WWOX (NM_130790) Human Untagged Clone

CAT#: SC309099

WWOX (untagged)-Human WW domain containing oxidoreductase (WWOX), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: WWOX
• Synonyms: FOR, WOX1, FRA16D, HHCMA56, PRO0128, WWOX v8, D16S432E
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_130790 edited
  GAGTGAGTTCCTGAGCGAGTGGACCCGGCAGCGGGCGATAGGGGGGCCAGGTGCCTCCAC
  AGTCAGCCATGGCAGCGCTGCGCTACGCGGGGCTGGACGACACGGACAGTGAGGACGAGC
  TGCCTCCGGGCTGGGAGGAGAGAACCACCAAGGACGGCTGGGTTTACTACGCCAATCACA
  CCGAGGAGAAGACTCAGTGGGAACATCCAAAAACTGGAAAAAGAAAACGAGTGGCAGGAG
  ATTTGCCATACGGATGGGAACAAGAAACTGATGAGAACGGACAAGTGTTTTTTGTTGACC
  ATATAAATAAAAGAACCACCTACTTGGACCCAAGACTGGCGTTTACTGTGGATGATAATC
  CGACCAAGCCAACCACCCGGCAAAGATACGACGGCAGCACCACTGCCATGGAAATTCTCC
  AGGGCCGGGATTTCACTGGCAAAGTGGTTGTGGTCACTGGAGCTAATTCAGGAATAGCAA
  CAGGGAGCTGCCACCACCGTGTACTGTGCTGCTGTCCCAGAACTGGAGGGTCTGGGAGGG
  ATGTACTTCAACAACTGCTGCCGCTGCATGCCCTCACCAGAAGCTCAGAGCGAAGAGACG
  GCCCGGACCCTGTGGGCGCTCAGCGAGAGGCTGATCCAAGAACGGCTTGGCAGCCAGTCC
  GGCTAAGTGGAGCTCAGAGCGGATGGGCACACACACCCGCCCTGTGTGTGTCCCCTCACG
  CAAGTGCCAGGGCTGGGCCCCTTCCAAATGTCCCTCCAACACAGATCCGCAAGAGTAAAG
  GAAATAAGAGCATTCACAACAGAGTGAAAAATCTTAAGTACCAATGGGAAGCAGGGAATT
  CCTGGGGTAAAGTATCACTTTTCTGGGGCTGGGCTAGGCATAGGTCTCTTTGCTTTCTGG
  TGGTGGCCTGTTTGAAAGTAAAAACCTGGTTGGCGTGTAGGTTCCGTATCTCCCTGGAAA
  AGCACCAGCAATTCTCTTTCTTTTACTGTTATAGAATAGCCTGAGGTCCCCTC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_130790 unedited
  GTCAAAATTTGTATACGACTCATATAGGCGGCCGCGNAATCAAATCTGGTACCGAGCTCG
  GATCCACTAGTAACGGCCGCCAGTGTGCTGGAATTCGCCCTTGAGTGAGTTCCTGAGCGA
  GTGGACCCGGCAGCGGGCGATAGGGGGGCCAGGTGCCTCCACAGTCAGCCATGGCAGCGC
  TGCGCTACGCGGGGCTGGACGACACGGACAGTGAGGACGAGCTGCCTCCGGGCTGGGAGG
  AGAGAACCACCAAGGACGGCTGGGTTTACTACGCCAATCACACCGAGGAGAAGACTCAGT
  GGGAACATCCAAAAACTGGAAAAAGAAAACGAGTGGCAGGAGATTTGCCATACGGATGGG
  AACAAGAAACTGATGAGAACGGACAAGTGTTTTTTGTTGACCATATAAATAAAAGAACCA
  CCTACTTGGACCCAAGACTGGCGTTTACTGTGGATGATAATCCGACCAAGCCAACCACCC
  GGCAAAGATACGACGGCAGCACCACTGCCATGGAAATTCTCCAGGGCCGGGATTTCACTG
  GCAAAGTGGTTGTGGTCACTGGAGCTAATTCAGGAATAGCAACAGGGAGCTGCCACCACC
  GTGTACTGTGCTGCTGTCCCAGAACTGGAGGGTCTGGGAGGGATGTACTTCAACAACTGC
  TGCCGCTGCATGCCCTCACCAGAAGCTCAGAGCGAAGAGACGGCCCGGACCCTGTGGGCG
  CTCAGCGAGAGGCTGATCCAAGAACGGCTTGGCAGCCAGTCCGGCTAAGTGGAGCTCAGA
  GCGGATGGGCACACACACCCGCCCTGTGTGTGTCCCCTCACGCAAGTGCCN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_130790 unedited
  CCATGGNGATGGCACTTCCAGGNCCAGGNANAGCACTGGGGNAGGGTCACAGGGATGCCA
  CCCGGGATCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGATGCATGCTCGAGC
  GGCCGCCAGTGTGATGGATATCTGCAGAATTCGCCCTTCGAGGGGACCTCAGGCTATTCT
  ATAACAGTAAAAGAAAGAGAATTGCTGGTGCTTTTCCAGGGAGATACGGAACCTACACGC
  CAACCAGGTTTTTACTTTCAAACAGGCCACCACCAGAAAGCAAAGAGACCTATGCCTAGC
  CCAGCCCCAGAAAAGTGATACTTTACCCCAGGAATTCCCTGCTTCCCATTGGTACTTAAG
  ATTTTTCACTCTGTTGTGAATGCTCTTATTTCCTTTACTCTTGCGGATCTGTGTTGGAGG
  GACATTTGGAAGGGGCCCAGCCCTGGCACTTGCGTGAGGGGACACACACAGGGCGGGTGT
  GTGTGCCCATCCGCTCTGAGCTCCACTTAGCCGGACTGGCTGCCAAGCCGTTCTTGGATC
  AGCCTCTCGCTGAGCGCCCACAGGGTCCGGGCCGTCTCTTCGCTCTGAGCTTCTGGTGAG
  GGCATGCAGCGGCAGCAGTTGTTGAAGTACATCCCTCCCAGACCCTCCAGTTCTGGGACA
  GCAGCACAGTACACGGTGGTGGCAGCTCCCTGTTGCTATTCCTGAATTAGCTCCAGTGAC
  CACAACCACTTTGCCAGTGAAATCCCGGCCCTGGANAAATTCCATGGCAGTGGTGCTGCC
  GTCGTATCTTTGCCCGGTGGTTGGGCTTGGTCGGATTATCATCCACCAGTAACGCCCAGT
  CTTGGGTCCCAAGTAGTGGGTTCTTTTATTTATATGGGTCAC
  
• Restriction Sites: Please inquire
• ACCN: NM_130790
• Insert Size: 1000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_130790.1, NP_570606.1
• RefSeq Size: 1617 bp
• RefSeq ORF: 1617 bp
• Locus ID: 51741
• Cytogenetics: 16q23.1-q23.2
• Protein Families: Druggable Genome
• Gene Summary: This gene encodes a member of the short-chain dehydrogenases/reductases (SDR) protein family. This gene spans the FRA16D common chromosomal fragile site and appears to function as a tumor suppressor gene. Expression of the encoded protein is able to induce apoptosis, while defects in this gene are associated with multiple types of cancer. Disruption of this gene is also associated with autosomal recessive spinocerebellar ataxia 12. Disruption of a similar gene in mouse results in impaired steroidogenesis, additionally suggesting a metabolic function for the protein. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]
  Transcript Variant: This variant (3) lacks a 647 nt fragment in the middle part of the coding region, as compared to variant 1. The absence of the internal fragment shifts the reading frame and results in a smaller isoform (3) which lacks the SRD region and has a different C-terminus from that of isoform 1.