STAG3 (NM_012447) Human Untagged Clone

CAT#: SC303984

STAG3 (untagged)-Human stromal antigen 3 (STAG3)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: STAG3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC303984 representing NM_012447.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGTCTTCCCCGTTGCAAAGAGCTGTGGGAGATACCAAGAGGGCCTTGTCTGCATCTTCTAGTTCCTCT
  GCCAGTCTACCCTTTGATGACAGGGACTCAAACCATACCTCAGAGGGGAATGGCGACTCTTTGTTAGCT
  GATGAAGACACTGACTTTGAAGACAGCTTGAATCGCAATGTGAAGAAGAGAGCAGCAAAACGACCACCG
  AAAACAACACCGGTGGCAAAACATCCAAAGAAAGGGTCCCGAGTGGTACATCGTCATAGCCGGAAACAG
  TCAGAGCCACCAGCCAATGATCTTTTCAATGCTGTGAAAGCCGCCAAAAGTGACATGCAGTCTTTGGTA
  GATGAGTGGCTGGATAGCTACAAGCAAGACCAGGATGCAGGATTTCTGGAGCTTGTTAACTTTTTCATC
  CAATCTTGCGGATGTAAAGGCATTGTGACCCCTGAGATGTTCAAGAAGATGTCCAACTCAGAGATCATC
  CAGCACCTAACAGAGCAGTTTAATGAGGACTCGGGGGACTACCCTCTCATAGCTCCAGGTCCATCCTGG
  AAGAAGTTCCAGGGCAGCTTCTGTGAATTTGTGAGGACATTGGTCTGTCAGTGCCAGTACAGCCTCCTC
  TATGATGGCTTCCCTATGGACGACCTCATCTCCCTGCTCACTGGCCTCTCAGACTCACAAGTCCGCGCC
  TTCCGTCACACTAGCACCCTGGCTGCTATGAAACTGATGACCTCCCTGGTAAAAGTTGCCCTCCAACTG
  AGTGTGCACCAAGATAACAATCAGCGTCAGTATGAGGCTGAAAGAAACAAGGGGCCAGGGCAGAGGGCA
  CCTGAGCGGCTGGAGAGCCTGTTGGAGAAACGCAAAGAGCTCCAAGAGCATCAAGAGGAGATTGAGGGG
  ATGATGAATGCCCTCTTCAGGGGTGTCTTTGTTCATCGGTACAGGGATGTCCTTCCTGAGATCCGTGCT
  ATCTGCATTGAGGAAATTGGGTGTTGGATGCAAAGCTACAGCACGTCTTTCCTCACCGACAGCTATTTA
  AAATATATTGGTTGGACTCTGCATGATAAGCACCGAGAAGTCCGCCTGAAGTGTGTGAAGGCCCTGAAA
  GGGCTGTACGGTAACCGGGACCTGACCACACGCCTGGAGCTCTTCACCAGCCGCTTCAAGGACCGGATG
  GTTTCCATGGTCATGGACAGAGAGTATGATGTGGCAGTGGAGGCTGTCAGATTACTGATACTTATCCTT
  AAGAACATGGAAGGGGTGCTGACGGACGCGGATTGTGAGAGCGTCTACCCAGTTGTGTATGCCTCTCAT
  CGAGGCCTGGCCTCTGCCGCAGGCGAATTTCTGTACTGGAAACTCTTCTACCCTGAGTGCGAGATAAGA
  ATGATGGGTGGAAGAGAGCAACGCCAGAGCCCAGGCGCCCAGAGGACTTTCTTCCAGCTTCTGCTGTCC
  TTCTTTGTGGAGAGCGAGCTCCATGACCACGCTGCTTACTTAGTAGACAGTCTGTGGGACTGTGCAGGG
  GCTCGGCTGAAGGACTGGGAGGGTCTGACAAGCCTGCTGCTGGAGAAGGACCAGAACCTGGGTGATGTG
  CAGGAGAGCACACTGATAGAAATCCTTGTGTCCAGTGCCCGGCAAGCTTCAGAGGGGCACCCGCCTGTG
  GGCCGGGTCACTGGGAGGAAGGGCTTAACCTCTAAGGAGCGCAAGACCCAAGCCGATGACAGGGTGAAG
  TTGACTGAGCACCTCATCCCCCTGCTGCCCCAGCTCCTGGCCAAGTTCTCAGCTGATGCAGAGAAGGTC
  ACTCCCCTGCTCCAGCTTCTCAGCTGCTTTGACCTCCACATCTACTGCACTGGGCGCTTGGAGAAGCAC
  CTGGAGCTGTTCCTGCAGCAACTCCAGGAGGTGGTGGTGAAGCATGCAGAGCCAGCGGTGCTTGAGGCT
  GGGGCGCATGCCCTCTACCTGCTCTGTAATCCCGAATTCACTTTCTTCAGCCGGGCGGACTTTGCCCGC
  AGCCAGCTAGTAGATTTGCTGACTGACCGCTTCCAGCAGGAGCTTGAAGAGCTGTTACAGTCGTCCTTC
  CTAGATGAGGATGAGGTATATAATCTGGCAGCCACTCTGAAACGCCTCTCTGCCTTCTACAACACTCAT
  GACCTGACTCGCTGGGAGCTCTATGAGCCATGTTGCCAACTCCTGCAGAAGGCTGTGGACACAGGAGAG
  GTTCCTCACCAGGTTATCCTGCCAGCCTTGACTCTTGTCTATTTTTCCATTCTCTGGACACTAACCCAC
  ATTTCTAAATCAGATGCTTCCCAGAAGCAGCTGTCGAGTTTGAGGGACAGAATGGTGGCCTTCTGTGAA
  CTCTGCCAGAGTTGCCTCTCAGATGTGGATACTGAGATCCAGGAGCAGGCTTTTGTCTTATTAAGTGAT
  CTACTTCTCATCTTTAGCCCTCAGATGATTGTTGGGGGCCGTGATTTCCTTAGGCCACTTGTCTTTTTT
  CCTGAAGCTACTCTCCAGTCTGAGCTAGCCAGCTTCCTCATGGACCACGTCTTCATCCAGCCGGGAGAC
  CTGGGCAGTGGTGATTCCCAGGAGGATCATTTACAGATAGAGCGGCTACACCAGCGGCGCCGCCTCCTA
  GCCGGGTTCTGCAAGCTGTTGCTTTATGGGGTGCTGGAGATGGATGCAGCCTCAGATGTTTTCAAACAC
  TACAACAAGTTCTACAATGACTATGGTGACATTATCAAGGAAACATTAACTAGAGCAAGGCAGATTGAC
  CGAAGTCATTGTTCCCGAATCCTGCTGCTGAGCCTCAAGCAGCTGTACACAGAACTGCTGCAGGAGCAT
  GGGCCCCAGGGCCTGAATGAGCTTCCTGCCTTCATCGAGATGAGGGACCTGGCCCGGAGGTTTGCCTTG
  AGTTTTGGACCCCAGCAGCTGCAGAACCGTGACCTCGTGGTCATGCTACACAAGGAAGGCATCCAGTTC
  TCCTTGTCTGAGCTTCCTCCAGCTGGCTCCTCCAATCAGCCTCCAAATCTGGCATTCCTGGAGCTCCTT
  TCAGAGTTTTCCCCCCGACTCTTCCATCAGGACAAGCAGCTTTTACTGTCCTATCTAGAAAAGTGCCTG
  CAGCATGTCTCCCAGGCACCTGGCCATCCCTGGGGCCCAGTCACCACCTACTGCCACTCCCTCAGCCCT
  GTGGAGAACACAGCAGAGACCAGCCCTCAGGTCCTCCCCAGCTCCAAGAGGAGGCGCGTTGAAGGGCCT
  GCCAAGCCTAACAGAGAGGACGTCTCCTCGTCCCAGGAAGAAAGTCTGCAGCTGAACAGCATCCCGCCC
  ACGCCCACCCTCACCTCCACAGCTGTGAAGAGCAGGCAGCCCCTGTGGGGGTTGAAAGAGATGGAGGAA
  GAAGATGGCTCAGAGTTGGATTTTGCCCAGGGTCAGCCCGTCGCAGGCACCGAGAGGTCAAGGTTCTTG
  GGTCCACAATATTTCCAGACTCCACACAACCCTTCAGGTCCTGGCCTGGGCAACCAGCTGATGCGACTC
  AGCCTTATGGAAGAGGACGAGGAAGAAGAGTTAGAAATCCAGGATGAGTCAAATGAAGAACGGCAGGAT
  ACAGACATGCAAGCAAGTAGCTACTCTTCCACCAGTGAGCGCGGGCTGGACCTCTTAGATTCTACAGAG
  CTGGATATTGAGGATTTCTGA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_012447
• Insert Size: 3678 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.

Reference Data

• RefSeq: NM_012447.3
• RefSeq Size: 4394 bp
• RefSeq ORF: 3678 bp
• Locus ID: 10734
• UniProt ID: Q9UJ98
• Cytogenetics: 7q22.1
• Protein Pathways: Oocyte meiosis
• MW: 139 kDa
• Gene Summary: The protein encoded by this gene is expressed in the nucleus and is a subunit of the cohesin complex which regulates the cohesion of sister chromatids during cell division. A mutation in this gene is associated with premature ovarian failure. Alternate splicing results in multiple transcript variants encoding distinct isoforms. This gene has multiple pseudogenes. [provided by RefSeq, Apr 2014]
  Transcript Variant: This variant (1) represents the predominant transcript and encodes isoform 1. Variants 1 and 2 encode the same isoform.