DENND2A (NM_015689) Human Untagged Clone

CAT#: SC304343

DENND2A (untagged)-Human DENN/MADD domain containing 2A (DENND2A)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DENND2A
• Synonyms: FAM31D; KIAA1277
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC304343 representing NM_015689.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGATATGTTCAGCTTGGATATGATCATCAGTGACCCAGCTGCAGAAGCCAGCAGGGCTGGGAAGAAG
  CAGCTCAGAGGTGTTCAGAACCCTTGCCCATCTGCCAGAGCCAGACCCCGGCACAAGTCCCTCAACATA
  AAGGACAAGATATCAGAATGGGAAGGGAAGAAAGAGGTGCCCACTCCTGCACCCAGCAGGAGAGCAGAC
  GGACAGGAGGATTATCTGCCGTCCTCTACGGTGGAGAGGAGGAGTAGTGATGGGGTGAGAACTCAGGTC
  ACAGAGGCTAAGAATGGAATGAGGCCAGGAACAGAGAGCACAGAGAAGGAGAGGAATAAAGGAGCAGTG
  AACGTCGGGGGACAGGACCCAGAGCCGGGGCAAGACCTAAGCCAGCCAGAACGGGAAGTGGATCCTAGC
  TGGGGCCGAGGCCGAGAGCCAAGACTTGGCAAGCTACGCTTTCAGAACGATCACCTCTCCGTGCTGAAG
  CAGGTCAAGAAACTCGAGCAGGCTTTGAAGGATGGGTCGGCAGGGCTGGATCCCCAGTTACCAGGGACT
  TGTTACTCCCCACACTGCCCTCCTGACAAGGCAGAGGCAGGGTCCACCCTTCCTGAGAACCTGGGAGGC
  GGGAGTGGCTCAGAAGTCAGCCAGAGGGTCCACCCCTCGGACCTGGAAGGCAGGGAGCCCACCCCTGAG
  CTTGTGGAGGACAGGAAAGGTTCATGCAGAAGGCCCTGGGACCGGAGCCTTGAGAACGTGTATAGGGGC
  TCGGAGGGTTCCCCCACAAAGCCCTTCATCAACCCTCTGCCAAAACCCCGGAGAACGTTCAAACATGCC
  GGAGAAGGGGACAAAGATGGGAAGCCTGGCATCGGCTTCAGGAAAGAGAAAAGAAATCTGCCTCCTCTG
  CCCTCTCTACCTCCCCCGCCTCTGCCCTCCTCTCCCCCACCTTCCTCTGTGAACAGAAGACTGTGGACC
  GGGAGACAGAAATCCAGTGCAGACCACAGAAAGTCCTATGAGTTTGAAGATTTACTGCAGTCTTCCTCT
  GAGAGCAGCAGGGTGGACTGGTACGCGCAGACTAAGCTGGGGCTGACACGCACTTTATCGGAGGAGAAC
  GTCTATGAAGACATTCTAGATCCGCCAATGAAGGAGAACCCTTATGAGGACATCGAGTTACATGGTCGC
  TGCCTGGGAAAGAAGTGTGTCTTGAATTTTCCTGCTTCTCCCACCTCTTCCATCCCTGACACACTCACC
  AAGCAGTCATTGTCCAAACCTGCTTTTTTCCGACAAAATTCAGAGAGGAGGAACTTCAAGCTGCTGGAC
  ACTAGGAAGCTGAGTCGGGATGGAACTGGGTCCCCTTCCAAAATCAGCCCTCCCTCCACTCCCAGCAGC
  CCTGATGACATTTTCTTTAACCTTGGAGACCCACAGAACGGCAGGAAGAAGAGAAAGATACCCAAGCTG
  GTGTTGCGAATCAACGCCATTTATGAGGTCCGGAGAGGAAAGAAACGGGTGAAGAGGCTGTCCCAGTCA
  ATGGAGAGCAACTCAGGAAAAGTGACAGATGAGAACAGTGAGTCTGACAGTGACACAGAGGAGAAGCTG
  AAAGCTCACAGCCAGCGCCTGGTCAACGTGAAGTCCCGGCTGAAGCAGGCGCCTCGGTACCCATCACTT
  GCCCGGGAACTCATCGAGTACCAGGAGAGGCAGCTCTTCGAGTACTTTGTGGTTGTGTCTTTGCACAAG
  AAGCAGGCCGGGGCTGCCTACGTGCCAGAACTCACCCAACAGTTCCCTCTGAAGTTGGAAAGGTCTTTC
  AAGTTCATGAGAGAAGCTGAGGACCAACTGAAGGCCATTCCCCAGTTCTGTTTTCCCGATGCCAAGGAT
  TGGGTTCCTGTCCAGCAGTTCACCAGTGAAACATTCTCGTTTGTCTTAACTGGAGAAGATGGGAGCAGA
  AGGTTCGGTTACTGCCGAAGACTGCTGCCTGGAGGCAAAGGGAAGCGCCTTCCTGAAGTTTACTGCATT
  GTGAGCCGCCTGGGATGCTTCAGCCTCTTTTCAAGGATCTTGGATGAGGTGGAAAAAAGACGAGGCATC
  TCTCCTGCCCTGGTTCAGCCACTCATGAGAAGTGTCATGGAAGCCCCTTTCCCAGCCCTGGGCAAAACC
  ATCCTTGTCAAGAACTTCCTGCCAGGTTCAGGAACTGAGGTGATCGAACTGTGCCGCCCGCTGGACTCC
  CGGCTCGAGCACGTGGACTTTGAGTCTCTCTTCTCCTCCCTCAGCGTCCGCCACCTGGTCTGTGTGTTT
  GCCTCCCTGCTTCTGGAGAGGAGGGTCATCTTCATTGCAGACAAGCTCAGCATCCTGTCCAAGTGCTGC
  CACGCGATGGTGGCGCTGATCTACCCCTTCGCCTGGCAGCACACCTACATCCCGGTGCTGCCACCCGCC
  ATGGTCGACATCGTGTGCTCGCCGACGCCCTTCCTCATCGGGCTGCTCTCCAGCTCGCTGCCACTGCTC
  AGGGAGCTGCCGCTGGAAGAGGTCCTTGTGGTTGACCTCGTCAACAGCCGGTTCCTCAGACAGATGGAC
  GATGAGGACTCCATCCTGCCCCGGAAGCTTCAGGTGGCCCTGGAACACATTCTGGAACAGAGGAACGAG
  CTGGCTTGTGAGCAGGACGAAGGGCCCCTAGACGGCAGGCACGGTCCAGAGTCCAGCCCCTTGAACGAG
  GTGGTGTCTGAAGCCTTTGTCCGCTTCTTCGTGGAGATTGTGGGACACTACTCTTTGTTCCTGACGTCG
  GGCGAGCGTGAGGAGAGAACCCTGCAGCGGGAGGCCTTCCGCAAAGCTGTCTCCTCCAAGAGCCTCCGC
  CACTTCCTGGAGGTCTTCATGGAGACTCAGATGTTTCGGGGCTTCATCCAGGAGCGGGAGCTGCGCCGG
  CAGGATGCCAAAGGTCTGTTTGAGGTCCGAGCCCAAGAGTATCTGGAAACACTCCCCAGTGGAGAGCAC
  AGCGGTGTCAATAAGTTCCTGAAGGGACTAGGCAATAAAATGAAATTTCTCCACAAGAAATAA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_015689
• Insert Size: 3030 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015689.2
• RefSeq Size: 3485 bp
• RefSeq ORF: 3030 bp
• Locus ID: 27147
• UniProt ID: Q9ULE3
• Cytogenetics: 7q34
• MW: 113.9 kDa
• Gene Summary: Guanine nucleotide exchange factor (GEF) which may activate RAB9A and RAB9B. Promotes the exchange of GDP to GTP, converting inactive GDP-bound Rab proteins into their active GTP-bound form. May play a role in late endosomes back to trans-Golgi network/TGN transport.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (a). Variants 1, 2 and 5 encode the same isoform (a).