WDR20 (NM_181308) Human Untagged Clone

CAT#: SC307269

WDR20 (untagged)-Human WD repeat domain 20 (WDR20), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: WDR20
• Synonyms: Bun107; DMR
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC307269 representing NM_181308.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGCGACGGAGGGAGGAGGGAAGGAGATGAACGAGATTAAGACCCAATTCACCACCCGGGAAGGTCTG
  TACAAGCTGCTGCCGCACTCGGAGTACAGCCGGCCCAACCGGGTGCCCTTCAACTCGCAGGGATCCAAC
  CCTGTCCGCGTCTCCTTCGTAAACCTCAACGACCAGTCTGGCAACGGCGACCGCCTCTGCTTCAATGTG
  GGCCGGGAGCTGTACTTCTATATCTACAAGGGGGTCCGCAAGAGACTAATAGACAAGTCACGAGTTACC
  TGTGTCAAATGGGTTCCCGGTTCGGAAAGCCTTTTCCTAGTAGCCCACTCGAGTGGGAACATGTACTTA
  TATAATGTGGAGCACACTTGTGGCACCACAGCCCCCCACTACCAGCTTCTGAAGCAGGGAGAGAGCTTT
  GCCGTGCACACTTGCAAGAGCAAATCCACGAGGAACCCTCTCCTTAAGTGGACGGTGGGCGAGGGGGCC
  CTCAACGAGTTTGCTTTCTCCCCAGATGGCAAGTTCTTAGCGTGCGTGAGCCAGGACGGGTTTCTGCGG
  GTGTTCAACTTTGACTCAGTGGAGCTGCACGGTACGATGAAAAGCTACTTTGGGGGCTTGCTGTGTGTG
  TGCTGGAGCCCGGATGGCAAGTACATCGTGACAGGTGGGGAGGACGACTTGGTGACAGTCTGGTCCTTT
  GTAGACTGCCGAGTAATAGCCAGAGGCCACGGGCACAAGTCCTGGGTCAGTGTTGTAGCGTTTGACCCT
  TATACCACTAGTGTAGAAGAAGGTGACCCTATGGAGTTTAGTGGCAGCGATGAGGACTTCCAAGACCTT
  CTTCATTTTGGCAGAGATCGAGCAAATAGTACACAGTCCAGGCTCTCCAAACGGAACTCTACAGACAGC
  CGCCCCGTAAGTGTCACGTATCGGTTTGGTTCCGTGGGCCAGGACACACAGCTCTGTTTATGGGACCTT
  ACAGAAGATATCCTTTTCCCTCACCAACCCCTCTCAAGAGCAAGGACACACACAAATGTCATGAATGCC
  ACGAGTCCTCCTGCTGGAAGCAATGGGAACAGTGTTACAACACCCGGGAACTCTGTGCCGCCTCCTCTG
  CCACGGTCCAACAGCCTTCCACATTCAGCAGTCTCAAATGCTGGCAGCAAAAGCAGTGTCATGGACGGG
  GCCATTGCTTCTGGGGTCAGCAAATTTGCAACACTTTCACTACATGACCGGAAGGAGAGGCACCACGAG
  AAAGATCACAAGCGAAATCATAGCATGGGACACATTTCTAGCAAGAGCAGTGACAAACTGAATCTAGTT
  ACCAAAACCAAAACGGACCCTGCTAAAACTCTGGGAACGCCCCTGTGTCCTCGAATGGAAGATGTTCCC
  TTGTTAGAGCCGCTGATATGTAAAAAGATAGCACATGAGAGACTGACTGTACTAATATTTCTTGAAGAC
  TGTATAGTCACTGCTTGTCAGGAGGGATTTATTTGCACATGGGGAAGGCCTGGTAAAGTGGTAAGTTTT
  AATCCTTAA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_181308
• Insert Size: 1527 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_181308.2
• RefSeq Size: 2263 bp
• RefSeq ORF: 1527 bp
• Locus ID: 91833
• UniProt ID: Q8TBZ3
• Cytogenetics: 14q32.31
• MW: 56.2 kDa
• Gene Summary: This gene encodes a WD repeat-containing protein that functions to preserve and regulate the activity of the USP12-UAF1 deubiquitinating enzyme complex. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jun 2011]
  Transcript Variant: This variant (3) lacks an alternate in-frame exon in the central coding region and differs in the 3' coding region and 3' UTR, compared to variant 1. The encoded isoform (3) has a distinct C-terminus and is shorter than isoform 1.