CAMSAP1L1 (CAMSAP2) (NM_203459) Human Untagged Clone

CAT#: SC308193

CAMSAP2 (untagged)-Human calmodulin regulated spectrin-associated protein 1-like 1 (CAMSAP1L1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CAMSAP1L1
• Synonyms: CAMSAP1L1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC308193 representing NM_203459.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGGGGATGCTGCAGACCCCAGGGAGATGAGAAAGACGTTCATTGTTCCAGCCATCAAGCCTTTTGAC
  CACTATGATTTCTCCAGGGCCAAAATCGCCTGCAATCTGGCCTGGCTGGTGGCCAAAGCCTTTGGGACA
  GAAAATGTGCCAGAGGAACTTCAAGAACCATTTTACACAGATCAGTATGACCAGGAACACATCAAACCA
  CCTGTTGTTAATTTGCTTCTATCGGCTGAACTATACTGTCGTGCTGGGAGTCTCATTCTCAAGAGTGAT
  GCTGCAAAACCCCTTTTGGGCCATGATGCTGTAATCCAGGCTTTAGCACAGAAAGGTCTTTATGTCACT
  GACCAGGAAAAATTGGTAACTGAACGAGATCTCCACAAGAAACCCATACAGATGAGTGCACATTTGGCC
  ATGATCGATACCCTCATGATGGCTTATACTGTAGAAATGGTCAGTATAGAAAAAGTAATTGCGTGTGCT
  CAGCAGTATTCAGCTTTTTTTCAAGCCACAGATCTGCCCTATGATATTGAGGACGCTGTCATGTACTGG
  ATAAATAAGGTAAATGAACATTTGAAAGACATAATGGAACAAGAACAAAAACTGAAAGAACATCACACA
  GTTGAAGCTCCAGGAGGTCAAAAGGCTCGTTATCGGAAAGAGCAAACATTGCTTAAGCAACTGCCTTGC
  ATTCCATTGGTAGAAAATTTGTTGAAGGATGGGACAGATGGCTGTGCATTAGCTGCCCTTATTCATTTT
  TACTGTCCTGATGTTGTCAGATTAGAGGATATTTGTTTGAAAGAAACTATGTCTTTGGCTGATAGCCTG
  TATAATCTGCAGCTGATTCAAGAATTTTGCCAAGAATACTTGAACCAGTGTTGCCATTTCACTCTGGAA
  GATATGCTCTATGCTGCTTCATCCATAAAGAGTAATTATTTGGTGTTCATGGCGGAACTGTTCTGGTGG
  TTTGAAGTGGTGAAGCCGTCTTTTGTACAGCCTCGTGTTGTTCGTCCACAAGGAGCTGAACCTGTAAAA
  GATATGCCTTCAATTCCTGTCTTGAATGCTGCCAAAAGAAATGTCTTAGATAGTAGTTCTGACTTCCCT
  TCAAGTGGGGAAGGAGCTACATTTACACAGTCTCATCATCATTTGCCTTCTAGGTATTCACGTCCCCAG
  GCTCATTCTTCAGCCTCAGGAGGAATTAGAAGGTCTTCATCTATGTCTTATGTTGATGGCTTCATAGGG
  ACATGGCCCAAAGAGAAAAGATCATCAGTGCATGGCGTATCATTTGATATTTCTTTTGATAAAGAAGAT
  AGTGTACAGAGATCCACTCCAAACCGAGGAATCACTCGTTCTATTAGTAATGAAGGACTTACTCTGAAC
  AACAGTCATGTATCTAAACACATTAGGAAAAATTTGTCCTTCAAGCCAATAAATGGAGAAGAGGAAGCA
  GAGAGCATTGAAGAAGAACTTAATATAGATTCTCACAGTGACCTCAAATCTTGTGTGCCCCTTAACACA
  AATGAACTAAATTCTAATGAGAATATTCATTACAAGCTTCCAAATGGAGCTTTACAAAATAGAATACTT
  CTTGACGAGTTTGGCAATCAGATCGAGACACCAAGCATTGAAGAAGCATTACAAATAATTCATGATACT
  GAAAAATCTCCTCATACACCTCAGCCAGACCAAATTGCTAATGGCTTCTTTCTTCATAGTCAAGAAATG
  AGTATCTTAAATTCAAATATCAAGTTAAATCAATCTAGTCCTGATAATGTAACTGATACGAAAGGTGCC
  TTGAGTCCCATAACTGACAATACTGAAGTAGACACTGGAATTCACGTTCCTTCAGAAGATATTCCTGAA
  ACTATGGACGAAGATTCTTCGTTGAGAGATTATACTGTAAGCTTGGACTCTGACATGGATGATGCATCT
  AAATTTCTTCAGGATTATGATATTCGAACTGGCAACACCAGGGAAGCTTTGAGTCCTTGTCCAAGTACT
  GTAAGTACCAAGTCTCAGCCAGGCAGCAGTGCTTCTTCTAGTTCTGGAGTTAAAATGACCAGCTTTGCT
  GAACAAAAATTCAGGAAACTGAATCATACCGATGGAAAAAGTAGTGGAAGCAGTTCTCAAAAAACTACA
  CCAGAAGGCTCTGAACTTAATATTCCTCATGTGGTTGCTTGGGCACAAATTCCAGAAGAAACAGGGCTT
  CCACAGGGACGGGACACTACCCAGCTGTTGGCCTCTGAAATGGTGCATCTTAGGATGAAACTAGAAGAA
  AAGAGGCGTGCTATAGAAGCCCAGAAAAAGAAAATGGAAGCTGCTTTTACCAAACAGAGACAGAAAATG
  GGAAGGACAGCATTCCTTACTGTAGTGAAAAAGAAAGGGGATGGGATATCTCCTCTACGAGAGGAAGCG
  GCGGGTGCAGAAGATGAGAAAGTATATACTGATCGAGCAAAAGAAAAGGAATCACAAAAAACTGATGGA
  CAAAGGAGCAAGTCACTGGCAGATATAAAAGAGAGCATGGAGAATCCTCAAGCCAAATGGCTAAAGTCT
  CCAACTACACCTATTGATCCTGAGAAGCAGTGGAACCTGGCAAGCCCCTCAGAAGAAACTTTAAATGAA
  GGAGAGATTTTAGAATATACCAAATCCATTGAAAAGTTAAATTCATCCCTGCATTTTCTACAACAAGAA
  ATGCAACGCTTGTCACTTCAGCAGGAGATGTTAATGCAGATGAGAGAGCAACAATCTTGGGTGATTTCA
  CCTCCACAACCCTCTCCACAGAAACAGATTCGAGATTTTAAGCCTTCTAAGCAGGCAGGCCTGTCATCA
  GCCATTGCACCATTCTCCTCAGACTCCCCTCGTCCTACTCACCCATCTCCACAGTCTTCTAACAGGAAA
  AGTGCATCTTTTTCTGTTAAAAGTCAAAGGACTCCTAGGCCAAATGAGTTAAAAATAACACCTTTGAAT
  CGAACCTTGACACCTCCTCGGTCTGTGGATAGCCTTCCTCGGTTAAGGAGGTTTTCACCAAGTCAAGTT
  CCTATTCAAACTAGGTCATTTGTATGTTTTGGGGATGATGGAGAACCTCAGTTAAAGGAATCCAAACCT
  AAAGAGGAAGTTAAAAAGGAGGAATTGGAATCCAAAGGGACTTTGGAACAGCGTGGACATAATCCAGAA
  GAAAAGGAAATCAAACCTTTTGAGTCAACAGTCTCTGAAGTCCTATCACTGCCTGTCACAGAGACTGTA
  TGTCTGACACCAAATGAGGACCAATTGAATCAACCCACAGAACCCCCTCCTAAACCCGTTTTCCCACCC
  ACTGCTCCAAAAAATGTTAATCTGATTGAAGTTTCCCTCTCAGATTTGAAACCCCCTGAAAAGGCTGAT
  GTACCTGTTGAAAAATATGATGGAGAAAGTGATAAAGAACAATTTGATGATGACCAGAAAGTATGCTGT
  GGATTCTTTTTTAAGGATGATCAAAAAGCAGAAAATGATATGGCAATGAAACGGGCAGCTTTGTTGGAG
  AAAAGATTAAGAAGGGAAAAGGAAACTCAGCTCCGGAAACAACAGTTGGAAGCAGAAATGGAGCATAAG
  AAGGAGGAAACAAGGCGTAAAACTGAGGAAGAACGTCAGAAGAAAGAAGATGAGAGAGCACGCAGAGAA
  TTTATTAGGCAAGAATATATGAGGCGGAAACAACTGAAACTAATGGAAGATATGGATACAGTAATTAAA
  CCCCGTCCTCAAGTAGTAAAACAAAAAAAACAGCGACCAAAATCTATTCACAGAGATCATATTGAATCC
  CCCAAAACACCAATAAAGGGTCCTCCAGTCTCTAGCCTTTCTTTGGCATCGCTGAACACGGGTGATAAC
  GAGAGTGTACATTCAGGCAAGAGGACGCCAAGATCAGAGTCTGTAGAAGGCTTCTTATCTCCAAGTCGT
  TGTGGCAGTCGAAATGGAGAAAAAGACTGGGAGAATGCATCAACAACTTCTTCAGTGGCTTCTGGAACA
  GAATATACAGGACCAAAGCTCTACAAAGAACCCAGTGCAAAATCCAATAAGCACATAATACAAAATGCT
  TTAGCTCATTGCTGTTTGGCTGGAAAAGTAAATGAAGGTCAGAAGAAAAAAATACTGGAGGAAATGGAG
  AAATCAGATGCCAACAACTTCTTAATCTTGTTCCGGGATTCAGGATGCCAGTTCAGATCTTTATACACT
  TATTGCCCAGAAACTGAAGAAATCAATAAACTGACTGGGATAGGCCCTAAATCTATCACTAAAAAAATG
  ATTGAAGGACTTTACAAATATAATTCTGACAGGAAACAGTTTAGCCACATACCCGCTAAAACTTTATCT
  GCCAGTGTTGATGCAATTACCATTCATAGCCATTTATGGCAGACCAAAAGACCAGTAACACCCAAAAAA
  CTTTTACCCACTAAGGCATAG
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_203459
• Insert Size: 4437 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_203459.2
• RefSeq Size: 7369 bp
• RefSeq ORF: 4437 bp
• Locus ID: 23271
• UniProt ID: Q08AD1
• Cytogenetics: 1q32.1
• MW: 166.7 kDa
• Gene Summary: Key microtubule-organizing protein that specifically binds the minus-end of non-centrosomal microtubules and regulates their dynamics and organization (PubMed:23169647, PubMed:24486153, PubMed:24706919). Specifically recognizes growing microtubule minus-ends and autonomously decorates and stabilizes microtubule lattice formed by microtubule minus-end polymerization (PubMed:24486153, PubMed:24706919). Acts on free microtubule minus-ends that are not capped by microtubule-nucleating proteins or other factors and protects microtubule minus-ends from depolymerization (PubMed:24486153, PubMed:24706919). In addition, it also reduces the velocity of microtubule polymerization (PubMed:24486153, PubMed:24706919). Through the microtubule cytoskeleton, also regulates the organization of cellular organelles including the Golgi and the early endosomes (PubMed:27666745). Essential for the tethering, but not for nucleation of non-centrosomal microtubules at the Golgi: together with Golgi-associated proteins AKAP9 and PDE4DIP, required to tether non-centrosomal minus-end microtubules to the Golgi, an important step for polarized cell movement (PubMed:27666745). Also acts as a regulator of neuronal polarity and development: localizes to non-centrosomal microtubule minus-ends in neurons and stabilizes non-centrosomal microtubules, which is required for neuronal polarity, axon specification and dendritic branch formation (PubMed:24908486). Through the microtubule cytoskeleton, regulates the autophagosome transport (PubMed:28726242).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks an alternate in-frame exon, compared to variant 1. The encoded isoform (2) is shorter than isoform 1.