BICD2 (NM_015250) Human Untagged Clone

CAT#: SC314779

BICD2 (untagged)-Human bicaudal D homolog 2 (Drosophila) (BICD2), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: BICD2
• Synonyms: bA526D8.1; SMALED2; SMALED2A; SMALED2B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC314779 representing NM_015250.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGTCGGCGCCGTCGGAGGAGGAGGAGTACGCGCGGCTGGTGATGGAGGCGCAGCCGGAGTGGCTGCGC
  GCCGAGGTGAAGCGGCTGTCCCACGAGCTGGCCGAGACCACGCGTGAGAAGATCCAGGCGGCCGAGTAC
  GGGCTGGCGGTGCTCGAGGAGAAGCACCAGCTCAAGCTGCAGTTCGAGGAGCTCGAGGTGGACTATGAG
  GCTATCCGCAGCGAGATGGAGCAGCTCAAGGAGGCCTTTGGACAAGCACACACAAACCACAAGAAGGTG
  GCTGCTGACGGAGAGAGCCGGGAGGAGAGCCTGATCCAGGAGTCGGCCTCCAAGGAGCAGTACTACGTG
  CGGAAGGTGCTAGAGCTGCAGACGGAGCTGAAGCAGTTGCGCAATGTCCTCACCAACACGCAGTCGGAG
  AATGAGCGCCTGGCCTCTGTGGCCCAGGAGCTGAAGGAGATCAACCAGAATGTGGAGATCCAGCGTGGC
  CGCCTGCGGGATGACATCAAGGAGTACAAATTCCGGGAAGCTCGTCTGCTGCAGGACTACTCGGAACTG
  GAGGAGGAGAACATCAGCCTGCAGAAGCAAGTGTCTGTGCTCAGACAGAACCAGGTGGAGTTTGAGGGC
  CTCAAGCATGAGATCAAGCGTCTGGAGGAGGAGACCGAGTACCTCAACAGCCAGCTGGAGGATGCCATC
  CGCCTCAAGGAGATCTCAGAGCGGCAGCTGGAGGAGGCGCTGGAGACCCTGAAGACGGAGCGCGAACAG
  AAGAACAGCCTGCGCAAGGAGCTGTCACACTACATGAGCATCAATGACTCCTTCTACACCAGCCACCTG
  CATGTCTCGCTGGATGGCCTCAAGTTCAGTGACGATGCTGCCGAGCCCAACAACGATGCCGAGGCCCTG
  GTCAATGGCTTTGAGCACGGCGGCCTGGCCAAGCTGCCACTGGACAACAAGACCTCCACGCCCAAGAAG
  GAGGGCCTCGCACCGCCCTCCCCCAGCCTCGTCTCCGACCTACTCAGTGAGCTCAACATCTCTGAGATC
  CAGAAGCTGAAGCAGCAGCTGATGCAGATGGAGCGGGAAAAGGCGGGCCTGCTGGCAACGCTGCAGGAC
  ACACAGAAGCAGCTGGAGCACACGCGGGGCTCCCTGTCAGAACAGCAGGAGAAGGTGACCCGCCTCACA
  GAGAATCTGAGTGCCCTGCGGCGCCTGCAGGCCAGCAAGGAGCGGCAGACAGCCCTGGACAACGAGAAG
  GACCGTGACAGCCATGAGGATGGGGACTACTACGAGGTGGACATCAACGGGCCTGAGATCTTGGCCTGC
  AAGTACCATGTGGCTGTGGCTGAGGCTGGCGAGCTCCGCGAGCAGCTCAAGGCACTGCGCAGCACGCAC
  GAGGCTCGTGAGGCCCAGCACGCCGAGGAGAAGGGCCGCTATGAGGCTGAGGGCCAGGCACTCACGGAG
  AAGGTCTCCCTGCTAGAGAAGGCCAGCCGCCAGGACCGCGAGCTGCTGGCCCGGCTGGAGAAGGAGCTA
  AAGAAGGTGAGCGACGTCGCCGGCGAGACACAGGGCAGCCTGAGTGTGGCCCAGGATGAGCTGGTGACC
  TTCAGTGAGGAGCTGGCCAATCTCTACCACCACGTGTGCATGTGCAACAATGAGACACCCAACCGTGTC
  ATGCTGGACTACTACCGCGAGGGCCAGGGCGGGGCCGGCCGCACCAGTCCCGGGGGCCGCACCAGCCCC
  GAGGCGCGTGGCCGGCGCTCACCCATCCTCCTACCCAAGGGGCTGCTGGCTCCTGAGGCGGGCCGAGCA
  GATGGTGGGACGGGGGACAGCAGCCCCTCGCCTGGCTCCTCACTGCCATCACCCCTGAGTGACCCACGC
  CGGGAGCCCATGAACATCTACAACCTGATCGCTATCATCCGTGACCAGATCAAGCACCTGCAGGCAGCC
  GTGGACCGCACCACGGAGCTGTCACGCCAGCGCATTGCCTCTCAGGAGCTGGGCCCCGCCGTGGACAAG
  GACAAGGAAGCGCTTATGGAGGAGATCCTCAAGCTGAAGTCGCTGCTCAGCACCAAGCGGGAGCAGATC
  ACCACGCTGCGCACTGTGCTCAAGGCCAACAAGCAGACGGCCGAGGTGGCCCTTGCCAACCTGAAGAGC
  AAGTATGAGAATGAGAAGGCCATGGTTACCGAGACCATGATGAAGCTGCGCAATGAGCTCAAGGCCCTC
  AAGGAGGACGCAGCCACCTTCTCCTCGCTGCGTGCTATGTTTGCCACCAGGTGTGACGAGTACATTACA
  CAGCTGGATGAGATGCAGCGGCAGCTGGCGGCTGCTGAGGACGAGAAGAAGACGCTGAACTCGCTGCTG
  CGCATGGCCATCCAGCAGAAGCTGGCGCTGACCCAGCGGCTGGAGCTGCTCGAGCTGGACCATGAGCAG
  ACCCGGCGTGGCCGTGCCAAAGCCGCCCCGAAGACCAAGCCAGCCACACCGAGCCTGTAG
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGAT
  ATCCTGGATTACAAGGATGACGACGATAAGGTTTAA
  
• Restriction Sites: SgfI-RsrII
• ACCN: NM_015250
• Insert Size: 2475 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015250.3
• RefSeq Size: 4625 bp
• RefSeq ORF: 2475 bp
• Locus ID: 23299
• UniProt ID: Q8TD16
• Cytogenetics: 9q22.31
• MW: 93.5 kDa
• Gene Summary: This gene is one of two human homologs of Drosophila bicaudal-D and a member of the Bicoid family. It has been implicated in dynein-mediated, minus end-directed motility along microtubules. It has also been reported to be a phosphorylation target of NIMA related kinase 8. Two alternative splice variants have been described. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) encodes the shorter isoform (2). This variant includes an alternate intron, compared to variant 1, that causes a frameshift. The resulting protein (isoform 2) has a distinct C-terminus, compared to isoform 1.