SPG48 (AP5Z1) (NM_014855) Human Untagged Clone

CAT#: SC304176

AP5Z1 (untagged)-Human KIAA0415 (KIAA0415)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SPG48
• Synonyms: KIAA0415; SPG48; zeta
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC304176 representing NM_014855.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGTTCTCGGCAGGAGCGGAGAGTTTGCTCCACCAGGCCAGGGAGATCCAGGACGAGGAGCTGAAGAAG
  TTCTGTTCCCGGATCTGTAAACTGCTGCAGGCGGAGGACTTGGGGCCGGACACCCTCGACTCCCTGCAG
  AGGCTCTTCCTCATCATCTCAGCCACGAAGTACAGCCGGAGGCTGGAGAAGACATGCGTAGACCTGCTG
  CAGGCCACCCTCGGCCTGCCTGCATGCCCCGAGCAGCTCCAGGTGCTTTGCGCCGCCATCCTGCGAGAG
  ATGTCCCCCTCTGACAGCCTCAGCCTGGCCTGGGACCACACGCAGAACAGCCGGCAGCTGAGCCTGGTG
  GCCTCCGTTCTCTTGGCCCAGGGTGACAGAAACGAGGAGGTCAGAGCCGTGGGCCAGGGCGTGCTACGA
  GCGCTGGAGAGCCGGCAGCCTGAGGGACCCAGCCTCAGACACCTCCTCCCCGTCATGGCCAAGGTCGTG
  GTCCTCAGCCCGGGCACCCTCCAGGAGGACCAGGCCACCCTGCTCAGCAAGCGGCTGGTCGACTGGCTG
  CGCTACGCCAGCCTCCAGCAAGGGCTCCCACACTCCGGCGGCTTCTTCTCCACGCCCAGGGCCCGGCAG
  CCGGGCCCCGTCACCGAGGTGGACGGGGCGGTAGCCACAGACTTCTTCACGGTGCTCTCCAGCGGCCAC
  CGCTTCACAGACGACCAGTGGCTGAACGTGCAGGCCTTCTCTATGCTGCGGGCGTGGCTGCTGCACAGC
  GGCCCCGAGGGCCCGGGCACCCTGGACACAGATGACAGGTCAGAGCAGGAGGGCTCCACTCTGTCGGTG
  ATCTCCGCCACCTCCTCTGCCGGCCGCCTGCTGCCGCCCCGGGAGCGGCTTCGGGAGGTGGCCTTCGAG
  TACTGCCAGCGCCTCATTGAGCAAAGTAACCGACGAGCCCTGAGGAAGGGGGACTCCGACCTGCAGAAA
  GCTTGCCTGGTGGAGGCCGTGCTGGTGCTGGACGTGCTGTGCCGGCAGGACCCGTCCTTCCTGTACCGA
  AGTCTCTCCTGCCTGAAGGCCCTGCACGGGCGGGTGCGCGGGGACCCGGCCTCTGTGCGGGTGCTGCTG
  CCCCTCGCCCACTTCTTCCTGAGCCACGGGGAAGCGGCTGCAGTGGACTCGGAAGCCGTCTACCAGCAC
  CTGTTCACCAGGATCCCGGTGGAGCAGTTCCACAGCCCCATGCTGGCCTTTGAATTCATCCAGTTCTGC
  AGGGACAACCTCCACCTGTTCAGCGGGCACCTCAGCACCCTCAGATTGAGCTTCCCCAACCTCTTTAAG
  TTCCTGGCCTGGAACAGCCCACCCCTCACCTCCGAGTTTGTGGCGCTCCTCCCGGCCCTGGTGGACGCT
  GGCACAGCCCTGGAGATGCTGCACGCGCTGCTGGACCTGCCCTGCTTGACGGCGGTGCTGGACCTGCAG
  CTCAGGTCAGCACCGGCTGCATCCGAGAGGCCACTCTGGGACACCTCTCTCAGGGCCCCCAGCTGCCTG
  GAGGCCTTCCGGGACCCGCAGTTCCAGGGTCTTTTCCAATACCTGCTGCGCCCCAAGGCCAGTGGCGCC
  ACTGAGAGGTTGGCGCCACTCCACCAGCTGCTGCAGCCCATGGCCGGCTGTGCCCGCGTGGCCCAGTGT
  GCCCAGGCCGTGCCCACGCTGCTGCAGGCATTCTTCTCAGCAGTGACCCAGGTGGCTGACGGGTCCCTG
  ATCAACCAGCTGGCGCTGCTGCTCCTGGGCAGGAGCGACTCGCTCTACCCGGCCCCAGGGTACGCTGCC
  GGTGTGCACAGTGTGCTGAGTTCTCAGTTCCTGGCCCTGTGTACGCTGAAACCCTCCCTGGTGGTGGAG
  CTGGCAAGAGACCTGCTGGAGTTCCTGGGCAGCGTGAATGGTCTCTGCAGCAGGGCGAGCCTCGTCACC
  AGCGTGGTGTGGGCCATCGGCGAGTACCTGTCGGTGACCTACGATCGGAGGTGCACCGTGGAGCAGATC
  AACAAGTTCTTCGAAGCCCTGGAGGCTCTGCTATTCGAGGTCACCCAGTGCCGCCCCTCTGCTGCCCTG
  CCCAGGTGTCCCCCCCAGGTGGTCACCGTGCTGATGACCACGCTGACGAAGCTGGCCTCCCGGAGCCAA
  GATCTGATCCCCAGGGCCTCTTTATTGCTGTCAAAGATGAGGACCCTGGCTCACAGTCCAGCCACCAGC
  TCCACGCACAGCGAGGAGGGCGCGGAAGCCATCCGTACCCGGGCCACAGAGCTGCTGACCCTGCTGAAG
  ATGCCTAGCGTGGCCCAGTTTGTGCTCACACCCAGCACGGAGGTGTGCAGCCCCCGCTATCACCGCGAT
  GCCAACACGGCCCTGCCCCTGGCCCTGCGCACGGTCAGCCGGCTGGTGGAGAGGGAGGCCGGCCTCATG
  CCAGGGTGA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_014855
• Insert Size: 2424 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014855.2
• RefSeq Size: 5519 bp
• RefSeq ORF: 2424 bp
• Locus ID: 9907
• UniProt ID: O43299
• Cytogenetics: 7p22.1
• MW: 88.6 kDa
• Gene Summary: This gene was identified by genome-wide screen for genes involved in homologous recombination DNA double-strand break repair (HR-DSBR). The encoded protein was found in a complex with other proteins that have a role in HR-DSBR. Knockdown of this gene reduced homologous recombination, and mutations in this gene were found in patients with spastic paraplegia. It was concluded that this gene likely encodes a helicase (PMID:20613862). [provided by RefSeq, Jan 2011]