PCDHA8 (NM_031856) Human Untagged Clone

CAT#: SC308765

PCDHA8 (untagged)-Human protocadherin alpha 8 (PCDHA8), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PCDHA8
• Synonyms: PCDH-ALPHA8
• Vector: pCMV6 series
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_031856, the custom clone sequence may differ by one or more nucleotides
  ATGGATTATCACTGGCGAGGAGAGCTGGGATCCTGGCGACTACTACTCTTGCTTCTGCTC
  CTCGCAGCCTGGAAGGTGGGGAGCGGCCAGCTCCACTACTCCGTCCCCGAGGAGGCCAAA
  CACGGCACCTTCGTGGGCCGGATCGCGCAGGACCTGGGGCTGGAGCTGGCGGAGCTGGTG
  CCGCGCCTGTTCCGGGTGGCGTCCAAAAGACACCGGGACCTTCTGGAGGTAAGTCTGCAG
  AATGGCATTTTGTTTGTGAATTCTCGGATCGACCGCGAGGAGCTGTGCGGGCGGAGCGCG
  GAGTGCAGCATCCACCTGGAGGTGATCGTGGACAGGCCGCTGCAGGTTTTCCATGTGGAC
  GTGGAGGTGAAGGATGTTAATGACAACCCGCCAGTGTTCCGGGTAAAAGACCAAAAGCTG
  TTTGTTTCAGAATCCAGAATGCCAGACTCTCGGTTTCCGCTAGAGGGCGCGTCCGATGCA
  GATGTTGGAGCTAACTCCGTGTTAACCTACAGGCTTAGCTCTCATGATTACTTCATGCTA
  GATGTGAATTCAAAGAACGATGAGAATAAACTGGTTGAGCTCGTATTAAGAAAATCCTTG
  GACAGAGAGGACGCTCCTGCGCACCACTTATTCCTGACAGCCACAGATGGGGGCAAACCT
  GAGCTCACAGGCACTGTTCAGCTGCTGGTCACAGTGCTGGATGTGAATGATAATGCTCCC
  ACTTTCGAACAGTCTGAATACGAAGTAAGAATATTCGAAAACGCAGACAACGGAACAACA
  GTTATCAAACTGAATGCTTCTGATCCGGATGAAGGAGCCAATGGGGCAATTTCATATTCT
  TTTAATAGCCTTGTTGAAACTATGGTTATTGACCACTTTAGCATAGATCGAAATACGGGA
  GAAATAGTGATTCGGGGTAATTTGGATTTTGAACAAGAAAACTTATACAAAATCCTCATT
  GACGCCACGGACAAAGGCCATCCTCCCATGGCGGGTCATTGCACCGTTTTAGTGAGAATT
  TTGGATAAAAATGATAACGTCCCTGAGATAGCACTGACTTCCTTATCCTTGCCTGTACGT
  GAAGACGCTCAATTTGGTACTGTCATCGCCCTAATTAGCGTGAACGACCTCGATTCAGGT
  GCCAACGGGCAGGTGACCTGCTCCCTGATGCCCCATGTCCCCTTCAAGCTGGTGTCCACC
  TTCAAGAATTACTACTCGTTGGTGCTGGACAGCGCCCTGGACCGCGAGAGAGTGTCGGCC
  TATGAGTTGGTGGTAACCGCGCGGGACGGGGGCTCGCCTTCGCTGTGGGCCACCGCCAGC
  TTGTCTGTGGAGGTGGCCGACGTGAACGACAATGCTCCGGCGTTCGCGCAGCCCGAGTAC
  ACGGTGTTCGTGAAGGAGAACAACCCGCCGGGCTGCCACATCTTCACGGTGTCTGCGCGA
  GACGCGGACGCGCAGGAGAACGCGCTGGTGTCCTACTCGCTTGTGGAGCGGCGGGTGGGC
  GAGCGCTCGCTGTCGAGCTACATTTCGGTGCACACGGAGAGCGGCAAGGTGTACGCGCTG
  CAGCCGCTGGACCACGAGGAGCTAGAGCTGCTGCAGTTCCAGGTGAGCGCGCGCGACGCG
  GGCGTGCCGCCTCTGGGCAGCAACGTGACGCTGCAGGTGTTCGTGCTGGACGAGAATGAC
  AACGCGCCGGCACTGCTGGAGCCTCGGGTGGGTGGCACTGGTGGCGCAGCGAGCAAGCTG
  GTGCCGCGGTCTGTGGGCGCGGGCCACGTGGTAGCGAAGGTGCGCGCAGTGGACGCCGAC
  TCGGGCTACAACGCGTGGCTTTCGTATGAGCTGCAGCCAGCTGCAAGCAGCCCTCGCATC
  CCGTTCCGCGTGGGGCTGTACACGGGCGAGATCAGCACCACTCGTGTCCTGGACGAAGCG
  GACTCTCCGCGCCACCGTCTGCTGGTCCTGGTGAAGGATCATGGTGAACCTGCGCTGACC
  GCCACGGCCACGGTTCTGGTGTCGCTGGTGGAGAGCGGCCAGGCTCCAAAAGCGTCATCG
  AGGCAGTCGGCTGGCGTTTTGGGTCCGGAAGCGGCGCTGGTGGATGTCAACGTGTACCTG
  ATCATCGCCATCTGCGCGGTATCCAGCCTGCTGGTGCTCACGCTGCTGCTGTACACTGCG
  CTGCGGTGCTCAGCACTGCCCACTGAGGGCGGGTGCCGGGCGGGCAAGCCCACTCTGGTG
  TGCTCCAGTGCGGTGGGGAGCTGGTCATACTCGCAACAACAGCCGCAGAGGGTGTGCTCT
  GGTGAGGGGCCACCGAAGACGGACCTCATGGCCTTCAGCCCCTGCCTTCCTCCTGATCTG
  GGATCAGTTGATGTAGGCGAAGAGCAAGATTTAAATGTTGATCATGGCCTCAAAGTAAGT
  CCATTTAAATTTAGAACTCATAAATTCTATTTGTGGAAATTGTAG
  
• Restriction Sites: Please inquire
• ACCN: NM_031856
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_031856.1, NP_114062.1
• RefSeq Size: 2445 bp
• RefSeq ORF: 2445 bp
• Locus ID: 56140
• UniProt ID: Q9Y5H6
• Cytogenetics: 5q31.3
• Protein Families: Transmembrane
• Gene Summary: This gene is a member of the protocadherin alpha gene cluster, one of three related gene clusters tandemly linked on chromosome five that demonstrate an unusual genomic organization similar to that of B-cell and T-cell receptor gene clusters. The alpha gene cluster is composed of 15 cadherin superfamily genes related to the mouse CNR genes and consists of 13 highly similar and 2 more distantly related coding sequences. The tandem array of 15 N-terminal exons, or variable exons, are followed by downstream C-terminal exons, or constant exons, which are shared by all genes in the cluster. The large, uninterrupted N-terminal exons each encode six cadherin ectodomains while the C-terminal exons encode the cytoplasmic domain. These neural cadherin-like cell adhesion proteins are integral plasma membrane proteins that most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. Alternative splicing has been observed and additional variants have been suggested but their full-length nature has yet to be determined. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) utilizes the large, first exon then continues into the downstream intron 1 sequence before terminating. This one-exon transcript encodes a shorter isoform (2), compared to isoform 1. This short variant is represented based on data in PMID:10380929.