable exons, are followed by downstream C-terminal exons, or constant exons, which are shared by all genes in the cluster. The large, uninterrupted N-terminal exons each encode six cadherin ectodomains while the C-terminal exons encode the cytoplasmic domain. These neural cadherin-like cell adhesion proteins are integral plasma membrane proteins that most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. Alternative splicing has been observed and additional variants have been suggested but their full-length nature has yet to be determined. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) includes the constant region exons and encodes the longest isoform (1).