OPALIN (NM_001040103) Human Untagged Clone

CAT#: SC310960

OPALIN (untagged)-Human oligodendrocytic myelin paranodal and inner loop protein (OPALIN), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: OPALIN
• Synonyms: HTMP10; TMEM10; TMP10
• Vector: pCMV6 series
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001040103, the custom clone sequence may differ by one or more nucleotides
  ATGCGGATGACGTCCTCTCCTGTCACAGGTGGGAAAGAAACGGACTGTGGGCCCTCTCTT
  GGATTAGCGGCGGGCATACCATTGCTGGTGGCCACAGCCCTGCTGGTGGCTTTACTATTT
  ACTTTGATTCACCGAAGAAGAAGCAGCATTGAGGCCATGGAGGAAAGTGACAGACCATGT
  GAAATTTCAGAAATTGATGACAATCCCAAGATATCTGAGAATCCTAGGAGATCACCCACA
  CATGAGAAGAATACGATGGGAGCACAAGAGGCCCACATATATGTGAAGACTGTAGCAGGA
  AGCGAGGAACCTGTGCATGACCGTTACCGTCCTACTATAGAAATGGAAAGAAGGAGGGGA
  TTGTGGTGGCTTGTGCCCAGACTGAGCCTGGAATGA
  
• Restriction Sites: Please inquire
• ACCN: NM_001040103
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001040103.1, NP_001035192.1
• RefSeq Size: 3629 bp
• RefSeq ORF: 396 bp
• Locus ID: 93377
• UniProt ID: Q96PE5
• Cytogenetics: 10q24.1
• Protein Families: Transmembrane
• Gene Summary: Central nervous system-specific myelin protein that increase myelin genes expression during oligodendrocyte differentiation. Promotes oligodendrocyte terminal differentiation.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) contains an alternate exon and uses an alternate start codon compared to variant 1. It encodes isoform c which is shorter and has a distinct N-terminus compared to isoform a. Variants 3, 6, 7 and 8 encode the same isoform (c).