EPS8L1 (NM_133180) Human Untagged Clone

CAT#: SC314543

EPS8L1 (untagged)-Human EPS8-like 1 (EPS8L1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: EPS8L1
• Synonyms: DRC3; EPS8R1; PP10566
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC314543 representing NM_133180.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGAGCACCGCCACAGGCCCAGAAGCTGCCCCAAAGCCAAGCGCCAAGTCTATCTATGAGCAGAGGAAG
  CGTTACTCCACAGTTGTTATGGCTGATGTATCCCAGTACCCAGTCAATCACCTGGTGACGTTCTGCCTG
  GGTGAGGACGATGGCGTGCATACCGTGGAGGATGCCTCCAGGAAGTTGGCCGTCATGGATAGCCAGGGC
  CGAGTCTGGGCACAGGAGATGCTGCTGCGAGTGTCTCCCGACCATGTCACGCTGCTCGACCCGGCCTCC
  AAGGAGGAGCTGGAGTCGTACCCACTGGGCGCCATCGTGCGCTGTGACGCGGTGATGCCACCCGGCAGG
  AGCCGCTCGTTGCTGCTGCTCGTGTGCCAGGAACCCGAGCGCGCGCAGCCCGACGTGCACTTCTTCCAG
  GGCCTGCGCCTCGGGGCGGAGCTGATCCGAGAGGACATCCAGGGGGCTCTGCACAATTACCGCTCGGGC
  CGCGGGGAGCGCAGGGCGGCGGCGCTCAGGGCCACGCAGGAGGAGTTGCAGCGCGACCGCTCGCCCGCC
  GCTGAGACCCCGCCCCTGCAGCGCCGCCCGTCAGTCCGCGCAGTGATCAGCACCGTAGAGCGGGGCGCG
  GGCCGCGGACGACCCCAGGCGAAGCCCATTCCCGAGGCAGAGGAGGCGCAGAGGCCTGAGCCGGTGGGG
  ACCTCGAGCAACGCTGACTCGGCCTCCCCGGACCTGGGTCCCCGGGGTCCTGACCTGGCGGTTCTGCAG
  GCGGAGCGGGAAGTGGACATCCTGAACCACGTGTTCGACGACGTAGAGAGCTTTGTATCGAGGCTGCAG
  AAGTCGGCGGAGGCGGCCAGGGTGCTGGAGCACCGGGAACGCGGCCGCAGGAGCCGGCGCCGGGCGGCT
  GGGGAGGGCTTGCTGACGCTGCGGGCCAAGCCGCCCTCGGAGGCCGAGTACACCGACGTGCTGCAGAAG
  ATCAAGTACGCCTTCAGCCTGCTGGCCCGGCTGCGCGGCAACATCGCCGACCCCTCCTCTCCGGAGCTG
  TTGCACTTCCTTTTCGGGCCTCTGCAGATGATTGTGAACACGTCGGGGGGGCCGGAGTTCGCGAGCAGT
  GTGCGGCGGCCGCATCTGACATCGGATGCCGTGGCGCTGCTGCGGGACAACGTCACTCCACGTGAAAAC
  GAGCTCTGGACCTCGCTGGGGGACTCGTGGACCCGCCCCGGGCTGGAGCTGTCCCCGGAGGAGGGACCC
  CCATACAGACCCGAGTTCTTCAGCGGCTGGGAGCCGCCGGTCACTGACCCGCAGAGCCGCGCCTGGGAG
  GACCCAGTTGAGAAACAGCTACAGCACGAGCGGAGGCGCCGGCAGCAAAGCGCCCCCCAGGTCGCTGTC
  AATGGTCACCGAGACTTGGAGCCAGAATCTGAGCCTCAGCTGGAGTCAGAGACAGCAGGAAAATGGGTC
  CTGTGTAATTATGACTTCCAGGCCCGCAACAGCAGTGAGCTGTCGGTCAAGCAGCGGGACGTACTGGAG
  GTCCTGGATGACAGTCGTAAGTGGTGGAAGGTTCGGGACCCAGCGGGGCAGGAGGGATATGTGCCCTAC
  AACATCCTGACACCCTACCCCGGACCCCGGCTGCACCACAGCCAAAGCCCTGCCCGCAGCCTGAACAGC
  ACTCCTCCTCCACCACCAGCCCCAGCCCCGGCCCCACCTCCAGCTCTGGCTCGGCCCCGCTGGGACAGG
  CCCCGCTGGGACAGCTGCGATAGCCTCAACGGCTTGGACCCCAGCGAGAAGGAGAAATTCTCCCAGATG
  CTCATCGTCAACGAGGAACTGCAGGCGCGCCTGGCCCAGGGCCGCTCGGGACCGAGCCGCGCAGTCCCA
  GGGCCCCGCGCCCCGGAACCGCAGCTCAGCCCGGGCTCGGACGCCTCCGAGGTCCGCGCCTGGCTGCAG
  GCCAAGGGCTTTAGCTCCGGGACCGTGGACGCGCTGGGTGTGCTGACCGGGGCGCAGCTTTTCTCGCTG
  CAGAAGGAGGAGCTGCGGGCGGTGAGCCCCGAGGAGGGGGCACGTGTGTACAGCCAGGTCACCGTGCAG
  CGCTCGCTGCTGGAGGACAAAGAGAAAGTGTCAGAGCTGGAGGCAGTGATGGAGAAGCAAAAGAAGAAG
  GTGGAAGGCGAGGTGGAAATGGAGGTCATTTGA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_133180
• Insert Size: 2172 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_133180.2
• RefSeq Size: 2607 bp
• RefSeq ORF: 2172 bp
• Locus ID: 54869
• UniProt ID: Q8TE68
• Cytogenetics: 19q13.42
• Domains: SH3, PID
• Protein Families: Druggable Genome
• MW: 80.3 kDa
• Gene Summary: This gene encodes a protein that is related to epidermal growth factor receptor pathway substrate 8 (EPS8), a substrate for the epidermal growth factor receptor. The function of this protein is unknown. At least two alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) encodes the longer isoform (a).