HECTD3 (AK056545) Human Untagged Clone

CAT#: SC314075

(untagged)-Human cDNA FLJ31983 fis, clone NT2RP7008557, weakly similar to DNA BINDING PROTEIN URE-B1 (EC 6.3.2.-)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HECTD3
• Vector: pCMV6 series
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for AK056545, the custom clone sequence may differ by one or more nucleotides
  
  
• Restriction Sites: Please inquire
• ACCN: AK056545
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.

Reference Data

• RefSeq: AK056545.1, BAG51742.1
• RefSeq Size: 2978 bp
• RefSeq ORF: 1734 bp
• Locus ID: 79654
• Cytogenetics: 1p34.1
• Domains: HECT
• Protein Families: Druggable Genome
• Gene Summary: The protein encoded by this gene transfers ubiquitin from an E2 ubiquitin-conjugating enzyme to targeted substrates, leading to the degradation of those substrates. The encoded protein has been shown to transfer ubiquitin to TRIOBP to facilitate cell cycle progression, and to STX8. [provided by RefSeq, Dec 2012]