SSBP3 (NM_018070) Human Untagged Clone

CAT#: SC310480

SSBP3 (untagged)-Human single stranded DNA binding protein 3 (SSBP3), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SSBP3
• Synonyms: CSDP; SSDP; SSDP1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC310480 representing NM_018070.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGTTTGCCAAAGGCAAAGGCTCGGCGGTGCCCTCGGATGGGCAGGCTCGGGAAAAGTTAGCTTTATAC
  GTCTACGAATATTTACTGCACGTAGGAGCACAGAAATCTGCACAGACCTTCTTATCGGAGATTCGATGG
  GAAAAAAACATCACGTTGGGAGAACCGCCTGGGTTTTTGCACTCGTGGTGGTGTGTATTTTGGGACCTT
  TACTGTGCAGCTCCTGAAAGGAGAGACACTTGTGAACATTCAAGTGAAGCAAAAGCCTTTCATGATTAT
  AGTGCAGCAGCTGCCCCGAGCCCCGTGCTTGGCAACATTCCCCCCAACGATGGGATGCCGGGAGGCCCC
  ATCCCGCCAGGTTTCTTTCAGGGTCCTCCGGGGTCACAGCCCTCGCCGCACGCACAGCCTCCACCTCAC
  AATCCTAGCAGCATGATGGGACCCCACAGTCAGCCTCCGGGAGGAGTTCCTGGGACACAGCCATTGCTG
  CCCAATTCTATGGATCCCACACGACAACAAGGCCACCCCAACATGGGAGGATCAATGCAGAGAATGAAC
  CCTCCCCGAGGCATGGGGCCCATGGGTCCCGGCCCACAGAATTACGGCAGCGGCATGAGACCACCACCC
  AACTCCCTCGGCCCCGCCATGCCCGGGATTAACATGGGCCCGGGAGCTGGCAGACCCTGGCCCAATCCT
  AACAGTGCTAACTCAATTCCATACTCCTCCTCATCACCTGGTACCTATGTGGGACCCCCTGGTGGTGGC
  GGTCCTCCAGGAACACCCATTATGCCCAGTCCCGCAGATTCAACAAATTCCAGTGACAACATCTACACA
  ATGATTAATCCAGTGCCGCCTGGAGGCAGCCGGTCCAACTTCCCGATGGGTCCCGGCTCGGACGGTCCG
  ATGGGCGGCATGGGTGGCATGGAGCCACACCACATGAATGGATCATTAGGGTCAGGCGACATAGACGGA
  CTTCCAAAAAATTCTCCTAACAACATAAGTGGCATTAGCAATCCTCCAGGCACCCCTCGAGATGACGGC
  GAGCTAGGAGGGAACTTCCTCCACTCCTTTCAGAACGACAATTATTCTCCAAGCATGACGATGAGTGTG
  TGA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_018070
• Insert Size: 1107 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018070.4
• RefSeq Size: 3199 bp
• RefSeq ORF: 1107 bp
• Locus ID: 23648
• UniProt ID: Q9BWW4
• Cytogenetics: 1p32.3
• Domains: LisH, SSDP
• Protein Families: Transcription Factors
• MW: 38.2 kDa
• Gene Summary: May be involved in transcription regulation of the alpha 2(I) collagen gene where it binds to the single-stranded polypyrimidine sequences in the promoter region.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks an alternate in-frame exon, compared to variant 1. The encoded isoform (b) is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.