SHANK2 (NM_012309) Human Untagged Clone

CAT#: SC303955

SHANK2 (untagged)-Human SH3 and multiple ankyrin repeat domains 2 (SHANK2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SHANK2
• Synonyms: AUTS17; CORTBP1; CTTNBP1; ProSAP1; SHANK; SPANK-3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC303955 representing NM_012309.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGCCGCGCAGCCCAACATCCAGCGAGGACGAGATGGCCCAGAGCTTCTCCGACTACTCCGTGGGGTCG
  GAGTCAGACAGCTCCAAAGAAGAGACCATCTATGACACGATCCGGGCCACTGCGGAGAAGCCGGGCGGT
  GCCAGGACGGAGGAGAGCCAGGGCAACACGCTGGTGATCCGCGTGGTCATCCATGACCTGCAGCAGACG
  AAATGCATTCGATTTAACCCGGATGCCACAGTGTGGGTTGCAAAGCAGCGGATCCTGTGTACATTAACC
  CAGAGTTTGAAAGATGTCCTGAACTACGGCCTGTTCCAGCCGGCCAGCAATGGGCGTGACGGCAAGTTC
  CTGGATGAGGAGCGGCTCCTGCGCGAGTACCCACAGCCCGTGGGTGAGGGCGTTCCTTCCCTGGAGTTT
  CGATACAAGAAGCGGGTGTATAAACAAGCCAGTCTCGATGAGAAACAGTTGGCCAAGCTCCACACGAAG
  ACCAATCTGAAGAAATGCATGGATCACATTCAGCATCGCTTGGTGGAGAAGATCACCAAGATGCTGGAC
  CGAGGCCTGGATCCCAATTTCCACGACCCGGAGACCGGAGAGACCCCCCTGACCTTAGCCGCTCAGCTG
  GACGACTCTGTGGAGGTCATCAAAGCTCTCAAAAATGGTGGAGCTCACCTGGACTTCCGTGCCAAAGAT
  GGGATGACCGCCCTACACAAAGCTGCCCGAGCGAGGAACCAAGTTGCCCTGAAGACCCTTTTAGAGCTT
  GGTGCATCCCCAGATTATAAAGACAGTTACGGCCTCACCCCGCTGTATCACACAGCCATCGTCGGAGGT
  GATCCCTACTGCTGCGAGCTTCTCCTGCACGAACACGCCACTGTGTGCTGCAAAGATGAGAACGGCTGG
  CACGAGATCCACCAGGCCTGCAGGTACGGGCACGTGCAGCACCTGGAGCACCTGCTGTTCTACGGGGCA
  GACATGAGTGCCCAGAATGCCTCGGGGAACACGGCCTTGCACATCTGCGCCCTCTACAACCAGGACAGC
  TGTGCAAGAGTGCTTCTGTTTCGAGGCGGAAATAAGGAGTTAAAAAACTACAACAGCCAGACTCCATTT
  CAGGTGGCCATAATAGCAGGCAACTTTGAGCTGGCAGAATACATCAAGAACCACAAGGAAACAGACATT
  GTGCCCTTCCGAGAGGCCCCGGCGTACTCCAACCGCCGGCGGCGGCCCCCCAACACGCTGGCCGCCCCC
  CGGGTCCTGCTGCGCTCCAACAGTGACAACAACCTCAATGCCAGCGCTCCCGACTGGGCCGTCTGCTCC
  ACGGCCACCTCGCACCGCAGCCTGTCACCCCAGCTGCTGCAGCAGATGCCCAGCAAGCCCGAGGGGGCC
  GCGAAGACCATTGGGAGCTACGTGCCCGGGCCCCGCAGCCGGTCCCCATCGCTCAACAGGCTGGGCGGC
  GCAGGCGAGGACGGCAAGAGGCCGCAGCCTCTCTGGCATGTCGGGTCGCCTTTTGCTCTTGGTGCCAAC
  AAGGACTCACTCTCGGCCTTCGAGTACCCGGGGCCCAAGCGGAAGCTCTACAGTGCCGTGCCCGGGAGG
  CTCTTCGTCGCTGTCAAGCCATACCAACCCCAAGTGGACGGCGAGATCCCCCTTCACCGCGGTGACAGG
  GTCAAAGTTCTGAGCATCGGTGAAGGGGGCTTCTGGGAAGGCAGCGCCCGCGGCCACATCGGATGGTTT
  CCGGCGGAGTGCGTGGAGGAGGTCCAGTGTAAGCCCAGGGACAGCCAGGCAGAAACCCGCGCTGACCGC
  AGCAAGAAGCTTTTCAGGCACTACACCGTGGGCTCCTATGACAGCTTCGACACTTCCAGTGACTGCATT
  ATTGAGGAGAAGACGGTGGTCCTGCAGAAAAAAGACAATGAGGGCTTTGGATTCGTGCTTCGAGGGGCC
  AAAGCTGACACACCCATTGAAGAATTCACACCAACACCGGCTTTCCCAGCCCTACAGTACCTGGAGTCC
  GTGGATGAAGGTGGGGTGGCGTGGCAAGCCGGACTAAGGACCGGGGACTTCTTGATTGAGGTTAACAAT
  GAGAATGTTGTCAAAGTCGGCCACAGGCAGGTGGTGAACATGATCCGGCAGGGAGGGAATCACCTGGTC
  CTTAAGGTGGTCACGGTGACCAGGAATCTGGACCCCGACGACACCGCCAGGAAGAAAGCTCCCCCGCCT
  CCAAAGCGGGCACCGACCACAGCCCTCACCCTGCGCTCCAAGTCCATGACCTCGGAGCTGGAGGAGCTC
  GTGGATAAAGCCTCGGTCCGGAAGAAGAAGGATAAACCCGAGGAGATAGTCCCGGCCTCCAAGCCCTCC
  CGCGCTGCTGAGAACATGGCTGTGGAACCGAGGGTGGCGACCATCAAGCAGCGGCCCAGCAGCCGGTGC
  TTCCCGGCGGGCTCAGACATGAACTCTGTGTACGAACGCCAAGGAATCGCCGTGATGACGCCCACTGTT
  CCTGGGAGCCCAAAAGCCCCGTTTCTGGGCATCCCTCGAGGTACGATGCGAAGGCAGAAATCAATAGAC
  AGCAGAATCTTTCTATCAGGAATAACAGAGGAAGAGCGGCAGTTTCTGGCTCCTCCAATGCTGAAGTTC
  ACCAGAAGCCTGTCCATGCCGGACACCTCTGAGGACATCCCCCCTCCACCGCAGTCTGTGCCCCCGTCC
  CCACCACCACCTTCCCCAACCACTTACAACTGCCCCAAGTCCCCAACTCCAAGAGTCTACGGGACGATT
  AAGCCTGCGTTCAATCAGAATTCTGCCGCCAAGGTGTCCCCCGCCACCAGGTCCGACACCGTGGCCACC
  ATGATGAGGGAGAAGGGGATGTACTTCAGGAGAGAGCTGGACCGCTACTCCTTGGACTCTGAAGACCTC
  TACAGTCGGAATGCCGGCCCGCAAGCCAACTTCCGCAACAAGAGAGGCCAGATGCCAGAAAACCCATAC
  TCAGAGGTGGGGAAGATCGCCAGCAAAGCCGTCTACGTCCCCGCCAAGCCCGCCAGGCGGAAGGGGATG
  CTGGTGAAGCAGTCCAACGTGGAGGACAGCCCCGAGAAGACGTGCTCCATCCCTATCCCGACCATCATC
  GTGAAGGAGCCGTCCACCAGCAGCAGCGGCAAGAGCAGCCAGGGCAGCAGCATGGAGATCGACCCCCAG
  GCCCCGGAGCCACCGAGCCAGCTGCGGCCTGACGAAAGCCTGACCGTCAGCAGCCCCTTTGCCGCCGCC
  ATCGCCGGAGCCGTCCGCGACCGTGAGAAGCGGCTGGAAGCCAGGAGGAACTCCCCGGCCTTCCTCTCC
  ACAGACCTGGGGGATGAGGATGTGGGCCTGGGGCCACCCGCCCCCAGGACGCGGCCCTCCATGTTCCCC
  GAGGAGGGGGATTTTGCTGACGAGGACAGCGCTGAGCAGCTGTCATCCCCCATGCCGAGTGCCACGCCC
  AGGGAGCCCGAAAACCATTTCGTGGGTGGCGCCGAGGCCAGTGCTCCGGGTGAGGCTGGGAGGCCGCTG
  AATTCCACGTCCAAAGCCCAGGGGCCCGAGAGCAGCCCAGCAGTGCCCTCCGCGAGCAGCGGCACAGCC
  GGCCCCGGGAATTATGTCCACCCACTCACAGGGCGGCTGCTTGATCCCAGCTCCCCGCTGGCCCTGGCA
  CTCTCCGCAAGGGACCGAGCCATGAAGGAGTCTCAACAGGGACCCAAAGGGGAGGCCCCCAAGGCCGAC
  CTCAACAAACCTCTTTACATTGATACCAAAATGCGGCCCAGCCTGGATGCCGGCTTCCCTACGGTCACC
  AGGCAGAACACCCGGGGACCCCTGAGGCGGCAGGAGACGGAGAACAAGTACGAGACCGACCTGGGCCGA
  GACCGGAAAGGCGATGACAAGAAGAACATGCTGATCGACATCATGGACACGTCCCAGCAGAAGTCGGCT
  GGCCTGCTGATGGTGCACACCGTGGACGCCACTAAGCTGGACAACGCCCTGCAGGAAGAGGACGAGAAG
  GCAGAGGTGGAGATGAAGCCAGACAGCTCGCCGTCCGAGGTGCCAGAAGGTGTTTCCGAAACCGAAGGT
  GCTTTACAGATCTCCGCTGCCCCCGAGCCCACCACCGTGCCCGGCAGAACCATCGTCGCGGTGGGCTCC
  ATGGAAGAGGCGGTGATTTTGCCATTCCGCATCCCTCCTCCCCCTCTGGCATCCGTGGACTTGGATGAG
  GATTTTATTTTTACAGAGCCATTGCCTCCTCCCCTGGAATTTGCAAATAGTTTTGATATCCCCGATGAC
  CGGGCAGCTTCTGTCCCGGCTCTCTCAGACTTAGTGAAGCAGAAGAAAAGCGACACCCCTCAGTCCCCT
  TCGTTGAACTCCAGCCAACCAACCAACTCTGCAGACAGCAAGAAGCCAGCCAGTCTTTCAAACTGTCTG
  CCTGCCTCATTCCTGCCACCCCCTGAAAGCTTTGACGCCGTCGCCGACTCTGGGATCGAGGAGGTGGAC
  AGCCGGAGTAGCAGCGACCACCACCTCGAGACGACCAGCACTATCTCCACCGTGTCTAGCATCTCCACC
  CTGTCTTCCGAAGGTGGAGAGAATGTGGACACCTGCACAGTCTATGCAGATGGGCAAGCATTTATGGTT
  GACAAACCCCCAGTACCTCCTAAGCCAAAAATGAAGCCCATCATTCACAAAAGCAATGCACTTTATCAA
  GACGCGCTCGTGGAAGAAGATGTAGATAGCTTTGTTATCCCCCCGCCCGCTCCCCCGCCCCCGCCGGGC
  AGTGCCCAGCCTGGGATGGCCAAGGTTCTCCAGCCAAGGACCTCCAAGTTGTGGGGCGACGTCACAGAG
  ATCAAAAGCCCGATTCTCTCAGGCCCAAAGGCAAACGTTATTAGTGAATTGAACTCTATCCTACAGCAA
  ATGAACCGAGAGAAATTGGCAAAGCCGGGGGAAGGACTGGATTCACCAATGGGAGCCAAGTCCGCCAGC
  CTCGCTCCAAGAAGCCCGGAGATCATGAGCACCATCTCAGGTACACGGAGCACGACGGTCACCTTCACT
  GTTCGCCCCGGCACCTCCCAGCCCATCACCCTGCAGAGCCGGCCCCCCGACTATGAAAGCAGGACCTCA
  GGAACAAGACGTGCCCCAAGCCCTGTGGTCTCGCCAACAGAGATGAACAAAGAGACCCTGCCCGCCCCC
  CTGTCTGCTGCCACCGCCTCTCCTTCTCCCGCTCTCTCAGATGTCTTTAGCCTTCCAAGCCAGCCCCCT
  TCTGGGGATCTATTTGGCTTGAACCCAGCGGGACGCAGTAGGTCGCCATCCCCCTCGATACTGCAACAG
  CCAATCTCAAATAAGCCTTTTACAACTAAACCTGTCCACCTGTGGACTAAACCAGATGTGGCCGATTGG
  CTGGAAAGTCTAAACTTGGGTGAACATAAAGAGGCCTTCATGGACAATGAGATCGATGGCAGTCACTTA
  CCAAACCTGCAGAAGGAGGACCTCATCGATCTTGGGGTAACTCGAGTCGGGCACAGAATGAACATAGAA
  AGGGCTTTGAAACAGCTGCTGGACAGATAA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_012309
• Insert Size: 5550 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012309.4
• RefSeq Size: 10675 bp
• RefSeq ORF: 5550 bp
• Locus ID: 22941
• UniProt ID: Q9UPX8
• Cytogenetics: 11q13.3-q13.4
• MW: 201.3 kDa
• Gene Summary: This gene encodes a protein that is a member of the Shank family of synaptic proteins that may function as molecular scaffolds in the postsynaptic density of excitatory synapses. Shank proteins contain multiple domains for protein-protein interaction, including ankyrin repeats, and an SH3 domain. This particular family member contains a PDZ domain, a consensus sequence for cortactin SH3 domain-binding peptides and a sterile alpha motif. The alternative splicing demonstrated in Shank genes has been suggested as a mechanism for regulating the molecular structure of Shank and the spectrum of Shank-interacting proteins in the postsynaptic densities of the adult and developing brain. Alterations in the encoded protein may be associated with susceptibility to autism spectrum disorder. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1).