5HT4 Receptor (HTR4) (NM_199453) Human Untagged Clone

CAT#: SC307982

HTR4 (untagged)-Human 5-hydroxytryptamine (serotonin) receptor 4 (HTR4), transcript variant g

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: 5HT4 Receptor
• Synonyms: 5-HT4; 5-HT4R
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_199453 edited
  CGCAGGCAGCGGCTGGGAGCGCAGCGGCAGCGGCGGCAGCAGAAGCTCGGCTCAGCGGCT
  GGGGGTGGCCGCTCGAATCTGCCAGGGCACCTCGCTCCCTCGCCTCTGGCAGCGGGACCC
  TGTGGGCATTGAAATCCAACTCACTCATGCTTATTTCCTGTAATGGACAAACTTGATGCT
  AATGTGAGTTCTGAGGAGGGTTTCGGGTCAGTGGAGAAGGTGGTGCTGCTCACGTTTCTC
  TCGACGGTTATCCTGATGGCCATCTTGGGGAACCTGCTGGTGATGGTGGCTGTGTGCTGG
  GACAGGCAGCTCAGGAAAATAAAAACAAATTATTTCATTGTATCTCTTGCTTTTGCGGAT
  CTGCTGGTTTCGGTGCTGGTGATGCCCTTTGGTGCCATTGAGCTGGTTCAAGACATCTGG
  ATTTATGGGGAGGTGTTTTGTCTTGTTCGGACATCTCTGGACGTCCTGCTCACAACGGCA
  TCGATTTTTCACCTGTGCTGCATTTCTCTGGATAGGTATTACGCCATCTGCTGCCAGCCT
  TTGGTCTATAGGAACAAGATGACCCCTCTGCGCATCGCATTAATGCTGGGAGGCTGCTGG
  GTCATCCCCACGTTTATTTCTTTTCTCCCTATAATGCAAGGCTGGAATAACATTGGCATA
  ATTGATTTGATAGAAAAGAGGAAGTTCAACCAGAACTCTAACTCTACGTACTGTGTCTTC
  ATGGTCAACAAGCCCTACGCCATCACCTGCTCTGTGGTGGCCTTCTACATCCCATTTCTC
  CTCATGGTGCTGGCCTATTACCGCATCTATGTCACAGCTAAGGAGCATGCCCATCAGATC
  CAGATGTTACAACGGGCAGGAGCCTCCTCCGAGAGCAGGCCTCAGTCGGCAGACCAGCAT
  AGCACTCATCGCATGAGGACAGAGACCAAAGCAGCCAAGACCCTGTGCATCATCATGGGT
  TGCTTCTGCCTCTGCTGGGCACCATTCTTTGTCACCAATATTGTGGATCCTTTCATAGAC
  TACACTGTCCCTGGGCAGGTGTGGACTGCTTTCCTCTGGCTCGGCTATATCAATTCCGGG
  TTGAACCCTTTTCTCTACGCCTTCTTGAATAAGTCTTTTAGACGTGCCTTCCTCATCATC
  CTCTGCTGTGATGATGAGCGCTACCGAAGACCTTCCATTCTGGGCCAGACTGTCCCTTGT
  TCAACCACAACCATTAATGGATCCACACATGTACTAAGTGGCTGTTCCCCTGTCTCCAGC
  TTCCTCCTGCTCTTCTGCAATAGACCAGTTCCTGTCTAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_199453 unedited
  GGCTACTTGTGTATACGACTCCTATAGGGCGGCCGCGAATTCGGCACGAGGCGCAGGCAG
  CGGCTGGGAGCGCAGCGGCAGCGGCGGCAGCAGAAGCTCGGCTCAGCGGCTGGGGGTGGC
  CGCTCGAATCTGCCAGGGCACCTCGCTCCCTCGCCTCTGGCAGCGGGACCCTGTGGGCAT
  TGAAATCCAACTCACTCATGCTTATTTCCTGTAATGGACAAACTTGATGCTAATGTGAGT
  TCTGAGGAGGGTTTCGGGTCAGTGGAGAAGGTGGTGCTGCTCACGTTTCTCTCGACGGTT
  ATCCTGATGGCCATCTTGGGGAACCTGCTGGTGATGGTGGCTGTGTGCTGGGACAGGCAG
  CTCAGGAAAATAAAAACAAATTATTTCATTGTATCTCTTGCTTTTGCGGATCTGCTGGTT
  TCGGTGCTGGTGATGCCCTTTGGTGCCATTGAGCTGGTTCAAGACATCTGGATTTATGGG
  GAGGTGTTTTGTCTTGTTCGGACATCTCTGGACGTCCTGCTCACAACGGCATCGATTTTT
  CACCTGTGCTGCATTTCTCTGGATAGGTATTACGCCATCTGCTGCCAGCCTTTGGTCTAT
  AGGAACAAGATGACCCCTCTGCGCATCGCATTAATGCTGGGAGGCTGCTGGGTCATCCCC
  ACGTTTATTTCTTTTCTCCCTATAATGCAAGGCTGGAATAACATTGGCATAATTGATTTG
  ATAGAAAAGAGGAAGTTCAACCAGAACTCTAACTCTACGTACTGTGTCTTCATGGTCAAC
  AAGCCCTACGCCATCACCTGCTCTGTGGTGGCCTTCTACATCCCATTTCTCCTCATGGTG
  CTGGCCTATTACCGCATCTATGTCACAGCTAAGGAGCATGCCCATCAGATCCAGATGTTA
  CAACGGGCAGGAGCCTCCTCCGAGAAGCAGGCCCTCAGTCGGCAGA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_199453 unedited
  CGTGATGCACTTCAGGGCCGGAGAGGCACTGGGGAGGGGTCACAGGGATGCCACCCGGGA
  TCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGATTAGACAGGAACTGGTCTAT
  TGCAGAAGAGCAGGAGGAAGCTGGAGACAGGGGAACAGCCACTTAGTACATGTGTGGATC
  CATTAATGGTTGTGGTTGAACAAGGGACAGTCTGGCCCAGAATGGAAGGTCTTCGGTAGC
  GCTCATCATCACAGCAGAGGATGATGAGGAAGGCACGTCTAAAAGACTTATTCAAGAAGG
  CGTAGAGAAAAGGGTTCAACCCGGAATTGATATAGCCGAGCCAGAGGAAAGCAGTCCACA
  CCTGCCCAGGGACAGTGTAGTCTATGAAAGGATCCACAATATTGGTGACAAAGAATGGTG
  CCCAGCAGAGGCAGAAGCAACCCATGATGATGCACAGGGTCTTGGCTGCTTTGGTCTCTG
  TCCTCATGCGATGAGTGCTATGCTGGTCTGCCGACTGAGGCCTGCTCTCGGAGGAGGCTC
  CTGCCCGTTGTAACATCTGGATCTGATGGGCATGCTCCTTAGCTGTGACATAGATGCGGT
  AATAGGCCAGCACCATGAGGAGAAATGGGATGTAGAAGGCCACCACAGAGCAGGTGATGG
  CGTAGGGCTTGTTGACCATGAAGACACAGTACGTAGAGTTAGAGTTCTGGTTGAACTTCC
  TCTTTTCTATCAAATCAATTATGCCAATGTTATTCCAGCCTTGCATTATAGGGAGAAAAG
  AAATAAACGTGGGGATGACCCAGCAGCCTCCCAGCATTAATGCGATGCGCAGAGGGGTCA
  TCTTGTTCCTATAGACCAAAGCTGGCAGCAGATGGCGTATACCTATCCAGAGAAATGCAG
  CACAGGTGAAAAATCGATGCCGTTGGGGAGCAGGACGTTCAGAGAATGTTCCG
  
• Restriction Sites: Please inquire
• ACCN: NM_199453
• Insert Size: 1300 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_199453.2, NP_955525.1
• RefSeq Size: 1557 bp
• RefSeq ORF: 1137 bp
• Locus ID: 3360
• UniProt ID: Q13639
• Cytogenetics: 5q32
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Calcium signaling pathway, Neuroactive ligand-receptor interaction
• Gene Summary: This gene is a member of the family of serotonin receptors, which are G protein coupled receptors that stimulate cAMP production in response to serotonin (5-hydroxytryptamine). The gene product is a glycosylated transmembrane protein that functions in both the peripheral and central nervous system to modulate the release of various neurotransmitters. Multiple transcript variants encoding proteins with distinct C-terminal sequences have been described. [provided by RefSeq, May 2010]
  Transcript Variant: This variant (g), also known as E or G1, differs in the 5' UTR, 3' coding region and 3' UTR, compared to variant 1. The resulting isoform (isoform g) has a distinct C-terminus and is shorter than isoform b.