PACE4 (PCSK6) (NM_138320) Human Untagged Clone

CAT#: SC309533

PCSK6 (untagged)-Human proprotein convertase subtilisin/kexin type 6 (PCSK6), transcript variant 7

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PACE4
• Synonyms: PACE4; SPC4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC309533 representing NM_138320.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGCCTCCGCGCGCGCCGCCTGCGCCCGGGCCCCGGCCGCCGCCCCGGGCCGCCGCCGCCACCGACACC
  GCCGCGGGCGCGGGGGGCGCGGGGGGCGCGGGGGGCGCCGGCGGGCCCGGGTTCCGGCCGCTCGCGCCG
  CGTCCCTGGCGCTGGCTGCTGCTGCTGGCGCTGCCTGCCGCCTGCTCCGCGCCCCCGCCGCGCCCCGTC
  TACACCAACCACTGGGCGGTGCAAGTGCTGGGCGGCCCGGCCGAGGCGGACCGCGTGGCGGCGGCGCAC
  GGGTACCTCAACTTGGGCCAGATTGGAAACCTGGAAGATTACTACCATTTTTATCACAGCAAAACCTTT
  AAAAGATCAACCTTGAGTAGCAGAGGCCCTCACACCTTCCTCAGAATGGACCCCCAGGTGAAATGGCTC
  CAGCAACAGGAAGTGAAACGAAGGGTGAAGAGACAGGTGCGAAGTGACCCGCAGGCCCTTTACTTCAAC
  GACCCCATTTGGTCCAACATGTGGTACCTGCATTGTGGCGACAAGAACAGTCGCTGCCGGTCGGAAATG
  AATGTCCAGGCAGCGTGGAAGAGGGGCTACACAGGAAAAAACGTGGTGGTCACCATCCTTGATGATGGC
  ATAGAGAGAAATCACCCTGACCTGGCCCCAAATTATGATTCCTACGCCAGCTACGACGTGAACGGCAAT
  GATTATGACCCATCTCCACGATATGATGCCAGCAATGAAAATAAACACGGCACTCGTTGTGCGGGAGAA
  GTTGCTGCTTCAGCAAACAATTCCTACTGCATCGTGGGCATAGCGTACAATGCCAAAATAGGAGGCATC
  CGCATGCTGGACGGCGATGTCACAGATGTGGTCGAGGCAAAGTCGCTGGGCATCAGACCCAACTACATC
  GACATTTACAGTGCCAGCTGGGGGCCGGACGACGACGGCAAGACGGTGGACGGGCCCGGCCGACTGGCT
  AAGCAGGCTTTCGAGTATGGCATTAAAAAGGGCCGGCAGGGCCTGGGCTCCATTTTCGTCTGGGCATCT
  GGGAATGGCGGGAGAGAGGGGGACTACTGCTCGTGCGATGGCTACACCAACAGCATCTACACCATCTCC
  GTCAGCAGCGCCACCGAGAATGGCTACAAGCCCTGGTACCTGGAAGAGTGTGCCTCCACCCTGGCCACC
  ACCTACAGCAGTGGGGCCTTTTATGAGCGAAAAATCGTCACCACGGATCTGCGTCAGCGCTGTACCGAT
  GGCCACACTGGGACCTCAGTCTCTGCCCCCATGGTGGCGGGCATCATCGCCTTGGCTCTAGAAGCAAAC
  AGCCAGTTAACCTGGAGGGACGTCCAGCACCTGCTAGTGAAGACATCCCGGCCGGCCCACCTGAAAGCG
  AGCGACTGGAAAGTGAACGGCGCGGGTCATAAAGTTAGCCATTTCTATGGATTTGGTTTGGTGGACGCA
  GAAGCTCTCGTTGTGGAGGCAAAGAAGTGGACAGCAGTGCCATCGCAGCACATGTGTGTGGCCGCCTCG
  GACAAGAGACCCAGGAGCATCCCCTTAGTGCAGGTGCTGCGGACTACGGCCCTGACCAGCGCCTGCGCG
  GAGCACTCGGACCAGCGGGTGGTCTACTTGGAGCACGTGGTGGTTCGCACCTCCATCTCACACCCACGC
  CGAGGAGACCTCCAGATCTACCTGGTTTCTCCCTCGGGAACCAAGTCTCAACTTCTGGCAAAGAGGTTG
  CTGGATCTTTCCAATGAAGGGTTTACAAACTGGGAATTCATGACTGTCCACTGCTGGGGAGAAAAGGCT
  GAAGGGCAGTGGACCTTGGAAATCCAAGATCTGCCATCCCAGGTCCGCAACCCGGAGAAGCAAGGGAAG
  TTGAAAGAATGGAGCCTCATACTGTATGGCACAGCAGAGCACCCGTACCACACCTTCAGTGCCCATCAG
  TCCCGCTCGCGGATGCTGGAGCTCTCAGCCCCAGAGCTGGAGCCACCCAAGGCTGCCCTGTCACCCTCC
  CAGGTGGAAGTTCCTGAAGATGAGGAAGATTACACAGCTCAATCCACCCCAGGCTCTGCTAATATTTTA
  CAGACCAGTGTGTGCCATCCGGAGTGTGGTGACAAAGGCTGTGATGGCCCCAATGCAGACCAGTGCTTG
  AACTGCGTCCACTTCAGCCTGGGGAGTGTCAAGACCAGCAGGAAGTGCGTGAGTGTGTGCCCCTTGGGC
  TACTTTGGGGACACAGCAGCAAGACGCTGTCGCCGGTGCCACAAGGGGTGTGAGACCTGCTCCAGCAGA
  GCTGCGACGCAGTGCCTGTCTTGCCGCCGCGGGTTCTATCACCACCAGGAGATGAACACCTGTGTGACC
  CTCTGTCCTGCAGGATTTTATGCTGATGAAAGTCAGAAAAATTGCCTTAAATGCCACCCAAGCTGTAAA
  AAGTGCGTGGATGAACCTGAGAAATGTACTGTCTGTAAAGAAGGATTCAGCCTTGCACGGGGCAGCTGC
  ATTCCTGACTGTGAGCCAGGCACCTACTTTGACTCAGAGCTGATCAGATGTGGGGAATGCCATCACACC
  TGCGGAACCTGCGTGGGGCCAGGCAGAGAAGAGTGCATTCACTGTGCGAAAAACTTCCACTTCCACGAC
  TGGAAGTGTGTGCCAGCCTGTGGTGAGGGCTTCTACCCAGAAGAGATGCCGGGCTTGCCCCACAAAGTG
  TGTCGAAGGTACGGTCCTCCTGGCGGGGAACGGCAGGCAGCTGTGTCCAGCAAAGGAGTGCCTGGAGGG
  CAGAGTCTGGCAGCATCTTCCCCAGGGGCCGGGGAGGGCATGTTGCATCACCCCACTGTGGACAGGAGC
  CCATTTACAGAGCTGCTGAGGGGCCTTCGTCCCTTTGTTCACTGGATGCATATTTGTTGGGTCCCTGCT
  GTGGGCAGACACCGTGCAGCAGCGGGATAA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_138320
• Insert Size: 2928 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_138320.1
• RefSeq Size: 3372 bp
• RefSeq ORF: 2928 bp
• Locus ID: 5046
• Cytogenetics: 15q26.3
• Protein Families: Druggable Genome, Protease, Secreted Protein
• MW: 106.6 kDa
• Gene Summary: This gene encodes a member of the subtilisin-like proprotein convertase family, which includes proteases that process protein and peptide precursors trafficking through regulated or constitutive branches of the secretory pathway. The encoded protein undergoes an initial autocatalytic processing event in the ER to generate a heterodimer which exits the ER and sorts to the trans-Golgi network where a second autocatalytic event takes place and the catalytic activity is acquired. The encoded protease is constitutively secreted into the extracellular matrix and expressed in many tissues, including neuroendocrine, liver, gut, and brain. This gene encodes one of the seven basic amino acid-specific members which cleave their substrates at single or paired basic residues. Some of its substrates include transforming growth factor beta related proteins, proalbumin, and von Willebrand factor. This gene is thought to play a role in tumor progression and left-right patterning. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Feb 2014]
  Transcript Variant: This variant (7) utilizes an alternate splice site and lacks two downstream exons in the 3' coding region, compared to variant 1. This results in a frameshift and the encoded isoform PACE4-EI has a distinct C-terminus compared to isoform PACE4-AI. Isoform PACE4-EI is the longest isoform. The preproprotein is annotated since the cleavage sites are maintained as in isoform PACE4-AI.