RNF150 (NM_020724) Human Untagged Clone

CAT#: SC304807

RNF150 (untagged)-Human ring finger protein 150 (RNF150)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RNF150
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC304807 representing NM_020724.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGCAATGTCTCTCATCCAAGCGTGCTGCAGTCTGGCTCTCTCAACATGGCTGCTTTCCTTTTGTTTC
  GTGCATCTGCTCTGCCTGGACTTTACCGTGGCCGAGAAGGAGGAATGGTACACCGCCTTCGTGAACATC
  ACCTACGCCGAGCCCGCGCCGGACCCCGGGGCCGGGGCGGCGGGCGGCGGCGGCGCGGAGCTGCACACG
  GAGAAGACGGAGTGCGGGCGCTACGGAGAGCACTCGCCCAAGCAGGACGCCCGCGGGGAGGTGGTCATG
  GCCAGCTCGGCCCACGACCGCCTGGCCTGCGACCCCAACACCAAGTTCGCCGCCCCGACCCGCGGCAAG
  AACTGGATAGCCCTCATCCCCAAGGGCAACTGCACGTACAGGGATAAGATCCGGAACGCGTTCCTGCAG
  AACGCCTCAGCCGTGGTCATCTTCAACGTGGGCTCCAACACCAACGAGACCATCACCATGCCCCACGCG
  GGTGTAGAAGACATCGTGGCCATAATGATTCCTGAGCCAAAAGGGAAGGAGATAGTAAGCCTGCTGGAA
  AGAAACATCACCGTGACAATGTACATCACCATCGGAACCCGGAACTTGCAGAAATATGTGAGCCGCACT
  TCGGTTGTGTTTGTCTCCATCTCCTTCATTGTCCTGATGATCATTTCCCTCGCATGGCTCGTCTTTTAT
  TACATCCAGAGGTTTCGATATGCAAATGCCAGGGATAGGAACCAGCGCCGACTGGGGGATGCAGCAAAG
  AAAGCCATCAGCAAACTCCAGATCAGGACCATCAAGAAGGGTGACAAGGAAACAGAGTCTGATTTTGAC
  AACTGTGCAGTTTGTATTGAAGGGTACAAGCCCAATGACGTTGTCCGGATCCTGCCCTGCCGGCATCTT
  TTCCACAAGTCCTGTGTTGACCCCTGGCTTCTAGACCATCGTACCTGTCCCATGTGCAAGATGAACATT
  CTTAAAGCCCTAGGGATCCCGCCCAATGCCGACTGCATGGACGACTTGCCCACTGACTTCGAGGGCTCT
  CTGGGAGGTCCACCCACCAACCAGATCACAGGTGCCAGCGACACAACAGTGAATGAAAGTTCAGTCACT
  TTGGACCCTGCTGTCCGGACTGTGGGAGCCTTGCAGGTGGTCCAGGATACAGACCCCATCCCCCAGGAG
  GGAGACGTCATCTTTACTACTAACAGTGAGCAGGAGCCAGCTGTAAGCAGTGATTCTGACATTTCCTTG
  ATCATGGCAATGGAGGTTGGACTGTCTGATGTAGAACTTTCCACTGACCAGGACTGTGAAGAAGTGAAA
  TCTTGA
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGAT
  ATCCTGGATTACAAGGATGACGACGATAAGGTTTAA
  
• Restriction Sites: SgfI-RsrII
• ACCN: NM_020724
• Insert Size: 1317 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020724.1
• RefSeq Size: 4661 bp
• RefSeq ORF: 1317 bp
• Locus ID: 57484
• UniProt ID: Q9ULK6
• Cytogenetics: 4q31.21
• Protein Families: Druggable Genome, Transmembrane
• MW: 48.1 kDa