ANKRD50 (NM_020337) Human Untagged Clone

CAT#: SC304740

ANKRD50 (untagged)-Human ankyrin repeat domain 50 (ANKRD50), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ANKRD50
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC304740 representing NM_020337.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGACTAATCCTTGGGAAGAGAAAGTCTGCAAAATGGCTCAAACCAGTTTACTGCAAGGGAAGCAGTTT
  TACTGTAGGGAGTGGGTTTTCCACAAGCTTCAGCATTGCCTCCAGGAGAAAAGTAACTGCTGCAATAGT
  GCTGTCAATGCACCATCACTTGTAATGAATTCTGGGAATAATGCTAGTGGTGTCTCTGGAAAGGGAGCT
  GCCTGGGGTGTGTTGTTGGTAGGAGGGCCTGGCAGTGGCAAGACGGCCCTATGTACTGAACTCTTATGG
  CCAAGTTCACCTGCAAGTTTGCAGAGAGGTTTACATCGCCAAGCTTTGGCCTTTCATTTCTGCAAAGCC
  CAGGACTCTGATACTTTGTGTGTTGGAGGGTTTATTAGAGGTCTAGTAGCCCAGATCTGCCGCAGTGGA
  CTACTCCAAGGATATGAGGACAAGCTAAGGGATCCAGCAGTCCAAAGCCTCTTACAGCCTGGGGAGTGC
  GAGAGAAACCCAGCCGAAGCATTTAAAAGGTGTGTTCTACTCCCTCTTCTGGGAATGAAGCCTCCCCAG
  CAAAGCCTATACCTGCTTGTTGATTCTGTTGATGAAGGGTGTAACATTACTGAAGGTGAACAAACGTCT
  ACCAGCTTATCTGGGACTGTTGCAGCACTTTTAGCTGGTCACCATGAGTTCTTTCCACCATGGCTATTG
  CTTCTCTGTTCTGCCCGAAAGCAGAGTAAGGCTGTTACTAAAATGTTTACTGGTTTTCGAAAAATAAGT
  TTAGATGACCTTCGGAAGGCATATATCGTCAAGGATGTTCAGCAGTACATTCTTCATCGTTTAGATCAA
  GAAGAAGCTTTGCGACAACACCTCACAAAAGAAACTGCAGAGATGTTAAATCAACTGCACATTAAAAGC
  AGTGGATGCTTTCTTTACCTAGAACGAGTTTTAGATGGAGTTGTAGAAAATTTTATTATGTTAAGAGAA
  ATTCGTGACATCCCAGGAACTCTAAATGGTTTATATCTCTGGCTGTGCCAAAGACTTTTTGTAAGAAAA
  CAATTTGCAAAGGTTCAGCCTATTTTGAATGTGATTCTTGCAGCCTGCCGACCTTTGACCATAACGGAA
  TTATATCACGCAGTATGGACCAAAAACATGTCGTTAACTTTGGAAGATTTTCAACGCAAGTTAGATATC
  CTCTCCAAACTTCTTGTTGATGGACTAGGAAATACAAAAATACTGTTTCATTATAGTTTTGCCGAGTGG
  CTTCTGGATGTGAAACACTGTACTCAGAAGTATTTATGTAATGCAGCAGAAGGACACAGAATGTTGGCT
  ATGAGTTATACCTGTCAAGCCAAGAATTTAACACCATTGGAAGCACAAGAATTTGCATTGCACTTAATT
  AACTCAAACTTACAATTAGAGACAGCGGAGTTAGCTCTGTGGATGATATGGAATGGTACACCTGTCAGA
  GATTCCCTTTCTACTTTGATACCCAAGGAACAAGAAGTGCTACAGCTGTTGGTTAAAGCTGGGGCTCAT
  GTCAACAGTGAAGACGATCGCACATCATGCATAGTTCGACAAGCCTTAGAAAGAGAGGATTCCATTCGG
  ACATTATTAGATAATGGAGCTTCAGTAAATCAGTGTGATTCAAATGGGAGAACATTATTGGCTAATGCT
  GCATATAGTGGCAGTCTTGATGTAGTCAATTTACTTGTCTCTAGGGGAGCAGATTTAGAGATAGAAGAT
  GCTCATGGACATACACCACTCACTCTAGCGGCTAGACAGGGACATACCAAGGTGGTTAATTGTTTGATT
  GGGTGTGGAGCAAATATTAATCATACTGATCAAGATGGTTGGACAGCATTAAGATCTGCTGCTTGGGGT
  GGCCATACTGAGGTAGTTTCTGCACTACTTTATGCTGGCGTAAAAGTGGATTGTGCAGATGCTGATAGC
  CGAACAGCTTTGAGAGCAGCAGCATGGGGAGGACACGAGGATATTGTACTGAATTTGCTACAACATGGC
  GCTGAAGTGAACAAAGCTGATAATGAAGGTAGAACTGCTTTGATAGCAGCAGCATACATGGGACATAGA
  GAGATTGTGGAACACCTACTGGACCATGGAGCAGAAGTAAATCATGAGGATGTTGATGGCAGGACTGCA
  CTCTCTGTAGCTGCACTTTGTGTGCCTGCAAGTAAAGGGCACGCATCAGTTGTTAGCCTTTTAATTGAT
  CGAGGTGCTGAAGTAGATCATTGTGATAAAGATGGCATGACTCCACTGCTGGTAGCTGCCTATGAAGGA
  CATGTTGATGTGGTTGACTTGCTTCTAGAAGGGGGAGCAGATGTAGATCACACAGATAACAATGGCCGT
  ACACCCCTCTTAGCAGCAGCGTCTATGGGTCATGCATCAGTTGTAAATACACTTTTGTTTTGGGGTGCA
  GCTGTGGATAGTATTGATAGTGAAGGTAGGACAGTCCTCAGTATAGCTTCAGCACAAGGAAATGTTGAG
  GTGGTACGTACTCTACTGGATAGAGGGTTAGATGAAAATCACAGAGATGATGCTGGATGGACACCTTTG
  CACATGGCAGCTTTTGAAGGGCACAGATTGATATGTGAAGCACTTATTGAACAAGGTGCTAGAACAAAT
  GAGATTGACAATGATGGACGAATCCCTTTCATATTAGCTTCACAAGAGGGTCATTATGATTGTGTTCAA
  ATATTACTGGAAAACAAATCCAACATTGATCAAAGAGGTTATGATGGAAGAAATGCACTGCGGGTTGCT
  GCATTAGAAGGGCACAGGGACATTGTTGAATTGCTTTTTAGCCATGGTGCTGATGTTAACTGCAAAGAT
  GCTGATGGTCGGCCTACACTTTATATCTTGGCCTTAGAAAATCAGCTTACAATGGCCGAATATTTTTTA
  GAAAATGGTGCAAACGTAGAAGCAAGTGATGCTGAAGGAAGGACAGCACTTCATGTGTCTTGTTGGCAA
  GGCCATATGGAAATGGTGCAGGTCCTGATAGCATACCATGCTGACGTCAATGCTGCAGACAATGAAAAG
  CGCTCTGCTTTGCAGTCTGCAGCCTGGCAGGGCCATGTAAAAGTGGTTCAGCTTCTGATTGAGCATGGT
  GCTGTAGTTGACCATACATGTAACCAAGGTGCAACTGCACTCTGTATTGCAGCCCAGGAAGGGCACATT
  GATGTTGTTCAGGTCTTATTAGAGCATGGTGCTGATCCAAACCATGCTGATCAATTTGGACGCACTGCT
  ATGCGTGTTGCAGCCAAAAATGGACATTCTCAGATAATTAAATTATTAGAAAAATATGGTGCATCTAGT
  TTGAATGGCTGTTCCCCATCTCCTGTTCACACAATGGAGCAAAAACCTCTACAGTCATTGTCTTCAAAA
  GTGCAGTCATTAACAATTAAATCAAATAGCTCTGGTAGTACTGGTGGAGGGGATATGCAGCCTTCGTTA
  CGTGGTTTACCTAATGGGCCTACTCATGCTTTTAGTTCTCCTTCAGAATCTCCAGATTCTACAGTTGAC
  CGGCAGAAGTCATCACTGTCAAATAATTCCCTGAAAAGCTCAAAAAATTCATCTTTGAGAACTACTTCA
  TCTACAGCAACGGCTCAAACAGTGCCAATTGATAGCTTTCATAACTTGTCATTTACAGAACAAATTCAG
  CAGCATTCATTGCCACGCAGTAGAAGTCGACAGTCAATTGTTTCCCCATCTTCCACAACACAGTCCTTA
  GGACAGAGTCATAATTCACCAAGTAGTGAATTTGAGTGGAGTCAAGTAAAGCCCAGTTTGAAGTCAACT
  AAAGCAAGTAAAGGGGGGAAATCAGAAAATTCTGCCAAGTCTGGATCAGCTGGGAAAAAAGCGAAACAA
  AGTAATTCTTCACAGCCAAAGGTTTTAGAATATGAAATGACTCAGTTTGATAGAAGAGGACCTATAGCC
  AAATCCGGGACTGCTGCACCACCTAAACAAATGCCAGCAGAATCTCAATGCAAAATTATGATACCTTCA
  GCTCAGCAGGAAATTGGTCGATCTCAACAGCAGTTTCTTATTCACCAACAAAGTGGGGAACAGAAGAAG
  AGAAATGGAATAATGACAAATCCAAATTATCATCTTCAGAGCAACCAGGTTTTTCTTGGTAGGGTTTCA
  GTCCCACGAACAATGCAAGATAGAGGGCATCAGGAAGTGTTGGAGGGATACCCTTCCTCAGAGACAGAA
  TTAAGCCTTAAACAAGCTCTGAAGCTTCAGATTGAAGGTTCTGACCCTAGCTTCAACTATAAAAAGGAA
  ACACCATTATAA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_020337
• Insert Size: 4290 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020337.2
• RefSeq Size: 8802 bp
• RefSeq ORF: 4290 bp
• Locus ID: 57182
• UniProt ID: Q9ULJ7
• Cytogenetics: 4q28.1
• MW: 155.9 kDa
• Gene Summary: Involved in the endosome-to-plasma membrane trafficking and recycling of SNX27-retromer-dependent cargo proteins, such as GLUT1 (PubMed:25278552).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes the longer isoform (1).