PCDHGA1 (NM_031993) Human Untagged Clone

CAT#: SC305383

PCDHGA1 (untagged)-Human protocadherin gamma subfamily A, 1 (PCDHGA1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PCDHGA1
• Synonyms: PCDH-GAMMA-A1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC305383 representing NM_031993.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGAAGATTCAGAAAAAGCTGACTGGCTGCAGCAGGCTGATGCTTCTGTGTCTTTCTCTGGAGCTGCTG
  TTGGAAGCTGGGGCTGGGAATATTCACTACTCAGTGCCGGAAGAGACAGACAAAGGTTCCTTCGTAGGC
  AACATCGCCAAGGACCTAGGGCTGCAACCCCAGGAGCTGGCAGATGGCGGAGTCCGCATCGTCTCCAGA
  GGTAGGATGCCGCTTTTCGCTCTGAATCCTAGAAGTGGCAGCTTGATCACCGCGCGCAGGATAGACCGG
  GAGGAGCTCTGCGCTCAGAGCATGCCGTGTCTCGTGAGTTTTAATATCCTTGTTGAGGATAAAATGAAG
  CTTTTTCCTGTTGAAGTAGAAATAATTGATATTAATGACAACACTCCCCAATTCCAGTTAGAGGAACTG
  GAGTTTAAAATGAATGAAATAACGACTCCAGGTACCAGAGTCTCATTGCCTTTTGGGCAAGACCTTGAT
  GTGGGTATGAACTCACTCCAGAGCTACCAACTCAGCTCTAACCCTCATTTCTCCCTGGATGTGCAACAG
  GGAGCCGATGGGCCTCAACATCCAGAGATGGTGCTGCAGAGTCCCTTAGACAGAGAAGAAGAAGCTGTC
  CACCACCTCATCCTCACAGCTTCTGATGGGGGTGAACCAGTCCGTTCAGGGACCCTCAGAATTTACATT
  CAGGTGGTGGATGCAAATGACAATCCTCCAGCATTTACTCAGGCACAATACCATATAAATGTCCCCGAA
  AACGTGCCGCTGGGTACTCAGCTGCTCATGGTAAATGCCACTGACCCTGATGAGGGAGCCAATGGGGAA
  GTAACGTACTCCTTTCACAATGTAGACCACAGAGTGGCCCAAATATTTCGTTTAGATTCTTACACAGGA
  GAAATATCAAATAAAGAACCACTAGATTTCGAAGAATACAAAATGTATTCAATGGAAGTTCAAGCCCAG
  GATGGTGCGGGGCTCATGGCTAAAGTTAAGGTACTGATCAAAGTTTTGGATGTAAATGATAATGCCCCA
  GAAGTGACCATCACCTCTGTCACCACTGCAGTTCCAGAAAACTTTCCTCCTGGGACCATAATTGCTCTT
  ATCAGTGTGCATGACCAGGACTCAGGAGACAATGGCTACACCACATGTTTCATTCCTGGAAATTTACCC
  TTTAAATTGGAAAAGTTAGTTGATAATTATTACCGTTTAGTGACTGAAAGAACACTGGACAGAGAACTT
  ATCTCTGGGTACAACATCACAATAACAGCAATAGACCAAGGAACTCCAGCTCTATCTACTGAAACTCAC
  ATTTCACTACTAGTGACAGATATCAATGACAACTCCCCAGTCTTCCATCAGGACTCCTACTCTGCCTAC
  ATTCCCGAAAACAACCCCAGAGGAGCCTCCATCTTCTCTGTGAGGGCCCACGACTTGGACAGCAATGAG
  AATGCACAAATCACTTACTCCCTAATAGAGGACACTATCCAGGGGGCACCCCTATCTGCCTACCTCTCC
  ATCAACTCCGACACTGGGGTCCTGTATGCGCTGCGATCCTTCGACTATGAGCAGTTCCGGGACATGCAA
  CTGAAAGTGATGGCGCGGGACAGTGGGGATCCGCCCCTCAGCAGCAACGTGTCTCTCAGCCTATTCCTG
  CTGGACCAGAACGACAACGCGCCCGAGATCCTGTACCCCGCCCTCCCCACAGATGGTTCTACCGGCGTG
  GAGCTGGCGCCCCTCTCCGCAGAGCCCGGCTACCTGGTGACCAAGGTGGTGGCGGTGGACAGAGACTCG
  GGCCAGAACGCCTGGCTGTCCTACCGCCTGCTCAAGGCCAGCGAGCCGGGACTCTTCTCGGTGGGTCTG
  CACACGGGCGAGGTGCGCACGGCGCGAGCCCTGCTGGACAGAGACGCGCTCAAGCAGAGTCTCGTGGTG
  GCCGTCCAGGACCACGGCCAGCCCCCGCTCTCCGCCACTGTCACGCTCACCGTGGCCGTGGCCGACAGG
  ATCTCCGACATCCTGGCCGACCTGGGCAGCCTCGAGCCCTCCGCCAAACCCAACGATTCGGACCTCACT
  CTGTACCTGGTGGTGGCGGCGGCCGCGGTCTCCTGCGTCTTCCTGGCCTTCGTCATCGTGCTGCTGGCG
  CACAGGCTGCGGCGCTGGCACAAGTCACGTCTGCTACAGGCTTCGGGAGGCGGCTTAGCGAGCATGCCC
  GGTTCGCACTTTGTGGGCGTGGACGGGGTTCGGGCTTTCCTGCAGACCTATTCCCACGAGGTCTCCCTC
  ACTGCGGACTCGCGGAAGAGCCACCTGATTTTCCCCCAGCCCAACTATGCGGACACACTCATCAGCCAG
  GAGAGCTGTGAGAAAAAGGGTTTTCTATCAGCACCCCAGTCTTTACTTGAAGACAAAAAGGAACCATTT
  TCTCAGGTAAACTTTTGTGATGAATGTATCAGCTATCTAGAGAAAAATAATTCTTGA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_031993
• Insert Size: 2472 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.

Reference Data

• RefSeq: NM_031993.1
• RefSeq Size: 2472 bp
• RefSeq ORF: 2472 bp
• Locus ID: 56114
• UniProt ID: Q9Y5H4
• Cytogenetics: 5q31.3
• Protein Families: Transmembrane
• MW: 90.2 kDa
• Gene Summary: This gene is a member of the protocadherin gamma gene cluster, one of three related clusters tandemly linked on chromosome five. These gene clusters have an immunoglobulin-like organization, suggesting that a novel mechanism may be involved in their regulation and expression. The gamma gene cluster includes 22 genes divided into 3 subfamilies. Subfamily A contains 12 genes, subfamily B contains 7 genes and 2 pseudogenes, and the more distantly related subfamily C contains 3 genes. The tandem array of 22 large, variable region exons are followed by a constant region, containing 3 exons shared by all genes in the cluster. Each variable region exon encodes the extracellular region, which includes 6 cadherin ectodomains and a transmembrane region. The constant region exons encode the common cytoplasmic region. These neural cadherin-like cell adhesion proteins most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. Alternative splicing has been described for the gamma cluster genes. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) utilizes the large, first exon then continues into the downstream intron 1 sequence before terminating. This one-exon transcript encodes the shorter isoform (2), compared to isoform 1. This short variant is represented based on data in PMID:10380929.