DYRK1B (NM_006484) Human Untagged Clone

CAT#: SC308963

DYRK1B (untagged)-Human dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1B (DYRK1B), transcript variant c

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DYRK1B
• Synonyms: AOMS3; MIRK
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC308963 representing NM_006484.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGCCGTCCCACCGGGCCATGGTCCCTTCTCTGGCTTCCCAGGGCCCCAGGAGCACACGCAGGTATTG
  CCTGATGTGCGGCTACTGCCTCGGAGGCTGCCCCTGGCCTTCCGGGATGCAACCTCAGCCCCGCTGCGT
  AAGCTCTCTGTGGACCTCATCAAGACCTACAAGCACATCAATGAGGTATACTATGCGAAGAAGAAGCGG
  CGGGCCCAGCAGGCGCCACCCCAGGATTCGAGCAACAAGAAGGAGAAGAAGGTCCTGAACCATGGTTAT
  GATGACGACAACCATGACTACATCGTGCGCAGTGGCGAGCGCTGGCTGGAGCGCTACGAAATTGACTCG
  CTCATTGGCAAAGGCTCCTTTGGCCAGGTGGTGAAAGCCTATGATCATCAGACCCAGGAGCTTGTGGCC
  ATCAAGATCATCAAGAACAAAAAGGCTTTCCTGAACCAGGCCCAGATTGAGCTGCGGCTGCTGGAGCTG
  ATGAACCAGCATGACACGGAGATGAAGTACTATATAGTACACCTGAAGCGGCACTTCATGTTCCGGAAC
  CACCTGTGCCTGGTATTTGAGCTGCTGTCCTACAACCTGTACGACCTCCTGCGCAACACCCACTTCCGC
  GGCGTCTCGCTGAACCTGACCCGGAAGCTGGCGCAGCAGCTCTGCACGGCACTGCTCTTTCTGGCCACG
  CCTGAGCTCAGCATCATTCACTGCGACCTCAAGCCCGAAAACATCTTGCTGTGCAACCCCAAGCGCAGC
  GCCATCAAGATTGTGGACTTCGGCAGCTCCTGCCAGCTTGGCCAGAGGATCTACCAGTATATCCAGAGC
  CGCTTCTACCGCTCACCTGAGGTGCTCCTGGGCACACCCTACGACCTGGCCATTGACATGTGGTCCCTG
  GGCTGCATCCTTGTGGAGATGCACACCGGAGAGCCCCTCTTCAGTGGCTCCAATGAGGTCGACCAGATG
  AACCGCATTGTGGAGGTGCTGGGCATCCCACCGGCCGCCATGCTGGACCAGGCGCCCAAGGCTCGCAAG
  TACTTTGAACGGCTGCCTGGGGGTGGCTGGACCCTACGAAGGACGAAAGAACTCAGGAAGGATTACCAG
  GGCCCCGGGACACGGCGGCTGCAGGAGGACCTGGTGCTGCGCATGCTGGAGTATGAGCCCGCCGCCCGC
  ATCAGCCCCCTGGGGGCTCTGCAGCACGGCTTCTTCCGCCGCACGGCCGACGAGGCCACCAACACGGGC
  CCGGCAGGCAGCAGTGCCTCCACCTCGCCCGCGCCCCTCGACACCTGCCCCTCTTCCAGCACCGCCAGC
  TCCATCTCCAGTTCTGGAGGCTCCAGTGGCTCCTCCAGTGACAACCGGACCTACCGCTACAGCAACCGA
  TATTGTGGGGGCCCTGGGCCCCCTATCACAGACTGTGAGATGAACAGCCCCCAGGTCCCACCCTCCCAG
  CCGCTGCGGCCCTGGGCAGGGGGTGATGTGCCCCACAAGACACATCAAGCCCCTGCCTCTGCCTCGTCA
  CTGCCTGGGACCGGGGCCCAGTTACCCCCCCAGCCCCGATACCTTGGTCGTCCCCCATCACCAACCTCA
  CCACCACCCCCGGAGCTGATGGATGTGAGCCTGGTGGGCGGCCCTGCTGACTGCTCCCCACCTCACCCA
  GCGCCTGCCCCCCAGCACCCGGCTGCCTCAGCCCTCCGGACTCGGATGACTGGAGGTCGTCCACCCCTC
  CCGCCTCCTGATGACCCTGCCACTCTGGGGCCTCACCTGGGCCTCCGTGGTGTACCCCAGAGCACAGCA
  GCCAGCTCGTGA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_006484
• Insert Size: 1806 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006484.2
• RefSeq Size: 2504 bp
• RefSeq ORF: 1806 bp
• Locus ID: 9149
• UniProt ID: Q9Y463
• Cytogenetics: 19q13.2
• Protein Families: Druggable Genome, Protein Kinase, Transcription Factors
• MW: 66.3 kDa
• Gene Summary: This gene encodes a member of a family of nuclear-localized protein kinases. The encoded protein participates in the regulation of the cell cycle. Expression of this gene may be altered in tumor cells, and mutations in this gene were found to cause abdominal obesity-metabolic syndrome 3. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2014]
  Transcript Variant: This variant (3, also known as 'c') lacks an alternate in-frame segment in the 3' coding region, compared to variant 1. The encoded isoform (p66, also known as isoform c) is shorter than isoform p69.