Myosin 1G (MYO1G) (NM_033054) Human Untagged Clone

CAT#: SC305543

MYO1G (untagged)-Human myosin IG (MYO1G)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Myosin 1G
• Synonyms: HA2; HLA-HA2; MHAG
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC305543 representing NM_033054.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGAGGACGAGGAAGGCCCTGAGTATGGCAAACCTGACTTTGTGCTTTTGGACCAAGTGACCATGGAG
  GACTTCATGAGGAACCTGCAGCTCAGGTTCGAGAAGGGCCGCATCTACACCTACATCGGTGAGGTGCTG
  GTGTCCGTGAACCCCTACCAGGAGCTGCCCCTGTATGGGCCTGAGGCCATCGCCAGGTACCAGGGCCGT
  GAGCTCTATGAGCGGCCACCCCATCTCTATGCTGTGGCCAACGCCGCCTACAAGGCAATGAAGCACCGG
  TCCAGGGACACCTGCATCGTCATCTCAGGGGAGAGTGGGGCAGGGAAGACAGAAGCCAGTAAGCACATC
  ATGCAGTACATCGCTGCTGTCACCAATCCAAGCCAGAGGGCTGAGGTGGAGAGGGTCAAGGACGTGCTG
  CTCAAGTCCACCTGTGTGCTGGAGGCCTTTGGCAATGCCCGCACCAACCGCAATCACAACTCCAGCCGC
  TTTGGCAAGTACATGGACATCAACTTTGACTTCAAGGGGGACCCGATCGGAGGACACATCCACAGCTAC
  CTACTGGAGAAGTCTCGGGTCCTCAAGCAGCACGTGGGTGAAAGAAACTTCCACGCCTTCTACCAATTG
  CTGAGAGGCAGTGAGGACAAGCAGCTGCATGAACTGCACTTGGAGAGAAACCCTGCTGTATACAATTTC
  ACACACCAGGGAGCAGGACTCAACATGACTGTGCACAGTGCCTTGGACAGTGATGAGCAGAGCCACCAG
  GCAGTGACCGAGGCCATGAGGGTCATCGGCTTCAGTCCTGAAGAGGTGGAGTCTGTGCATCGCATCCTG
  GCTGCCATATTGCACCTGGGAAACATCGAGTTTGTGGAGACGGAGGAGGGTGGGCTGCAGAAGGAGGGC
  CTGGCAGTGGCCGAGGAGGCACTGGTGGACCATGTGGCTGAGCTGACGGCCACACCCCGGGACCTCGTG
  CTCCGCTCCCTGCTGGCTCGCACAGTTGCCTCGGGAGGCAGGGAACTCATAGAGAAGGGCCACACTGCA
  GCTGAGGCCAGCTATGCCCGGGATGCCTGTGCCAAGGCAGTGTACCAGCGGCTGTTTGAGTGGGTGGTG
  AACAGGATCAACAGTGTCATGGAACCCCGGGGCCGGGATCCTCGGCGTGATGGCAAGGACACAGTCATT
  GGCGTGCTGGACATCTATGGCTTCGAGGTGTTTCCCGTCAACAGTTTCGAGCAGTTCTGCATCAACTAC
  TGCAATGAGAAGCTGCAGCAGCTATTCATCCAGCTCATCCTGAAGCAGGAACAGGAAGAGTACGAGCGC
  GAGGGCATCACCTGGCAGAGCGTTGAGTATTTCAACAACGCCACCATTGTGGATCTGGTGGAGCGGCCC
  CACCGTGGCATCCTGGCCGTGCTGGACGAGGCCTGCAGCTCTGCTGGCACCATCACTGACCGAATCTTC
  CTGCAGACCCTGGACACGCACCACCGCCATCATCTACACTACACCAGCCGCCAGCTCTGCCCCACAGAC
  AAGACCATGGAGTTTGGCCGAGACTTCCGGATCAAGCACTATGCAGGGGACGTCACGTACTCCGTGGAA
  GGCTTCATCGACAAGAACAGAGATTTCCTCTTCCAGGACTTCAAGCGGCTGCTGTACAACAGCACGGAC
  CCCACTCTACGGGCCATGTGGCCGGACGGGCAGCAGGACATCACAGAGGTGACCAAGCGCCCCCTGACG
  GCTGGCACACTCTTCAAGAACTCCATGGTGGCCCTGGTGGAGAACCTTGCCTCCAAGGAGCCCTTCTAC
  GTCCGCTGCATCAAGCCCAATGAGGACAAGGTAGCTGGGAAGCTGGATGAGAACCACTGTCGCCACCAG
  GTCGCATACCTGGGGCTGCTGGAGAATGTGAGGGTCCGCAGGGCTGGCTTCGCTTCCCGCCAGCCCTAC
  TCTCGATTCCTGCTCAGGTACAAGATGACCTGTGAATACACATGGCCCAACCACCTGCTGGGCTCCGAC
  AAGGCAGCCGTGAGCGCTCTCCTGGAGCAGCATGGGCTGCAGGGGGACGTGGCCTTTGGCCACAGCAAG
  CTGTTCATCCGCTCACCCCGGACACTGGTCACACTGGAGCAGAGCCGAGCCCGCCTCATCCCCATCATT
  GTGCTGCTATTGCAGAAGGCATGGCGGGGCACCTTGGCGAGGTGGCGCTGCCGGAGGCTGAGGGCTATC
  TACACCATCATGCGCTGGTTCCGGAGACACAAGGTGCGGGCTCACCTGGCTGAGCTGCAGCGGCGATTC
  CAGGCTGCAAGGCAGCCGCCACTCTACGGGCGTGACCTTGTGTGGCCGCTGCCCCCTGCTGTGCTGCAG
  CCCTTCCAGGACACCTGCCACGCACTCTTCTGCAGGTGGCGGGCCCGGCAGCTGGTGAAGAACATCCCC
  CCTTCAGACATGCCCCAGATCAAGGCCAAGGTGGCCGCCATGGGGGCCCTGCAAGGGCTTCGTCAGGAC
  TGGGGCTGCCGACGGGCCTGGGCCCGAGACTACCTGTCCTCTGCCACTGACAATCCCACAGCATCAAGC
  CTGTTTGCTCAGCGACTAAAGACACTTCGGGACAAAGATGGCTTCGGGGCTGTGCTCTTTTCAAGCCAT
  GTCCGCAAGGTGAACCGCTTCCACAAGATCCGGAACCGGGCCCTCCTGCTCACAGACCAGCACCTCTAC
  AAGCTGGACCCTGACCGGCAGTACCGGGTGATGCGGGCCGTGCCCCTTGAGGCGGTGACGGGGCTGAGC
  GTGACCAGCGGAGGAGACCAGCTGGTGGTGCTGCACGCCCGCGGCCAGGACGACCTCGTGGTGTGCCTG
  CACCGCTCCCGGCCGCCATTGGACAACCGCGTTGGGGAGCTGGTGGGCGTGCTGGCCGCACACTGCCAG
  GGGGAGGGCCGCACCCTGGAGGTTCGCGTCTCCGACTGCATCCCACTAAGCCATCGCGGGGTCCGGCGC
  CTCATCTCCGTGGAGCCCAGGCCGGAGCAGCCAGAGCCCGATTTCCGCTGCGCTCGCGGCTCCTTCACC
  CTGCTCTGGCCCAGCCGCTGA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_033054
• Insert Size: 3057 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_033054.1
• RefSeq Size: 3313 bp
• RefSeq ORF: 3057 bp
• Locus ID: 64005
• UniProt ID: B0I1T2
• Cytogenetics: 7p13
• MW: 116.4 kDa
• Gene Summary: MYO1G is a plasma membrane-associated class I myosin (see MIM 601478) that is abundant in T and B lymphocytes and mast cells (Pierce et al., 2001 [PubMed 11544309]; Patino-Lopez et al., 2010 [PubMed 20071333]).[supplied by OMIM, Jun 2010]