Rho GTPase activating protein 24 (ARHGAP24) (NM_031305) Human Untagged Clone

CAT#: SC310191

ARHGAP24 (untagged)-Human Rho GTPase activating protein 24 (ARHGAP24), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Rho GTPase activating protein 24
• Synonyms: FILGAP; p73; p73RhoGAP; RC-GAP72; RCGAP72
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC310191 representing NM_031305.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGCCTGAAGACCGGAATTCTGGGGGGTGCCCGGCTGGTGCCTTAGCCTCAACTCCTTTCATCCCTAAA
  ACTACATACAGAAGAATCAAACGGTGTTTTAGTTTTCGGAAAGGCATTTTTGGACAGAAACTGGAGGAT
  ACTGTTCGTTATGAGAAGAGATATGGGAACCGTCTGGCTCCGATGTTGGTGGAGCAGTGCGTGGACTTT
  ATCCGACAAAGGGGGCTGAAAGAAGAGGGTCTCTTTCGACTGCCAGGCCAGGCTAATCTTGTTAAGGAG
  CTCCAAGATGCCTTTGACTGTGGGGAGAAGCCATCATTTGACAGCAACACAGATGTACACACGGTGGCA
  TCACTTCTTAAGCTGTACCTCCGAGAACTTCCAGAACCAGTTATTCCTTATGCGAAGTATGAAGATTTT
  TTGTCATGTGCCAAACTGCTCAGCAAGGAAGAGGAAGCAGGTGTTAAGGAATTAGCAAAGCAGGTGAAG
  AGTTTGCCAGTGGTAAATTACAACCTCCTCAAGTATATTTGCAGATTCTTGGATGAAGTACAGTCCTAC
  TCGGGAGTTAACAAAATGAGTGTGCAGAACTTGGCAACGGTCTTTGGTCCTAATATCCTGCGCCCCAAA
  GTGGAAGATCCTTTGACTATCATGGAGGGCACTGTGGTGGTCCAGCAGTTGATGTCAGTGATGATTAGC
  AAACATGATTGCCTCTTTCCCAAAGATGCAGAACTACAAAGCAAGCCCCAAGATGGAGTGAGCAACAAC
  AATGAAATTCAGAAGAAAGCCACCATGGGGCAGTTACAGAACAAGGAGAACAATAACACCAAGGACAGC
  CCTAGTAGGCAGTGCTCCTGGGACAAGTCTGAGTCACCCCAGAGAAGCAGCATGAACAATGGATCCCCC
  ACAGCTCTATCAGGCAGCAAAACCAACAGCCCAAAGAACAGTGTTCACAAGCTAGATGTGTCTAGAAGC
  CCCCCTCTCATGGTCAAAAAGAACCCAGCCTTTAATAAGGGTAGTGGGATAGTTACCAATGGGTCCTTC
  AGCAGCAGTAATGCAGAAGGTCTTGAGAAAACCCAAACCACCCCCAATGGGAGCCTACAGGCCAGAAGG
  AGCTCTTCACTGAAGGTATCTGGTACCAAAATGGGCACGCACAGTGTACAGAATGGAACGGTGCGCATG
  GGCATTTTGAACAGCGACACACTCGGGAACCCCACAAATGTTCGAAACATGAGCTGGCTGCCAAATGGC
  TATGTGACCCTGAGGGATAACAAGCAGAAAGAACAAGCTGGAGAGTTAGGCCAGCACAACAGACTGTCC
  ACCTATGATAATGTCCATCAACAGTTCTCCATGATGAACCTTGATGACAAGCAGAGCATTGACAGTGCT
  ACCTGGTCCACTTCCTCCTGTGAAATCTCCCTCCCTGAGAACTCCAACTCCTGTCGCTCTTCTACCACC
  ACCTGCCCAGAGCAAGACTTTTTTGGGGGGAACTTTGAGGACCCTGTTTTGGATGGGCCCCCGCAGGAC
  GACCTTTCCCACCCCAGGGACTATGAAAGCAAAAGTGACCACAGGAGTGTGGGAGGTCGAAGTAGTCGT
  GCCACCAGTAGCAGTGACAACAGTGAGACATTTGTGGGCAACAGCAGCAGCAACCACAGTGCACTGCAC
  AGTTTAGTTTCCAGCCTGAAACAGGAAATGACCAAACAGAAGATAGAGTATGAGTCCAGGATAAAGAGC
  TTAGAACAGCGAAACTTGACTTTGGAAACAGAAATGATGAGCCTCCATGATGAACTGGATCAGGAGAGG
  AAAAAGTTCACAATGATAGAAATAAAAATGCGAAATGCCGAGCGAGCAAAAGAAGATGCCGAGAAAAGA
  AATGACATGCTACAGAAAGAAATGGAGCAGTTTTTTTCCACGTTTGGAGAACTGACAGTGGAACCCAGG
  AGAACCGAGAGAGGAAACACAATATGGATTCAGTGA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_031305
• Insert Size: 1968 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_031305.2
• RefSeq Size: 4574 bp
• RefSeq ORF: 1968 bp
• Locus ID: 83478
• UniProt ID: Q8N264
• Cytogenetics: 4q21.23-q21.3
• Domains: RhoGAP
• MW: 73.4 kDa
• Gene Summary: This gene encodes a Rho-GTPase activating protein, which is specific for the small GTPase family member Rac. Binding of the encoded protein by filamin A targets it to sites of membrane protrusion, where it antognizes Rac. This results in suppression of lamellae formation and promotion of retraction to regulate cell polarity. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2016]
  Transcript Variant: This variant (2) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (2) has a shorter and distinct N-terminus compared to isoform 1.