LIMS2 (NM_017980) Human Untagged Clone

CAT#: SC310488

LIMS2 (untagged)-Human LIM and senescent cell antigen-like domains 2 (LIMS2), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: LIMS2
• Synonyms: LGMD2W; MDRCMTT; PINCH-2; PINCH2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC310488 representing NM_017980.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGCGGCCCGGCTGGGTGCGCTGGCCGCGTCGGGGCTGTACCGGCGGCGCCAGCACCGGCAGAGCCCG
  CCGCCAGCCACGGGCAATATGTCGGACGCCTTGGCCAACGCCGTGTGCCAGCGCTGCCAGGCCCGCTTC
  TCCCCCGCCGAGCGCATTGTCAACAGCAATGGGGAGCTGTACCATGAGCACTGCTTCGTGTGTGCCCAG
  TGCTTCCGGCCCTTCCCCGAGGGGCTCTTCTATGAGTTTGAAGGCCGGAAGTACTGCGAACACGACTTC
  CAAATGCTGTTTGCTCCGTGCTGTGGATCCTGCGGTGAGTTCATCATTGGCCGCGTCATCAAGGCCATG
  AACAACAACTGGCACCCGGGCTGCTTCCGCTGCGAGCTGTGTGATGTGGAGCTGGCTGACCTGGGCTTT
  GTGAAGAATGCCGGCAGGCATCTCTGCCGGCCTTGCCACAACCGTGAGAAGGCCAAGGGCCTGGGCAAG
  TACATCTGCCAGCGGTGCCACCTGGTCATCGACGAGCAGCCCCTCATGTTCAGGAGCGACGCCTACCAC
  CCTGACCACTTCAACTGCACCCACTGTGGGAAGGAGCTGACAGCCGAGGCCCGCGAGCTGAAGGGTGAG
  CTCTACTGCCTGCCCTGCCATGACAAGATGGGCGTCCCCATCTGCGGGGCCTGCCGCCGGCCCATCGAG
  GGCCGAGTGGTCAACGCGCTGGGCAAGCAGTGGCACGTGGAGCACTTTGTCTGTGCCAAGTGTGAGAAG
  CCATTCCTGGGGCACCGGCACTATGAGAAGAAGGGCCTGGCCTACTGCGAGACTCACTACAACCAGCTC
  TTCGGGGACGTCTGCTACAACTGCAGCCATGTGATTGAAGGCGATGTGGTGTCGGCCCTCAACAAGGCC
  TGGTGTGTGAGCTGCTTCTCCTGCTCCACCTGCAACAGCAAGCTCACCCTGAAGAACAAGTTTGTGGAG
  TTCGACATGAAGCCCGTGTGTAAGAGGTGCTACGAGAAGTTCCCGCTGGAGCTGAAGAAGCGGCTGAAG
  AAGCTGTCGGAGCTGACCTCCCGCAAGGCCCAGCCCAAGGCCACAGACCTCAACTCTGCCTGA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_017980
• Insert Size: 1098 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017980.4
• RefSeq Size: 2173 bp
• RefSeq ORF: 1098 bp
• Locus ID: 55679
• UniProt ID: Q7Z4I7
• Cytogenetics: 2q14.3
• Domains: LIM
• MW: 41.5 kDa
• Gene Summary: This gene encodes a member of a small family of focal adhesion proteins which interacts with ILK (integrin-linked kinase), a protein which effects protein-protein interactions with the extraceullar matrix. The encoded protein has five LIM domains, each domain forming two zinc fingers, which permit interactions which regulate cell shape and migration. A pseudogene of this gene is located on chromosome 4. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]
  Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (2) has a distinct N-terminus and is longer than isoform 1.