EPS8L1 (NM_017729) Human Untagged Clone

CAT#: SC304519

EPS8L1 (untagged)-Human EPS8-like 1 (EPS8L1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: EPS8L1
• Synonyms: DRC3; EPS8R1; PP10566
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC304519 representing NM_017729.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGAACAGAACATGGCCAAGAAGGATCTGGGGGAGCAGCCAGGACGAGGCGGAGCTGATCCGAGAGGAC
  ATCCAGGGGGCTCTGCACAATTACCGCTCGGGCCGCGGGGAGCGCAGGGCGGCGGCGCTCAGGGCCACG
  CAGGAGGAGTTGCAGCGCGACCGCTCGCCCGCCGCTGAGACCCCGCCCCTGCAGCGCCGCCCGTCAGTC
  CGCGCAGTGATCAGCACCGTAGAGCGGGGCGCGGGCCGCGGACGACCCCAGGCGAAGCCCATTCCCGAG
  GCAGAGGAGGCGCAGAGGCCTGAGCCGGTGGGGACCTCGAGCAACGCTGACTCGGCCTCCCCGGACCTG
  GGTCCCCGGGGTCCTGACCTGGCGGTTCTGCAGGCGGAGCGGGAAGTGGACATCCTGAACCACGTGTTC
  GACGACGTAGAGAGCTTTGTATCGAGGCTGCAGAAGTCGGCGGAGGCGGCCAGGGTGCTGGAGCACCGG
  GAACGCGGCCGCAGGAGCCGGCGCCGGGCGGCTGGGGAGGGCTTGCTGACGCTGCGGGCCAAGCCGCCC
  TCGGAGGCCGAGTACACCGACGTGCTGCAGAAGATCAAGTACGCCTTCAGCCTGCTGGCCCGGCTGCGC
  GGCAACATCGCCGACCCCTCCTCTCCGGAGCTGTTGCACTTCCTTTTCGGGCCTCTGCAGATGATTGTG
  AACACGTCGGGGGGGCCGGAGTTCGCGAGCAGTGTGCGGCGGCCGCATCTGACATCGGATGCCGTGGCG
  CTGCTGCGGGACAACGTCACTCCACGTGAAAACGAGCTCTGGACCTCGCTGGGGGACTCGTGGACCCGC
  CCCGGGCTGGAGCTGTCCCCGGAGGAGGGACCCCCATACAGACCCGAGTTCTTCAGCGGCTGGGAGCCG
  CCGGTCACTGACCCGCAGAGCCGCGCCTGGGAGGACCCAGTTGAGAAACAGCTACAGCACGAGCGGAGG
  CGCCGGCAGCAAAGCGCCCCCCAGGTCGCTGTCAATGGTCACCGAGACTTGGAGCCAGAATCTGAGCCT
  CAGCTGGAGTCAGAGACAGCAGGAAAATGGGTCCTGTGTAATTATGACTTCCAGGCCCGCAACAGCAGT
  GAGCTGTCGGTCAAGCAGCGGGACGTACTGGAGGTCCTGGATGACAGTCGTAAGTGGTGGAAGGTTCGG
  GACCCAGCGGGGCAGGAGGGATATGTGCCCTACAACATCCTGACACCCTACCCCGGACCCCGGCTGCAC
  CACAGCCAAAGCCCTGCCCGCAGCCTGAACAGCACTCCTCCTCCACCACCAGCCCCAGCCCCGGCCCCA
  CCTCCAGCTCTGGCTCGGCCCCGCTGGGACAGGCCCCGCTGGGACAGCTGCGATAGCCTCAACGGCTTG
  GACCCCAGCGAGAAGGAGAAATTCTCCCAGATGCTCATCGTCAACGAGGAACTGCAGGCGCGCCTGGCC
  CAGGGCCGCTCGGGACCGAGCCGCGCAGTCCCAGGGCCCCGCGCCCCGGAACCGCAGCTCAGCCCGGGC
  TCGGACGCCTCCGAGGTCCGCGCCTGGCTGCAGGCCAAGGGCTTTAGCTCCGGGACCGTGGACGCGCTG
  GGTGTGCTGACCGGGGCGCAGCTTTTCTCGCTGCAGAAGGAGGAGCTGCGGGCGGTGAGCCCCGAGGAG
  GGGGCACGTGTGTACAGCCAGGTCACCGTGCAGCGCTCGCTGCTGGAGGACAAAGAGAAAGTGTCAGAG
  CTGGAGGCAGTGATGGAGAAGCAAAAGAAGAAGGTGGAAGGCGAGGTGGAAATGGAGGTCATTTGA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_017729
• Insert Size: 1791 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017729.3
• RefSeq Size: 2224 bp
• RefSeq ORF: 1791 bp
• Locus ID: 54869
• UniProt ID: Q8TE68
• Cytogenetics: 19q13.42
• Protein Families: Druggable Genome
• MW: 66.5 kDa
• Gene Summary: This gene encodes a protein that is related to epidermal growth factor receptor pathway substrate 8 (EPS8), a substrate for the epidermal growth factor receptor. The function of this protein is unknown. At least two alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) differs in the 5' UTR and contains an alternate in-frame segment in the 5' coding region compared to variant 1. It encodes a shorter isoform (b) with a different N-terminus compared to isoform a.